LMPD Database

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LMP010113

UniProt Annotations

Entry Information
Gene NameDELLA protein RGL2
Protein EntryRGL2_ARATH
UniProt IDQ8GXW1
SpeciesArabidopsis
Comments
Comment typeDescription
Disruption PhenotypeHigher germination rate in the presence of glucose and at supraoptimal temperature conditions. Rga, gai, rgl1, rgl2 and rgl3 pentuple mutant displays constitutive GA responses even in the absence of GA treatment. {ECO:0000269|PubMed:16916886, ECO:0000269|PubMed:18162586, ECO:0000269|PubMed:23482857}.
DomainThe DELLA motif is required for its GA-induced degradation but not for the interaction with GID2.
FunctionProbable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. No effect of the BOI proteins on its stability. Probably acts by participating in large multiprotein complexes that repress transcription of GA- inducible genes. Upon GA application, it is degraded by the proteasome, allowing the GA signaling pathway. Acts as a major GA- response repressor of seed germination, including seed thermoinhibition. Promotes the biosynthesis of abscisic acid (ABA), especially in seed coats to maintain seed dormancy. Delays flowering and adult leaf production. Also regulates the floral development, petal, stamen and anther development, by repressing the continued growth of floral organs. Its activity is probably regulated by other phytohormones such as auxin and ethylene. Involved in the regulation of seed dormancy and germination, including glucose-induced delay of seed germination. Promotes salt tolerance. Acts as a repressor of positive regulators of trichome initiation. Required during the flagellin-derived peptide flg22- mediated growth inhibition. Contributes to the susceptibility to the biotrophic pathogen P.syringae pv. tomato and to the resistance to the necrotrophic pathogens B.cinerea A.brassicicola, probably by repressing the SA-defense pathway and preventing cell death. Prevents stress-induced reactive oxygen species (ROS) accumulation (e.g. salt stress) by acting on the ROS scavenging system, and delays ROS-induced cell death, thus promoting stress tolerance. {ECO:0000269|PubMed:11877383, ECO:0000269|PubMed:12610625, ECO:0000269|PubMed:14615596, ECO:0000269|PubMed:14973286, ECO:0000269|PubMed:15128937, ECO:0000269|PubMed:16034591, ECO:0000269|PubMed:16400150, ECO:0000269|PubMed:16916886, ECO:0000269|PubMed:17141619, ECO:0000269|PubMed:17384169, ECO:0000269|PubMed:17704233, ECO:0000269|PubMed:18162586, ECO:0000269|PubMed:18450450, ECO:0000269|PubMed:18450451, ECO:0000269|PubMed:18941053, ECO:0000269|PubMed:20093430, ECO:0000269|PubMed:20956298}.
InductionUp-regulated transiently following seed imbibition to decline rapidly as germination proceeds; this induction is delayed at supraoptimal temperature conditions (e.g. 34 degrees Celsius). Accumulates in seed coats of dormant seeds where germination does not occur after imbibition. Increased levels upon abscisic acid (ABA) treatment. Down-regulated by norflurazon (NF), an ABA biosynthesis inhibitor. Induced by stress such as glucose, salt or mannitol treatment. {ECO:0000269|PubMed:11877383, ECO:0000269|PubMed:16916886, ECO:0000269|PubMed:17384169, ECO:0000269|PubMed:18162586, ECO:0000269|PubMed:18450450, ECO:0000269|PubMed:18941053, ECO:0000269|PubMed:20956298}.
InteractionQ9MAA7:GID1A; NbExp=3; IntAct=EBI-963665, EBI-963597; Q940G6:GID1C; NbExp=3; IntAct=EBI-963665, EBI-963794;
PtmPhosphorylated. Phosphorylation on Tyr residues is required for proteasome-mediated degradation in response to gibberellic acid (GA). Dephosphorylation may be prerequisite for its degradation by the proteasome. {ECO:0000269|PubMed:16167898, ECO:0000269|PubMed:17333251}.
PtmUbiquitinated (Probable). Upon GA application or seed imbibation, it is ubiquitinated by the SCF(GID2) complex, leading to its subsequent degradation. {ECO:0000305}.
SimilarityBelongs to the GRAS family. DELLA subfamily. {ECO:0000305}.
Subcellular LocationNucleus {ECO:0000269|PubMed:20093430}.
SubunitInteracts directly with the GID2/SLY1 component of the SCF(GID2) complex. Interacts (via N-terminus) with GID1A, GID1B and GID1C (via N-terminus). Binds to bHLH transcription factors such as PIF1, PIF4, PIF6 and SPT. Interacts with the BOI proteins BOI, BRG1, BRG2 and BRG3. {ECO:0000269|PubMed:15173565, ECO:0000269|PubMed:16709201, ECO:0000269|PubMed:19500306, ECO:0000269|PubMed:20093430, ECO:0000269|PubMed:23482857}.
Tissue SpecificityPredominantly expressed in germinating seeds, flowers and siliques. Only detectable in the inflorescence, with high levels in young flower buds and significant levels in siliques. In imbibed seeds, it is restricted to seed coats, elongating regions of pre-emergent and recently emerged radicles, endosperm (especially micropylar endosperm), and embryonic axis. Not expressed in leaves, bolting stems or roots. {ECO:0000269|PubMed:11877383, ECO:0000269|PubMed:18941053, ECO:0000269|PubMed:19500306, ECO:0000269|PubMed:20093430, ECO:0000269|PubMed:20956298}.
Web ResourceName=Protein Spotlight; Note=Kiss of life - Issue 137 of April 2012; URL="http://web.expasy.org/spotlight/back_issues/137";