LMPD Database

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LMP011436

UniProt Annotations

Entry Information
Gene Namevery-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase PASTICCINO 2
Protein EntryPAS2_ARATH
UniProt IDQ8VZB2
SpeciesArabidopsis
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q8VZB2-1; Sequence=Displayed; Name=2; IsoId=Q8VZB2-2; Sequence=VSP_037155, VSP_037156; Note=Derived from EST data. No experimental confirmation available.;
Catalytic ActivityA very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain trans-2,3-dehydroacyl-CoA + H(2)O.
CautionShares some similarity with tyrosine phosphatase proteins but it has probably no phosphatase activity since the potential active site Cys residue in position 65 is replaced by Gly. {ECO:0000305}.
Disruption PhenotypeEmbryo lethal when homozygote. {ECO:0000269|PubMed:18799749, ECO:0000269|PubMed:21896643}.
FunctionResponsible for the dehydration step in very long-chain fatty acid (VLCFA) synthesis. May be an anti-phosphatase that prevents CDKA-1 dephosphorylation and activation. Involved in the hormonal control of cell division and differentiation. Required for proliferation control of meristematic and non-meristematic cells. Negative regulator of the cell cycle. {ECO:0000269|PubMed:12459923, ECO:0000269|PubMed:12472687, ECO:0000269|PubMed:12857804, ECO:0000269|PubMed:16698944, ECO:0000269|PubMed:18799749, ECO:0000269|PubMed:21896643}.
MiscellaneousPlants with reduced exprssion of PAS2 show impaired embryo and seedling development associated with cell de- differentiation and proliferation. Root growth is reduced, due to a delay in cell plate establishment during cytokinesis (PubMed:21896643). {ECO:0000305|PubMed:21896643}.
PathwayLipid metabolism; fatty acid biosynthesis.
Sequence CautionSequence=CAB89395.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
SimilarityBelongs to the very long-chain fatty acids dehydratase HACD family. {ECO:0000305}.
Subcellular LocationEndoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Cytoplasm. Nucleus. Note=Found in the cytoplasm of dividing cells but moves into the nucleus upon cell differentiation.
SubunitInteracts with CDKA-1; but only with the 'Tyr-15' phosphorylated protein. Interacts with PAS1. Part of the fatty acid elongase complex which contains a beta-ketoacyl-CoA synthase (KCS), a beta-ketoacyl-CoA reductase (KCR), a beta-hydroxyacyl-CoA dehydratase (HCD) and an enoyl-CoA reductase (ECR) (Probable). {ECO:0000305|PubMed:16698944, ECO:0000305|PubMed:20145257}.
Tissue SpecificityHigh expression in young seedlings, roots, root tips, flowers and young siliques. Lower levels in leaves and stems. {ECO:0000269|PubMed:12472687, ECO:0000269|PubMed:21896643}.