LMPD Database

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LMP012754

UniProt Annotations

Entry Information
Gene Namecubilin (intrinsic factor-cobalamin receptor)
Protein EntryCUBN_RAT
UniProt IDO70244
SpeciesRat
Comments
Comment typeDescription
Developmental StageExpressed at 6 dpc in primitive endoderm cells, in apical membrane invaginations, in endocytic vesicles, endoplasmic reticulum and Golgi apparatus. At the egg cylinder stage (7-8 dpc), expressed in visceral and parietal endoderm. From the early headfold stage (8.9 dpc), expressed in ectodermal cells lining the proamniotic cavity. At 10 dpc, detected in the newly forming neuroepithelium
DomainThe CUB domains 5 to 8 mediate binding to GIF and ALB. CUB domains 1 and 2 mediate interaction with LRP2
FunctionCotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. {ECO:0000269|PubMed:10811843, ECO:0000269|PubMed:11805171, ECO:0000269|PubMed:12724130, ECO:0000269|PubMed:15616221, ECO:0000269|PubMed:9153271, ECO:0000269|PubMed:9478979}.
InteractionSelf; NbExp=2; IntAct=EBI-3954161, EBI-3954161;
PtmN-glycosylated
PtmThe precursor is cleaved by a trans-Golgi proteinase furin. The result is a propeptide cleaved off (By similarity)
SimilarityContains 27 CUB domains. {ECO:0000255|PROSITE- ProRule:PRU00059}.
SimilarityContains 7 EGF-like domains. {ECO:0000255|PROSITE- ProRule:PRU00076}.
Subcellular LocationEndosome membrane ; Peripheral membrane protein . Lysosome membrane ; Peripheral membrane protein . Note=Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells.
SubunitComponent of the cubam complex composed of CUBN and AMN (By similarity). The cubam complex can oligomerize and form cubam trimers. Interacts with LRP2 in a dual-receptor complex in a calcium-dependent manner. Found in a complex with PID1/PCLI1, LRP1 and CUBNI (By similarity). Interacts with LRP1 and PID1/PCLI1 (By similarity)
Tissue SpecificityExpressed to intestinal, renal and yalk sac apical membranes. In kidney, expressed in the proximal tubule