Lipidomics studies on macrophages - bone marrow-derived macrophages treated with Kdo2-Lipid A
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| Legend: |  : View data for technical replicates : View graphs of average of replicates |
|---|
| Details/Graph technical replicates | LM_ID | Name | 0 hrs | 6 hrs | 24 hrs | Units | |
|---|---|---|---|---|---|---|---|
|
| LMST01020004 | CE(14:0) | 6 | 6.9 | 7.9 | pmol/1E6 cells | |
|
| LMST01020021 | CE(14:1) | - | - | - | pmol/1E6 cells | |
|
| LMST01020027 | CE(15:0) | 2.3 | 2.9 | 4.2 | pmol/1E6 cells | |
|
| LMST01020022 | CE(15:1) | - | - | - | pmol/1E6 cells | |
|
| LMST01020005 | CE(16:0) | 14 | 17 | 23 | pmol/1E6 cells | |
|
| LMST01020006 | CE(16:1) | 35 | 53 | 47 | pmol/1E6 cells | |
|
| LMST01020024 | CE(16:2) | 12 | 14 | 14 | pmol/1E6 cells | |
|
| LMST01020026 | CE(17:0) | - | - | - | pmol/1E6 cells | |
|
| LMST01020023 | CE(17:1) | 18 | 20 | 20 | pmol/1E6 cells | |
|
| LMST01020007 | CE(18:0) | 7.2 | 14 | 12 | pmol/1E6 cells | |
|
| LMST01020003 | CE(18:1) | 81 | 140 | 120 | pmol/1E6 cells | |
|
| LMST01020008 | CE(18:2) | 31 | 41 | 34 | pmol/1E6 cells | |
|
| LMST01020009 | CE(18:3) | 7.7 | 7.6 | 7.6 | pmol/1E6 cells | |
|
| LMST01020010 | CE(20:0) | 13 | 13 | 14 | pmol/1E6 cells | |
|
| LMST01020011 | CE(20:1) | 4.6 | 5.5 | 5.5 | pmol/1E6 cells | |
|
| LMST01020012 | CE(20:2) | 12 | 13 | 12 | pmol/1E6 cells | |
|
| LMST01020013 | CE(20:3) | 20 | 21 | 22 | pmol/1E6 cells | |
|
| LMST01020014 | CE(20:4) | 58 | 69 | 67 | pmol/1E6 cells | |
|
| LMST01020016 | CE(22:0) | 11 | 11 | 11 | pmol/1E6 cells | |
|
| LMST01020025 | CE(22:1) | 30 | 36 | 43 | pmol/1E6 cells | |
|
| LMST01020017 | CE(22:2) | 43 | 43 | 47 | pmol/1E6 cells | |
|
| LMST01020018 | CE(22:4) | 31 | 31 | 31 | pmol/1E6 cells | |
|
| LMST01020019 | CE(22:6) | 35 | 37 | 36 | pmol/1E6 cells |
*:
In HPLC/MS/MS measurements by multiple reaction monitoring, the response ratio for each analyte was determined relative to a known amount of internal standard (400 pmol of 13C18 (18:1) cholesterol ester) that was added to each biological and calibration sample. The absolute amount of each analyte in a sample was then calculated by comparison to a standard curve for a given analyte. Specific six point calibration curves from 0.0 to 1.6 nmoles were constructed for each set of samples for the following cholesterol esters: 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 20:0, 20:1, 20:2, 20:3, 20:4, and 22:6. Analytes for which no direct reference standard was available were determined based on the curve for a standard closest in number of carbon atoms and double bonds. The value reported in the table was finally adjusted for the amount of DNA measured for each sample.