Lipidomics studies on macrophages - bone marrow-derived macrophages treated with Kdo₂-Lipid A

*: The units in the table and graphs correspond to the pmol of each species which was determined by dividing the areas under the LC MS/MS elution curve for each species by that for an appropriate deuterated surrogate standard then multiplied by the amount of the surrogate standard added (~10 pmol to 2 nmol depending on the compound of interest). For most of the analytes, the intensities were similiar for equivalent amounts of the analyte as for the internal standard when the LC MS/MS parameters were optimized and quantitative analysis was conducted by Multiple Reaction Monitoring (MRM). If necessary, small corrections were made using empirically determined relative response factors (RRF). The pmoles were then normalized to the measured quantity of DNA present in each sample (i.e. dish of RAW cells at time zero or at varying times following treatment with and without Kdo2 lipid A).