Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo₂-Lipid A

Introduction | Browse | Search | Download

---

Mouse-over the above links for more information

Introduction

Experiments were conducted with RAW264.7 cells fed 10% fetal calf serum, as per protocol PP0000001004 (pdf). Measurements were taken at 0, 0.5, 1, 2, 4, 8, 12, and 24hrs for both Kdo₂-Lipid A-treated and control cells. Three separate experiments (biological replicates) were carried out, and MS measurements were performed in triplicate (technical replicates).

References

A Mouse Macrophage Lipidome Dennis EA, Deems RA, Harkewicz R, Quehenberger O, Brown HA, Milne SB, Myers DS, Glass CK, Hardiman GT, Reichart D, Merrill AH, Sullards MC, Wang E, Murphy RC, Raetz CR, Garrett T, Guan Z, Ryan AC, Russell DW, McDonald JG, Thompson BM, Shaw WA, Sud M, Zhao Y, Gupta S, Maurya MR, Fahy E, Subramaniam S. J Biol Chem (2010) [Epub ahead of print]
PMID:20923771

Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4. Raetz C, Garrett T, Reynolds C, Shaw W, Moore J, Smith JD, Ribeiro A, Murphy R, Ulevitch R, Fearns C, Reichart D, Glass C, Benner C, Subramaniam S, Harkewicz R, Bowers-Gentry R, Buczynski M, Cooper J, Deems R and Dennis E. Journal of Lipid Research 47, 1097-1111 (2006)
PMID:16479018 [doi:10.1194/jlr.M600027-JLR200]