Lipidomics studies on macrophages - Foam cell study with wild-type and LDLR(-/-) mice, normal and high-fat diets
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| Legend: |  : View data for technical replicates : Graph of technical replicates(within an expt.)
 : Graph of biological replicates(across expts.) |
|---|
| Details/Graph technical replicates | LM_ID | Name | Mass (neutral) |
WT-Fat WT-Chow KO-Fat KO-Chow |
Units . . . . | |
|---|---|---|---|---|---|---|
|
(BCJ070123) | LMSP02010004 | C16 Cer | 537.5 (ions) | 470 400 580 390 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02010006 | C18 Cer | 565.5 (ions) | 14 15 28 15 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02010007 | C20 Cer | 593.6 (ions) | 7.7 5.2 9.5 6 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02010008 | C22 Cer | 621.6 (ions) | 48 36 63 42 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02010009 | C24:1 Cer | 647.6 (ions) | 640 590 730 560 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02010012 | C24 Cer | 649.6 (ions) | 130 110 200 120 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02010010 | C26:1 Cer | 675.7 (ions) | 9.1 6.5 29 7.6 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02010011 | C26 Cer | 677.7 (ions) | 2.9 2.4 6.4 3 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010003 | C16 Sphingomyelin | 702.6 (ions) | 15650 14610 16290 14700 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010001 | C18 Sphingomyelin | 730.6 (ions) | 240 180 210 190 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010005 | C20 Sphingomyelin | 758.6 (ions) | 65 55 67 58 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010006 | C22 Sphingomyelin | 786.7 (ions) | 440 390 470 440 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010007 | C24:1 Sphingomyelin | 812.7 (ions) | 4750 5110 4520 5350 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010008 | C24 Sphingomyelin | 814.7 (ions) | 960 1010 970 1050 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010009 | C26:1 Sphingomyelin | 840.7 (ions) | 21 26 20 26 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010010 | C26 Sphingomyelin | 842.7 (ions) | 4.7 6.1 5.2 6.2 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA03 | C16 GlcCer | 699.6 (ions) | 1060 840 890 860 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA05 | C18 GlcCer | 727.6 (ions) | 33 20 20 20 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA06 | C20 GlcCer | 755.6 (ions) | 15 11 11 18 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA07 | C22 GlcCer | 783.7 (ions) | 150 130 130 140 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA08 | C24:1 GlcCer | 809.7 (ions) | 860 780 650 770 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA09 | C24 GlcCer | 811.7 (ions) | 250 280 270 320 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA10 | C26:1 GlcCer | 837.7 (ions) | 6.6 6.1 5 5.5 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA11 | C26 GlcCer | 839.7 (ions) | 2.5 3.6 2.5 4 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02020001 | C16DH Cer | 539.5 (ions) | 480 260 530 240 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02020008 | C18DH Cer | 567.6 (ions) | 21 19 47 17 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02020009 | C20DH Cer | 595.6 (ions) | 11 5.5 14 9.4 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02020010 | C22DH Cer | 623.6 (ions) | 26 18 45 21 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02020011 | C24:1DH Cer | 649.6 (ions) | 160 71 160 89 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02020012 | C24DH Cer | 651.7 (ions) | 34 20 61 21 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02020013 | C26:1DH Cer | 677.7 (ions) | 4.9 3.5 7.9 5.1 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP02020014 | C26DH Cer | 679.7 (ions) | 1.9 0.87 4.5 1.2 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010004 | C16DH Sphingomyelin | 704.6 (ions) | 1920 1680 1780 1600 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010020 | C18DH Sphingomyelin | 732.6 (ions) | 38 30 32 28 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010021 | C20DH Sphingomyelin | 760.6 (ions) | 39 35 46 34 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010022 | C22DH Sphingomyelin | 788.7 (ions) | 37 39 39 40 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010023 | C24:1DH Sphingomyelin | 814.7 (ions) | 910 940 860 920 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010024 | C24DH Sphingomyelin | 816.7 (ions) | 43 44 45 47 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010025 | C26:1DH Sphingomyelin | 842.7 (ions) | 4.6 6 3.8 6.2 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP03010026 | C26DH Sphingomyelin | 844.7 (ions) | 1.7 2.4 2.4 2.9 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA04 | C16DH GlcCer | 701.6 (ions) | 78 120 100 94 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA19 | C18DH GlcCer | 729.6 (ions) | 0.95 2.3 0.4 3.8 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA20 | C20DH GlcCer | 757.6 (ions) | 9 3.2 5.5 1.8 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA21 | C22DH GlcCer | 785.7 (ions) | 12 15 7.9 15 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA22 | C24:1DH GlcCer | 811.7 (ions) | 36 62 25 23 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA23 | C24DH GlcCer | 813.7 (ions) | 13 25 13 8.9 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA24 | C26:1DH GlcCer | 839.7 (ions) | 3.3 1.5 - - |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP0501AA25 | C26DH GlcCer | 841.7 (ions) | 2.7 1.3 5.7 2.5 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP01020001 | Sphinganine | 301.3 (ions) | 39 13 42 11 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP01050002 | Sphinganine-P | 381.3 (ions) | 30 16 22 15 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP01010001 | Sphingosine | 299.3 (ions) | 450 220 420 230 |
pmol/1E6 cells | |
|
(BCJ070123) | LMSP01050001 | Sphingosine-P | 379.2 (ions) | 570 360 450 320 |
pmol/1E6 cells |
*:The units in the table and graphs correspond to the intensity of each species
divided by the intensity of an appropriate internal standard
(C12 analogs for Cer, GlcCer and SM; C20 analogs for So,Sa; C17 analogs for So-P and Sa-P).
The amount of internal standard added to each sample in the time series and control experiments was identical.
The relative intensity values were converted to pmoles and then normalized to the measured quantity of DNA present
in each RAW cell preparation following treatment with and without Kdo2 lipid A for each time point.