Lipidomics studies on macrophages - RAW 264.7 cells treated with Kdo2-Lipid A

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Fatty acids (FA) [cells]

Display Time Course Plots for Selected Analytes

Legendgraph_icon: View data/graphs for technical replicates
graph icon: Graph of biological replicates(across expts.)
Details/Graph
 Name Kdo
Ctl
 0min		 Kdo
Ctl
 30min		 Kdo
Ctl
 1hr		 Kdo
Ctl
 2hrs		 Kdo
Ctl
 4hrs		 Kdo
Ctl
 8hrs		 Kdo
Ctl
 12hrs		 Kdo
Ctl
 24hrs
Units
. . . .
 details icon   graph icon10Z-heptadecenoic acid-
0.05		 0.01
0.00		 0.00
0.11		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.01		 0.00
0.01		 pmol/ug DNA
 details icon   graph icon11,14,17-eicosatrienoic acid-
2.77		 17.27
3.31		 7.74
3.82		 4.86
2.26		 2.32
2.48		 1.87
1.83		 1.68
2.52		 1.25
3.51		 pmol/ug DNA
 details icon   graph icon11,14-eicosadienoic acid-
0.42		 0.38
0.30		 0.27
0.47		 0.24
0.20		 0.17
0.18		 0.04
0.10		 0.06
0.19		 0.09
0.16		 pmol/ug DNA
 details icon   graph icon13,16,19-docosatrienoic acid-
0.05		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 pmol/ug DNA
 details icon   graph icon13,16-docosadienoic acid-
0.04		 0.01
0.01		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 pmol/ug DNA
 details icon   graph icon5,8,11,14,17-eicosapentaenoic acid-
1.40		 3.02
1.36		 1.73
1.51		 1.09
1.04		 0.54
1.24		 0.26
0.64		 0.21
0.92		 0.22
0.78		 pmol/ug DNA
 details icon   graph icon5,8,11-eicosatrienoic acid-
0.72		 1.80
1.01		 1.45
1.02		 0.83
0.73		 0.35
0.56		 0.19
0.46		 0.15
0.62		 0.27
0.70		 pmol/ug DNA
 details icon   graph icon7,10,13,16,19-docosapentaenoic acid-
2.57		 4.19
3.27		 3.17
3.96		 2.36
2.23		 1.46
2.31		 0.62
1.47		 0.65
1.61		 0.82
1.50		 pmol/ug DNA
 details icon   graph icon7Z,10Z,13Z,16Z-docosatetraenoic acid-
1.11		 1.79
1.21		 1.45
1.76		 1.15
0.79		 0.62
0.85		 0.18
0.60		 0.19
0.74		 0.25
0.74		 pmol/ug DNA
 details icon   graph icon9Z-palmitoleic acid-
14.22		 21.20
16.70		 19.48
18.22		 14.81
13.15		 12.46
16.13		 17.01
11.13		 18.61
13.96		 16.22
9.62		 pmol/ug DNA
 details icon   graph iconArachidic acid-
0.84		 1.03
0.99		 1.12
0.83		 0.74
0.79		 1.02
1.01		 0.66
0.72		 0.90
0.39		 1.20
0.52		 pmol/ug DNA
 details icon   graph iconArachidonic acid-
13.02		 40.97
13.77		 20.99
19.14		 13.30
9.47		 5.74
9.52		 2.16
7.00		 2.01
7.99		 2.29
7.24		 pmol/ug DNA
 details icon   graph iconBehenic acid-
0.19		 0.26
0.21		 0.19
0.18		 0.16
0.12		 0.48
0.14		 0.13
0.11		 0.14
0.09		 0.21
0.11		 pmol/ug DNA
 details icon   graph iconCerotic acid-
0.06		 0.06
0.04		 0.03
0.01		 0.06
0.09		 1.57
0.12		 0.15
0.04		 0.42
0.12		 0.86
0.15		 pmol/ug DNA
 details icon   graph iconDHA-
4.48		 5.62
4.57		 4.21
6.93		 3.24
3.10		 2.28
3.13		 1.33
2.06		 1.35
2.10		 1.76
1.83		 pmol/ug DNA
 details icon   graph iconLauric acid-
11.62		 5.10
4.38		 5.77
8.41		 4.44
3.07		 6.29
1.12		 4.47
1.21		 1.50
1.94		 4.53
1.32		 pmol/ug DNA
 details icon   graph iconLignoceric acid-
0.38		 0.47
0.43		 0.38
0.38		 0.34
0.30		 0.83
0.37		 0.46
0.26		 0.71
0.39		 1.23
0.48		 pmol/ug DNA
 details icon   graph iconLinoleic acid-
7.63		 9.42
7.84		 12.18
7.83		 14.03
3.83		 26.75
4.63		 4.39
4.25		 2.03
3.01		 6.69
3.51		 pmol/ug DNA
 details icon   graph iconMargaric acid-
4.74		 5.38
5.13		 6.30
5.05		 3.59
4.51		 5.01
3.33		 2.36
2.07		 3.19
1.53		 3.13
2.01		 pmol/ug DNA
 details icon   graph iconMyristic acid-
23.83		 23.03
21.62		 23.23
23.63		 16.87
18.50		 18.53
14.62		 15.33
8.61		 18.09
10.50		 23.86
10.30		 pmol/ug DNA
 details icon   graph iconOleic acid-
85.50		 114.08
93.89		 94.14
109.48		 76.60
64.04		 82.96
70.85		 69.57
52.72		 74.39
63.56		 82.40
65.09		 pmol/ug DNA
 details icon   graph iconPalmitic acid-
172.38		 171.11
182.90		 160.89
211.50		 116.22
134.99		 132.68
107.85		 110.19
73.81		 140.65
80.36		 144.19
72.36		 pmol/ug DNA
 details icon   graph iconPentadecanoic acid-
5.35		 5.56
3.58		 5.74
5.41		 3.87
4.54		 5.71
3.23		 2.58
1.62		 2.57
1.86		 2.36
1.94		 pmol/ug DNA
 details icon   graph iconStearic acid-
96.14		 104.72
114.97		 113.27
108.42		 64.73
81.00		 67.74
62.01		 46.98
43.83		 71.12
31.65		 73.47
44.06		 pmol/ug DNA
 details icon   graph iconStearidonic acid-
0.05		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 pmol/ug DNA
 details icon   graph iconTricosanoic acid-
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 0.50
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.00		 pmol/ug DNA
 details icon   graph iconalpha-linolenic acid-
0.47		 0.50
0.35		 0.54
0.34		 0.54
0.15		 1.23
0.21		 0.12
0.15		 0.04
0.18		 0.14
0.14		 pmol/ug DNA
 details icon   graph iconbishomo-gamma-linolenic acid-
0.04		 0.00
0.00		 0.01
0.00		 0.00
0.00		 0.00
0.00		 0.00
0.01		 0.00
0.00		 0.00
0.02		 pmol/ug DNA
 details icon   graph iconcis-erucic acid-
0.20		 0.22
0.19		 0.16
0.17		 0.11
0.10		 0.08
0.11		 0.11
0.08		 0.17
0.19		 0.27
0.40		 pmol/ug DNA
 details icon   graph iconcis-selacholeic acid-
0.46		 0.61
0.62		 0.50
0.69		 0.39
0.32		 0.31
0.35		 0.39
0.27		 0.48
0.41		 0.66
0.58		 pmol/ug DNA
 details icon   graph icongamma-linolenic acid-
0.12		 0.17
0.31		 1.41
0.09		 1.76
0.08		 1.36
0.04		 0.07
0.10		 0.00
0.03		 0.61
0.06		 pmol/ug DNA
 details icon   graph iconDNA-
10.95		 11.37
11.27		 12.95
9.03		 14.27
12.98		 16.22
15.06		 17.47
22.15		 18.57
26.34		 16.99
40.76		 ug/sample
 details icon   graph iconTNF-
229.92		 391.26
242.70		 5322.56
276.59		 17063.56
324.70		 47210.22
391.59		 71522.67
429.26		 73184.89
569.81		 56696.00
587.92		 pg/ml


 

*:The units in the table and graphs correspond to the intensity of each analyte divided by the intensity of the corresponding deuterated internal standard. The amount of internal standard added to each sample in the time series and control experiments was identical. The relative intensity values were converted to pmoles by extrapolating from the standard curve for that analyte and then normalized to the number of cells in a given sample as measured by the quantity of DNA in the well.