Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

Averages of biological replicates - (GP) Glycerophospholipids Legend: : View data/graphs for replicates : View time course graphs for averages of biological replicates Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 PA | PC | PE | PG | PI | PS | DNA Details Time course graph (ave of biol. reps) LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - LPG(18:1) - 55.6 - 43.6 45.9 55.1 67.8 44.6 55.7 53 38.1 51 95.9 241 154 50 127 107 177 183 93.5 110 115 116 10.9 20.2 139 106 15.8 13.9 16.4 27.4 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(32:1) - 18.2 - 20.3 15.4 20.7 19.2 13.6 21.2 18.3 11.9 16.6 22.1 31 21.6 18 23 26.9 28.4 23.8 24 20 21.4 19.9 13.8 15 22.2 18.3 22.4 12.8 19.8 17.6 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(34:1) - 163 - 190 136 195 189 141 193 157 122 167 181 225 215 166 183 201 244 191 175 170 174 161 142 143 183 148 194 117 192 160 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(34:2) - 71.9 - 68.8 65.6 86.8 81.4 61.1 73.9 61.8 53.8 66 77 77.9 78 72.7 69.1 76.7 78.9 71.7 62.4 65.6 54.7 53.8 55.2 70.6 57.2 55.6 76.3 59.5 75 82.5 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(36:1) - 254 - 255 199 302 307 188 308 264 183 266 310 336 372 246 311 309 352 342 213 202 203 197 126 127 146 125 182 124 165 203 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(36:2) - 1002 - 903 850 1139 1158 844 1008 863 739 979 954 908 1061 1017 857 942 1012 907 679 795 606 710 685 821 636 599 865 712 833 1002 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(36:3) - 153 - 165 146 176 219 156 151 136 144 182 147 147 191 189 121 140 179 153 96.8 115 105 117 83.9 110 93.5 96.9 89.8 92.9 112 142 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(38:1) - 10.7 - 17 7.92 13.5 12.3 8.23 11 11 8.19 12.1 13.5 19.2 16.5 8.67 15.4 13.4 13.1 20 13.5 11.8 11.8 10.7 3.94 6.38 10.2 7.6 8.63 4.97 7.88 7.21 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(38:2) - 142 - 119 128 192 192 112 184 154 126 161 164 183 197 154 154 158 195 182 106 106 102 110 64 73.1 87 74.5 88 68.1 87.8 106 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(38:3) - 201 - 201 195 286 329 183 268 227 203 274 224 262 319 247 219 231 328 283 140 147 145 173 94 112 117 110 98 94.2 113 165 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(38:4) - 168 - 160 152 211 278 169 193 181 172 220 172 202 273 213 160 182 260 241 103 113 120 138 58.5 81.7 91 84.8 55.5 53.9 76 113 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(38:5) - 106 - 105 99.5 140 182 113 130 106 109 148 115 133 177 137 101 116 172 151 67.7 72.4 80.2 92.9 36.2 42.2 62.5 57.2 30.8 31 56.4 72 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(40:3) - 25.9 - 33.4 22.5 35.3 42.5 23.9 34.7 29.9 25.5 35.2 29.4 32.6 39 28.3 29.5 29.9 39.6 41.8 16.5 20.5 20.6 22 9.29 8.79 16 12.1 10.8 8.18 11.5 16.5 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(40:4) - 37.4 - 53.3 32.5 47 60.4 35.8 46.4 38.4 40.2 55.4 37.4 54.1 67.2 44.2 39.3 36.2 61.8 56.6 22.6 26.8 28.7 31.2 9.2 14.8 20.5 19.5 10.5 10.4 19.6 24 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(40:5) - 72.8 - 94.2 58.4 93.9 129 69.7 84.5 74.3 75.8 108 68.5 88 134 96.5 71.4 72.2 123 124 42.8 50.6 61.5 64.6 23.8 28.9 42.7 37.4 20 20 42 49.9 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(40:6) - 123 - 176 115 178 249 138 131 127 154 189 131 149 268 178 129 131 218 243 81.4 103 127 120 42.1 48.8 88.6 79.1 44.9 40.2 87.2 124 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(40:7) - 177 - 236 160 233 349 214 176 177 200 276 186 191 345 247 180 179 279 322 118 146 183 168 61.3 73.5 133 121 65.4 59 129 189 pmol/sample Ratio plot Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp - PG(42:10) - 30.5 - 36.9 27.3 39.5 46 28.8 30.2 29.3 29 39.1 39.9 32.2 53.2 37.6 46.3 41.6 37.8 57.9 31.4 34.1 37.5 23 11.4 15.3 19.3 18 15.3 13.3 12.9 24.5 pmol/sample