Microarray data for Acot1: fold-change ratios

Bone marrow-derived macrophage cells treated with Kdo2-Lipid A (KLA) and ATP

Gene Time course graph Description Treatment 15min 30min 1h 2h 4h 8h 20h
Acot1
KLA  ATP  ATP+KLA
 acyl-CoA thioesterase 1 (Acot1), mRNA [NM_012006] KLA .40 .34 .32 .29 .23 .17 .16
 ATP 1.01 .95 1.14 1.10 .64 .26 .53
 ATP+KLA .37 .37 .42 .42 .32 .20 .22

Bone marrow-derived macrophage cells treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Acot1
25OHC/Ctl - treatment with 25-OHC / no treatment
25-OHC*/* - lipid depleted media containing compactin and treatment with 25-OHC / lipid depleted media containing compactin only
acyl-CoA thioesterase 1 (Acot1), mRNA [NM_012006] 25OHC/Ctl (?) .99 .98 .99 1.02 1.12 1.26 1.20
 25-OHC*/* (?) .92 1.13 1.09 1.16 1.15 1.01 .90
 
 

Thioglycolate-elicited macrophage cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Acot1
KLA  Compactin  Compactin+KLA
 acyl-CoA thioesterase 1 KLA 1.03 .97 .80 .39 .29 .29 .31
 Compactin 1.00 .94 1.08 .98 .90 .91 .86
 Compactin+KLA 1.08 .91 .79 .41 .27 .25 .30
Acot1
KLA  Compactin  Compactin+KLA
 acyl-CoA thioesterase 1 (Acot1), mRNA [NM_012006] KLA 1.07 .92 .82 .41 .32 .27 .32
 Compactin .99 .97 1.03 .97 .92 .97 .86
 Compactin+KLA 1.01 .89 .77 .41 .29 .25 .28

RAW 264.7 cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Acot1
KLA  Compactin  Compactin+KLA
 acyl-CoA thioesterase 1 KLA .86 .91 .78 .50 .39 .39 .39
 Compactin .88 .99 1.01 1.01 .93 .86 .93
 Compactin+KLA .90 .86 .85 .51 .38 .38 .32
Acot1
KLA  Compactin  Compactin+KLA
 acyl-CoA thioesterase 1 (Acot1), mRNA [NM_012006] KLA .99 .93 .82 .49 .38 .39 .36
 Compactin .94 .93 1.00 1.05 .93 .83 .87
 Compactin+KLA .97 .82 .86 .53 .39 .38 .34