Microarray data for Hmgcs1: fold-change ratios

Bone marrow-derived macrophage cells treated with Kdo2-Lipid A (KLA) and ATP

Gene Time course graph Description Treatment 15min 30min 1h 2h 4h 8h 20h
Hmgcs1
KLA  ATP  ATP+KLA
15 days embryo male testis cDNA, RIKEN full-length enriched library, clone:8030402P03 product:similar to hydroxymethylglutaryl-CoA synthase (EC 4.1.3.5) (fragment) [Gallus gallus], full insert sequence. [AK078743] KLA 1.06 1.08 .98 .81 .88 .71 .86
 ATP 1.39 1.87 10.43 2.82 1.19 1.14 1.09
 ATP+KLA 1.36 1.59 5.22 1.83 1.21 .75 .66
Hmgcs1
KLA  ATP  ATP+KLA
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (Hmgcs1), mRNA [NM_145942] KLA 1.16 1.10 1.26 1.16 .88 .63 .69
 ATP 1.01 1.10 1.87 2.36 1.98 .89 .97
 ATP+KLA 1.12 1.20 1.33 1.69 1.39 .67 .51

Bone marrow-derived macrophage cells treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Hmgcs1
25OHC/Ctl - treatment with 25-OHC / no treatment
25-OHC*/* - lipid depleted media containing compactin and treatment with 25-OHC / lipid depleted media containing compactin only
15 days embryo male testis cDNA, RIKEN full-length enriched library, clone:8030402P03 product:similar to hydroxymethylglutaryl-CoA synthase (EC 4.1.3.5) (fragment) [Gallus gallus], full insert sequence. [AK078743] 25OHC/Ctl (?) .98 .60 .53 .64 .66 .58 .58
 25-OHC*/* (?) .77 .23 .24 .24 .24 .21 .22
 
 
Hmgcs1
25OHC/Ctl - treatment with 25-OHC / no treatment
25-OHC*/* - lipid depleted media containing compactin and treatment with 25-OHC / lipid depleted media containing compactin only
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (Hmgcs1), mRNA [NM_145942] 25OHC/Ctl (?) .96 .82 .47 .49 .48 .48 .41
 25-OHC*/* (?) .96 .79 .23 .14 .16 .16 .20
 
 

Thioglycolate-elicited macrophage cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Hmgcs1
KLA  Compactin  Compactin+KLA
15 days embryo male testis cDNA, RIKEN full-length enriched library, clone:8030402P03 product:similar to hydroxymethylglutaryl-CoA synthase (EC 4.1.3.5) (fragment) [Gallus gallus], full insert sequence. [AK078743] KLA 1.08 1.40 .90 .86 .85 .83 .88
 Compactin 1.00 1.25 .95 .97 1.00 .92 .95
 Compactin+KLA 1.22 1.16 .85 .96 .77 .86 .84
Hmgcs1
KLA  Compactin  Compactin+KLA
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (Hmgcs1), mRNA [NM_145942] KLA 1.05 1.18 .94 .91 .74 .62 .60
 Compactin .93 1.00 .99 .95 1.13 1.07 1.09
 Compactin+KLA .99 1.20 1.14 1.01 .86 .63 .67

RAW 264.7 cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Hmgcs1
KLA  Compactin  Compactin+KLA
15 days embryo male testis cDNA, RIKEN full-length enriched library, clone:8030402P03 product:similar to hydroxymethylglutaryl-CoA synthase (EC 4.1.3.5) (fragment) [Gallus gallus], full insert sequence. [AK078743] KLA 3.14 1.68 .79 .65 .96 1.08 1.26
 Compactin 1.10 1.04 1.11 1.06 1.12 .94 1.05
 Compactin+KLA 3.31 2.07 .95 .81 1.01 .87 .97
Hmgcs1
KLA  Compactin  Compactin+KLA
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (Hmgcs1), mRNA [NM_145942] KLA .99 1.51 1.63 .73 .93 .91 .74
 Compactin .96 .99 1.16 1.24 1.11 1.02 .92
 Compactin+KLA 1.16 1.35 1.88 .94 1.20 1.03 .81