Microarray data for Hmgcs2: fold-change ratios

Bone marrow-derived macrophage cells treated with Kdo2-Lipid A (KLA) and ATP

Gene Time course graph Description Treatment 15min 30min 1h 2h 4h 8h 20h
Hmgcs2
KLA  ATP  ATP+KLA
 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (Hmgcs2), nuclear gene encoding mitochondrial protein, mRNA [NM_008256] KLA .92 1.10 1.00 .99 1.08 1.06 1.14
 ATP .99 1.02 1.00 1.00 .98 1.08 .92
 ATP+KLA .99 1.00 1.00 1.03 1.28 1.06 1.00

Bone marrow-derived macrophage cells treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Hmgcs2
25OHC/Ctl - treatment with 25-OHC / no treatment
25-OHC*/* - lipid depleted media containing compactin and treatment with 25-OHC / lipid depleted media containing compactin only
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (Hmgcs2), nuclear gene encoding mitochondrial protein, mRNA [NM_008256] 25OHC/Ctl (?) .97 .93 1.02 1.03 .98 1.00 1.03
 25-OHC*/* (?) .96 1.05 .98 .95 .97 1.00 .99
 
 

Thioglycolate-elicited macrophage cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Hmgcs2
KLA  Compactin  Compactin+KLA
 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (Hmgcs2), nuclear gene encoding mitochondrial protein, mRNA [NM_008256] KLA 1.10 .98 1.06 1.08 1.00 1.00 1.05
 Compactin 1.03 1.11 .97 1.00 1.08 1.01 .93
 Compactin+KLA 1.00 .96 .97 1.01 1.03 .98 .96

RAW 264.7 cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Hmgcs2
KLA  Compactin  Compactin+KLA
 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (Hmgcs2), nuclear gene encoding mitochondrial protein, mRNA [NM_008256] KLA 1.01 1.03 .95 1.02 1.04 1.01 .96
 Compactin .99 .99 1.03 .98 1.09 1.04 .98
 Compactin+KLA 1.03 1.04 1.04 .95 1.00 1.02 .98