Microarray data for Lass6: fold-change ratios

Bone marrow-derived macrophage cells treated with Kdo2-Lipid A (KLA) and ATP

Gene Time course graph Description Treatment 15min 30min 1h 2h 4h 8h 20h
Lass6
KLA  ATP  ATP+KLA
 adult male colon cDNA, RIKEN full-length enriched library, clone:9030601N10 product:unclassifiable, full insert sequence [AK136463] KLA 6.80 6.12 6.10 6.59 4.37 2.19 3.16
 ATP 1.11 1.03 1.26 1.13 2.24 2.53 .84
 ATP+KLA 6.23 6.58 7.50 5.95 5.29 2.64 1.33
Lass6
KLA  ATP  ATP+KLA
 LAG1 homolog, ceramide synthase 6 (Lass6), mRNA [NM_172856] KLA 8.82 11.21 11.49 12.30 10.83 7.97 7.15
 ATP 1.11 1.13 1.22 1.58 2.13 4.41 4.45
 ATP+KLA 10.82 12.09 9.72 11.37 7.17 5.10 9.51

Bone marrow-derived macrophage cells treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Lass6
25OHC/Ctl - treatment with 25-OHC / no treatment
25-OHC*/* - lipid depleted media containing compactin and treatment with 25-OHC / lipid depleted media containing compactin only
adult male colon cDNA, RIKEN full-length enriched library, clone:9030601N10 product:unclassifiable, full insert sequence [AK136463] 25OHC/Ctl (?) 1.00 1.03 .97 1.03 .92 .95 .91
 25-OHC*/* (?) 1.05 1.01 .93 1.08 .79 .77 .70
 
 
Lass6
25OHC/Ctl - treatment with 25-OHC / no treatment
25-OHC*/* - lipid depleted media containing compactin and treatment with 25-OHC / lipid depleted media containing compactin only
LAG1 homolog, ceramide synthase 6 (Lass6), mRNA [NM_172856] 25OHC/Ctl (?) 1.07 1.04 1.17 .85 1.02 1.06 1.10
 25-OHC*/* (?) 1.21 1.02 1.18 .92 .96 .84 .80
 
 

Thioglycolate-elicited macrophage cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Lass6
KLA  Compactin  Compactin+KLA
 LAG1 homolog, ceramide synthase 6 (Lass6), mRNA [NM_172856] KLA 1.06 1.64 2.96 7.50 7.56 6.60 3.62
 Compactin 1.03 1.00 .99 .97 1.10 1.04 1.01
 Compactin+KLA 1.05 1.56 2.83 7.79 7.97 5.53 3.14
Lass6
KLA  Compactin  Compactin+KLA
 adult male colon cDNA, RIKEN full-length enriched library, clone:9030601N10 product:unclassifiable, full insert sequence [AK136463] KLA 1.06 .85 1.48 3.63 2.38 1.53 1.35
 Compactin 1.04 .98 .90 .82 1.07 1.03 .86
 Compactin+KLA .95 1.02 1.87 2.68 1.82 1.66 1.51

RAW 264.7 cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Lass6
KLA  Compactin  Compactin+KLA
 adult male colon cDNA, RIKEN full-length enriched library, clone:9030601N10 product:unclassifiable, full insert sequence [AK136463] KLA .98 .88 1.08 1.52 .86 .76 .73
 Compactin .99 .97 .82 .79 1.09 .87 1.01
 Compactin+KLA .90 1.13 .87 1.43 1.08 1.09 1.03
Lass6
KLA  Compactin  Compactin+KLA
 LAG1 homolog, ceramide synthase 6 (Lass6), mRNA [NM_172856] KLA 1.14 1.29 1.45 1.99 1.93 1.51 1.19
 Compactin 1.09 1.04 .93 1.04 .99 .98 1.18
 Compactin+KLA 1.18 1.22 1.31 2.16 2.18 1.48 1.33