Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: 24-dihydro Lanosterol Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 0.370.32 0.287 0.34 0.355 0.277 0.233 0.233 1.04 1.52 3.4 1.31 1.14 1.36 0.95 0.757 0.819 1.34 1.31 0.908 0.923 1.04 2.22 1.06 0.959 0.851 1.56 1.65 0.989 1.17 1.06 1 1.17 0.7 0.527 1.41 1.35 0.808 - 0.642 0.945 - 0.63 0.712 1.09 - - - 0.584 0.957 - - - 0.432 - - 55.6 65.6 - 29.6 - - 18 - - - - - 34.3 - 86.4 47.4 - 99.9 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010087 24-dihydro Lanosterol - 0.09 - 0.09 0.06 0.06 0.08 0.07 0.05 0.05 0.07 0.06 0.08 0.06 0.05 0.07 0.06 0.12 0.06 0.09 0.03 0.04 0.04 0.07 - 0.06 0.04 0.06 0.02 0.06 0.02 0.07 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010087 24-dihydro Lanosterol - 0.41 - 0.51 0.45 0.56 0.67 0.49 0.47 0.51 0.49 0.5 0.38 0.51 0.59 0.51 0.4 0.43 0.67 0.7 0.4 0.43 0.32 0.71 0.44 0.36 0.42 0.41 0.37 0.32 0.36 0.59 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010087 24-dihydro Lanosterol - 0.88 - 0.92 0.68 0.62 0.9 0.47 0.61 0.59 0.75 0.67 0.63 0.53 0.72 0.86 0.43 0.99 0.47 0.82 0.33 0.45 0.54 1.06 - 0.5 0.55 0.9 0.24 0.51 0.2 0.79 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010087 24-dihydro Lanosterol - 4.01 - 5.22 5.08 5.82 7.53 3.27 5.76 6.03 5.22 5.57 3.01 4.53 8.48 6.24 2.87 3.54 5.2 6.34 4.35 4.8 4.35 10.8 4.35 2.99 5.78 6.15 4.36 2.74 3.66 6.62 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMST01010087 24-dihydro Lanosterol umol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMST01010087 24-dihydro Lanosterol umol/ml of media Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120120) LMST01010087 24-dihydro Lanosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120131) LMST01010087 24-dihydro Lanosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120212) LMST01010087 24-dihydro Lanosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120308) LMST01010087 24-dihydro Lanosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0002) LMST01010087 24-dihydro Lanosterol pmol/mg Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0014) LMST01010087 24-dihydro Lanosterol pmol/ml