Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: 24S-hydroxy-cholesterol

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
0.5470.493 0.547 0.67 4.46 4.68 4.37 3.38 0.318 0.69 1.17 0.671 0.659 0.84 1.04 0.84 0.746 0.953 1.26 0.746 0.556 0.82 0.614 0.525 0.692 0.786 0.518 0.352 0.482 0.298 0.284 0.435 0.789 0.541 0.253 0.409 0.521 0.854 0.174 0.186 0.9 0.243 0.534 0.51 1.47 0.339 0.137 0.385 0.373 0.36 0.635 0.117 0.593 0.443 0.562 0.723 95.9 173 178 72.7 57.2 143 91.5 158 165 97.2 170 115 120 55.2 37.1 49.6 148 124
Details Graph
Individual
Expts
Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
0 hrs
25-OHC*
*
25-OHC
Ctrl
30 min
25-OHC*
*
25-OHC
Ctrl
1 hr
25-OHC*
*
25-OHC
Ctrl
2 hrs
25-OHC*
*
25-OHC
Ctrl
4 hrs
25-OHC*
*
25-OHC
Ctrl
8 hrs
25-OHC*
*
25-OHC
Ctrl
12 hrs
25-OHC*
*
25-OHC
Ctrl
24 hrs
Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)
LMST01010019 24S-hydroxy-cholesterol -
0.05
-
0.07
0.03
0.08
0.02
0.04
0.03
0.06
0.03
0.06
0.17
0.1
0.04
0.07
0.19
0.11
0.03
0.05
0.07
0.09
0.04
0.06
0.05
0.09
0.03
0.07
0.17
0.1
0.17
0.08
pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)
LMST01010019 24S-hydroxy-cholesterol -
0.1
-
0.06
0.17
0.1
0.18
0.04
0.15
0.09
0.19
0.07
0.22
0.09
0.13
0.05
0.24
0.12
0.19
0.08
0.22
0.11
0.11
0.09
0.26
0.09
0.1
0.05
0.18
0.08
0.15
0.07
pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)
LMST01010019 24S-hydroxy-cholesterol -
0.06
-
0.03
0.04
0.03
0.19
0.03
0.04
0.04
0.05
0.08
0.04
0.04
0.06
0.09
0.06
0.03
0.09
0.18
0.03
0.06
0.07
0.09
0.06
0.06
0.07
0.05
0.07
0.06
0.08
0.09
pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)
LMST01010019 24S-hydroxy-cholesterol -
0.49
-
0.72
0.34
0.83
0.22
0.27
0.37
0.71
0.32
0.67
1.34
0.89
0.57
0.86
1.36
0.91
0.23
0.45
0.76
1
0.54
0.91
0.49
0.75
0.41
1.05
2
0.86
1.73
0.9
pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)
LMST01010019 24S-hydroxy-cholesterol -
0.98
-
0.61
1.92
1.04
2.02
0.27
1.84
1.06
2.02
0.78
1.74
0.8
1.87
0.61
1.72
0.99
1.47
0.72
2.39
1.23
1.5
1.36
2.57
0.75
1.38
0.75
2.12
0.68
1.52
0.79
pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)
LMST01010019 24S-hydroxy-cholesterol -
0.59
-
0.31
0.45
0.31
2.13
0.2
0.49
0.47
0.53
0.89
0.32
0.36
0.86
1.1
0.43
0.25
0.7
1.63
0.33
0.67
0.95
1.36
0.59
0.5
0.96
0.75
0.82
0.51
0.81
1.01
pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ110501)
LMST01010019 24S-hydroxy-cholesterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)
LMST01010019 24S-hydroxy-cholesterol umol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)
LMST01010019 24S-hydroxy-cholesterol umol/ml of media
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120120)
LMST01010019 24S-hydroxy-cholesterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120131)
LMST01010019 24S-hydroxy-cholesterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120212)
LMST01010019 24S-hydroxy-cholesterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120308)
LMST01010019 24S-hydroxy-cholesterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0016)
LMST01010019 24S-hydroxy-cholesterol pmol/mg
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0015)
LMST01010019 24S-hydroxy-cholesterol pmol/ml