Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: 25-hydroxy-cholesterol Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 -- - - 0.08 0.097 0.103 0.12 0.247 0.13 0.177 0.2 0.215 0.167 0.153 0.127 - - - - - - 0.205 0.17 2.42 2.92 2.97 2.39 2.43 2.26 2.65 3.53 2.94 2.96 3.48 3.89 3.87 3.91 8.76 5.06 1.33 1.67 1.59 1.51 2.02 1.74 1.91 2.52 0.1 0.18 0.16 0.09 0.11 0.08 0.08 0.15 0.13 0.12 0.1 0.09 0.09 0.11 0.08 0.13 0.11 0.08 0.08 0.14 0.08 0.08 0.08 0.12 - - - 0.046 0.119 0.083 - - 0.15 0.085 0.106 - - 0.073 0.063 0.105 - - 0.075 0.057 0.074 0.063 0.076 0.088 3.23 3.93 2.57 3.77 2.04 2.08 2.72 3.16 2.66 2.93 2.44 2.24 2.51 2.67 3.22 3.76 3.31 3.67 2.04 3.65 2.99 3.24 3.12 2.54 3.95 3.03 2.56 2.99 2.55 2.66 3.08 3.18 3.14 2.07 2.14 1.81 1.8 2.02 2.2 2.35 2.71 2.09 1.87 1.98 1.91 2.32 2.28 2.12 - 0.1 0.127 0.267 0.283 0.47 - - - 0.037 0.033 0.036 0.034 0.037 0.062 0.059 0.067 0.333 - 0.025 0.045 0.045 0.045 0.051 0.071 0.054 0.05 0.052 0.042 0.047 0.051 0.057 0.091 0.278 0.244 0.255 0.215 0.205 0.255 0.186 0.211 - - 0.051 5.73 5.41 - 6.39 7.61 3.63 5.16 4.81 5.04 5.12 20.3 17.5 17.5 18.9 0.221 0.249 0.842 0.323 0.174 0.402 0.222 0.221 0.249 0.166 0.268 0.221 0.13 0.137 0.19 0.249 0.231 0.144 0.476 0.683 0.292 0.236 0.237 0.311 0.303 0.256 0.281 0.116 0.164 0.136 0.083 0.072 0.064 0.151 0.083 0.058 0.039 0.106 0.106 0.128 0.091 0.166 0.091 0.039 0.061 0.058 0.064 0.128 17.5 21.3 35.3 9.4 12.6 19.3 14.4 21.3 10.2 25.1 23.9 9.69 22.6 21.1 15.2 25.8 15.7 25.1 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070605) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070612) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070820) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ071011) LMST01010018 25-hydroxy-cholesterol pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070726) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070808) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070823) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080212) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080219) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080226) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD080211) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD080311) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD080408) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010018 25-hydroxy-cholesterol - 1.07 - 1.51 53.9 0.96 120 1.92 133 0.91 149 1.16 156 1.27 111 1.29 280 1.41 269 1.65 169 1.19 171 1.32 184 1.94 224 1.53 170 1.87 257 1.93 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010018 25-hydroxy-cholesterol - 1.35 - 1.73 76.4 1.01 83.7 1.21 133 0.68 144 1.36 208 0.82 134 1.5 275 1.52 206 2.38 210 1.09 153 1.89 285 1.22 176 1.66 234 1.36 159 2.46 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010018 25-hydroxy-cholesterol - 9.74 - 11.1 208 11.2 317 9.68 270 10.7 330 11.2 313 10 397 8.59 381 10.4 598 14.9 412 13.2 385 13.9 582 15.4 587 11.6 586 19.6 585 25.8 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010018 25-hydroxy-cholesterol - 10.5 - 15.5 609 9.98 1350 12.8 1625 10.8 1591 12.9 1230 11.3 1593 15.8 2011 11.6 2085 14.9 1844 13.3 2324 20 1814 16.1 3082 23 2001 16 2610 21.7 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010018 25-hydroxy-cholesterol - 13.2 - 17.7 863 10.5 941 8.07 1630 8.04 1536 15.2 1647 7.28 1928 18.4 1976 12.5 1600 21.6 2280 12.2 2084 28.6 2816 10.1 2423 24.9 2751 11.6 1614 27.6 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010018 25-hydroxy-cholesterol - 95.3 - 113 2346 117 3564 64.6 3310 127 3515 124 2476 88.8 5709 105 2735 85.9 4639 135 4487 147 5231 210 5755 128 8074 174 6902 168 5945 290 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070619) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070724) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ060926) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ061017) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ061024) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ061024) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ061010) LMST01010018 25-hydroxy-cholesterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070717) LMST01010018 25-hydroxy-cholesterol pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070927) LMST01010018 25-hydroxy-cholesterol pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070927) LMST01010018 25-hydroxy-cholesterol pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070927) LMST01010018 25-hydroxy-cholesterol pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJSUB000) LMST01010018 25-hydroxy-cholesterol pmol/mg protein Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB300) 25-hydroxy-cholesterol pmol/mg protein Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMST01010018 25-hydroxy-cholesterol umol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMST01010018 25-hydroxy-cholesterol umol/ml of media Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120120) LMST01010018 25-hydroxy-cholesterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120131) LMST01010018 25-hydroxy-cholesterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120212) LMST01010018 25-hydroxy-cholesterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120308) LMST01010018 25-hydroxy-cholesterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0008) LMST01010018 25-hydroxy-cholesterol pmol/mg Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0003) LMST01010018 25-hydroxy-cholesterol pmol/ml