Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: 3beta,5alpha,6beta-trihydroxycholestanol

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
-- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010052 3beta,5alpha,6beta-trihydroxycholestanol -
0.08
-
-		 0.09
0.05
0.06
0.02		 0.02
0.13
0.1
0.08		 0.18
0.06
0.04
0.06		 0.28
0.35
0.04
0.18		 0.12
0.05
0.05
0.01		 -
0.07
0.13
0.06		 -
-
0.01
0.4		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010052 3beta,5alpha,6beta-trihydroxycholestanol -
0.22
-
0.35		 0.16
0.17
0.29
0.31		 0.13
0.08
0.31
0.19		 0.16
0.09
0.19
0.26		 0.2
0.06
0.38
0.37		 0.14
0.08
0.31
0.25		 0.11
0.12
0.3
0.16		 0.1
0.11
0.32
0.17		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010052 3beta,5alpha,6beta-trihydroxycholestanol -
0.14
-
0.63		 0.18
0.28
0.59
0.65		 0.15
0.16
0.45
0.47		 0.23
0.19
0.63
0.58		 0.15
0.12
1.32
0.81		 0.43
0.51
0.54
0.71		 0.53
0.53
1.73
0.89		 0.71
1.48
2.4
1.08		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010052 3beta,5alpha,6beta-trihydroxycholestanol -
0.78
-
-		 1.02
0.52
0.67
0.13		 0.25
1.54
1.07
0.89		 1.42
0.53
0.57
0.73		 2.01
2.88
0.31
1.63		 1.31
0.56
0.68
0.15		 -
0.58
1.79
0.9		 -
-
0.1
4.49		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010052 3beta,5alpha,6beta-trihydroxycholestanol -
2.15
-
3.58		 1.81
1.77
3.26
2.07		 1.59
0.95
3.3
2.12		 1.27
0.8
2.73
3.18		 1.43
0.49
2.95
3.35		 1.52
0.89
4.22
3.78		 1.09
1
4.13
2.4		 1.18
0.94
3.25
1.91		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010052 3beta,5alpha,6beta-trihydroxycholestanol -
1.37
-
6.44		 2.03
2.91
6.63
4.34		 1.84
1.89
4.8
5.24		 1.82
1.69
9.05
7.1		 1.08
0.99
10.2
7.34		 4.68
5.69
7.34
10.8		 5.24
4.4
23.8
13.4		 8.36
12.7
24.4
12.1		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0009)		LMST01010052 3beta,5alpha,6beta-trihydroxycholestanol pmol/mg
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0012)		LMST01010052 3beta,5alpha,6beta-trihydroxycholestanol pmol/ml