Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: 6alpha-hydroxy-cholestanol

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
270226 180 146 118 160 1.45 1.69 1.52 1.18 1.69 1.8 0.852 1.06 0.989 1.86 1.72 0.87 0.947 0.944 1.04 1.12 1.07 0.938 1.99 2.01 1.17 1.12 1.54 1.43 1.16 0.651 0.713 1.27 1.38 0.911 0.163 - 0.132 - 0.025 0.05 - 0.232 0.05 0.037 0.253 - 0.228 0.125 0.062 0.062 0.05 0.037 11.9 9.94 2.45 11.9 4.03 4.72 12.4 12.6 5.38 3.23 13.1 6.21 7.32 20.6 4.97 5.47 4.97 2.96
Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010135 6alpha-hydroxy-cholestanol -
0.85
-
0.98		 0.71
0.77
0.91
1.62		 0.66
0.69
0.91
0.83		 1.08
1
0.66
0.77		 1.17
0.9
1.04
1.05		 0.58
0.77
0.65
0.69		 0.71
1.09
0.67
0.73		 0.42
0.48
0.47
1.01		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010135 6alpha-hydroxy-cholestanol -
0.67
-
0.94		 0.9
0.82
1.09
0.85		 0.78
0.59
0.76
0.74		 0.91
0.57
0.75
0.69		 0.93
1.23
0.93
1.35		 0.6
0.59
0.76
0.84		 0.74
0.71
0.93
0.74		 0.71
0.58
0.93
0.9		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010135 6alpha-hydroxy-cholestanol -
0.75
-
1.62		 0.94
1.01
2.11
1.4		 0.98
1.05
1.87
1.84		 0.9
1
1.43
0.92		 1.04
0.7
2.95
2.5		 0.92
0.89
1.59
2.04		 1.03
1.39
1.35
1.47		 1.06
1.05
2.42
2.45		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010135 6alpha-hydroxy-cholestanol -
8.32
-
10		 8.02
8
10.2
10.8		 8.09
8.16
9.7
9.25		 8.54
8.88
9.48
9.43		 8.39
7.42
8.07
9.51		 6.31
8.59
8.84
10.4		 7.02
9.04
9.22
11		 4.95
4.11
4.77
11.3		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010135 6alpha-hydroxy-cholestanol -
6.56
-
9.62		 10.2
8.52
12.2
5.67		 9.57
6.98
8.1
8.25		 7.2
5.06
10.8
8.45		 6.67
10.1
7.21
12.2		 6.53
6.58
10.3
12.7		 7.31
5.89
12.8
11.1		 8.36
4.96
9.45
10.1		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010135 6alpha-hydroxy-cholestanol -
7.34
-
16.6		 10.6
10.5
23.7
9.34		 12
12.4
19.9
20.5		 7.12
8.88
20.5
11.3		 7.46
5.77
22.9
22.6		 10
9.93
21.6
30.9		 10.2
11.5
18.6
22.1		 12.5
8.98
24.6
27.5		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJSUB001)		LMST01010135 6alpha-hydroxy-cholestanol pmol/mg protein
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCISUB300)		 6alpha-hydroxy-cholestanol pmol/mg protein
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120120)		LMST01010135 6alpha-hydroxy-cholestanol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120131)		LMST01010135 6alpha-hydroxy-cholestanol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120212)		LMST01010135 6alpha-hydroxy-cholestanol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120308)		LMST01010135 6alpha-hydroxy-cholestanol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0006)		LMST01010135 6alpha-hydroxy-cholestanol pmol/mg
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0011)		LMST01010135 6alpha-hydroxy-cholestanol pmol/ml