Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: Arachidonic acid Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 25.114.4 12.9 14.7 7.1 3.11 3.67 1.44 14.8 3.84 1.01 55.7 4.9 1.56 31.9 2.58 0.737 3.03 1.55 1.14 9.49 3.81 0.783 19.2 17.2 22.9 21 10.2 5.24 4.37 3.83 2.42 3.21 2.45 0.935 0.953 2.07 6.49 3.1 2.92 2.83 2.22 1.06 1.35 1.24 3.78 5.78 4.46 3.61 3.83 1.99 2.38 2.2 7.65 10 10.4 8.09 7.46 7.51 6.32 10.5 12.9 11.3 13.6 14.5 12.2 10.9 8.91 10.6 7.45 8.57 7.68 8.92 8.45 6.52 6.33 5.39 4.29 8.91 8.75 7.92 5.95 6.08 7.29 14.1 8.86 7.63 8.58 7.45 9.63 8.64 10.5 11.7 5.68 6.47 8.09 8.89 8.97 14.9 21.3 29 223 183 180 149 130 117 136 145 276 262 179 151 152 59.5 60.1 71.1 232 210 224 163 163 105 121 131 177 152 108 181 82.9 74.1 72.1 246 54.6 85.9 53.1 79.4 69.7 59.8 43.2 54.1 56.8 56.8 60.3 54.3 46.7 42.1 32.3 85.1 9.57 15.3 10.4 13.9 7.6 7.12 7.77 9.45 7.87 11.4 9.89 8.25 9.33 9.15 9.21 11.2 7.04 6.32 5.6 6.3 7.4 7.32 6.51 5.93 8.41 5.21 7.01 5.16 6.32 6.01 6.43 7.41 6.95 6.97 6.99 5.87 6.7 4.96 6.78 9.29 6 7.01 6.14 6.44 7.12 5.72 7.02 8.37 264 228 106 12.6 3.21 2.96 94.6 91.3 88.1 92.8 61.5 35.5 18.4 4.65 62.6 62.6 68.2 59.3 48.8 27.9 14.3 3.84 85 66.4 62.5 54.2 36.8 17.7 9.48 3.19 6.87 8.08 7.8 6.44 4.4 1.71 1.19 0.633 3.42 2.74 6.21 3.22 2.22 1.38 0.917 0.41 15.7 22.2 18.9 14.5 15.1 9.06 10.3 8.93 10.3 5.37 19.4 4.46 3.92 4.93 5.64 5.55 179 220 195 158 163 219 144 105 185 191 187 150 114 137 157 160 141 129 173 254 176 142 181 147 187 123 78.8 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ060404) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070710) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070804) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070612) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070804) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070814) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070817) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080227) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080325) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080325) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080227) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080520) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080520) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080702) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080702) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080702) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD080211) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD080311) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD080408) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090908) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090909) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090909) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090924) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090924) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMFA01030001 Arachidonic acid - 6.6 - 14.4 6.31 7.25 14.1 25.1 5.33 5.97 14.3 13.3 7.33 7.23 10.8 12.5 9.05 7.17 16 14.1 5.53 5.03 9.13 7.94 5.18 5 7.75 7.32 2.76 6.57 10.8 12 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMFA01030001 Arachidonic acid - 10.6 - 18.4 9.1 7.26 23.3 13.7 8.22 7.06 17.2 15.3 8.06 5.57 11.3 14.5 6.37 9.18 16.7 19 3.91 4.94 9.71 11.7 4.37 3.82 9.74 9.26 7.32 4.1 9.67 17.8 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMFA01030001 Arachidonic acid - 8.53 - 10 9.27 7.45 5.36 14.8 7.42 7.15 6.36 8.6 7.17 10.6 5.03 6.15 11.2 10.8 11.7 9.67 7.82 7.7 7.45 7.29 11.2 10.8 6.62 10.8 9.92 15.6 10.9 12.2 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMFA01030001 Arachidonic acid - 12.5 - 8.43 6.43 6.03 7.95 7.14 5.99 7.85 8.37 5.99 5.84 5.12 4.12 5.79 9.62 8.9 6.87 10.8 6.16 7 7.88 10.8 8.1 7.68 6.64 10.2 9.54 12.6 8.37 9.63 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMFA01030001 Arachidonic acid - 12.8 - 14.8 14.3 18.3 25.4 17.9 11.7 18 24.9 19.4 14.8 13.6 23.1 22 12.6 17.4 34.6 31.3 17.3 18.9 24.4 30 24 29.8 30.8 25.1 21.4 22.8 32.1 38.8 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMFA01030001 Arachidonic acid - 64.6 - 147 71.3 75.3 158 167 65.4 70.6 152 149 58 64.2 156 153 64.9 59.1 124 128 60.2 56.1 124 120 51.2 41.5 107 110 32.5 56.2 109 135 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMFA01030001 Arachidonic acid - 78.9 - 173 75.2 64.3 207 149 76.1 70.3 171 178 61.9 62.2 138 157 49.4 60.6 156 135 39.3 52.8 116 121 36.9 36.9 104 128 72.9 38 104 179 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMFA01030001 Arachidonic acid - 83.5 - 103 105 77.4 60.2 98.5 91 84.6 67.8 95.8 56.7 94.3 72.3 75.3 80.6 89.3 91 87.6 85.1 85.9 101 110 110 89.6 91.1 162 117 134 111 137 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMFA01030001 Arachidonic acid - 92.7 - 79.3 53.1 53.4 70.6 77.9 55.5 78.2 83.1 69.5 44.9 57.2 50.4 62.7 74.6 58.8 64.2 76.6 61.9 74.8 94.1 112 68.3 74.3 71.2 142 95 117 89.8 96.8 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMFA01030001 Arachidonic acid - 103 - 110 104 106 150 127 85.7 112 162 142 103 89.7 131 172 86.3 118 155 148 117 126 184 168 124 136 165 178 114 111 149 200 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120308) LMFA01030001 Arachidonic acid pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (ELG050520) LMFA01030001 Arachidonic acid ng/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG060127) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG060201) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG060210) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070831) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070927) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070830) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070924) LMFA01030001 Arachidonic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070122) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070123) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070124) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070907) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070914) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070919) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070815) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070316) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070316) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070316) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070717) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070815) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070716) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070816) LMFA01030001 Arachidonic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120120) LMFA01030001 Arachidonic acid pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120131) LMFA01030001 Arachidonic acid pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120212) LMFA01030001 Arachidonic acid pmol/10E6 cells