Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: C14DH Sphingomyelin

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
0.811.07 1.1 0.81 0.78 0.69 0.65 0.58 1.15 1.06 1.07 1.12 0.82 0.72 0.62 0.57 0.69 0.75 0.64 0.7 0.65 0.57 0.3 0.31 0.28 0.21 0.2 0.29 0.33 0.25 0.28 0.34 0.16 0.32 0.25 0.23 0.23 0.29 0.27 0.42 0.27 0.36 0.3 0.31 0.24 0.21 0.39 0.27 0.764 0.969 0.555 0.893 0.452 0.449 0.506 0.745 4.78 5.51 4.01 4.04 4.22 4.39 4.56 6.75 0.003 0.012 0.012 0.009 0.003 0.012 0.01 0.009 0.382 0.485 0.277 0.447 0.226 0.224 0.253 0.373 0.314 0.362 0.264 0.266 0.278 0.288 0.3 0.443 0.168 0.144 0.148 0.247 0.185 0.163 0.17 0.085 0.201 0.119 0.185 0.202 0.158 0.134 0.168 0.107 0.188 0.141 0.213 0.114 0.149 0.111 0.128 0.106 0.163 0.142 0.187 0.125 0.158 0.128 0.132 0.096 1.26 3.05 3.26 3.4 2.98 2.93 2.99 2.63 1.87 2.35 5.34 5.21 3.79 3.26 3.74 4.24 4.17 3.61 2.8 4.53 4.71 3.23 2.55 2.79 3.26 3.32 2.04 1.65 3.75 3.99 2.07 0.794 1.06 2.65 1.04 1.28 1.51 1.55 2.38 1.8 2.19 321 301 403 232 209 400 463 430 359 330
Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080212)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080219)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080226)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090127)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090114)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090401)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP03010030 C14DH Sphingomyelin pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ081209)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMSP03010030 C14DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMSP03010030 C14DH Sphingomyelin -
0.21
-
0.31		 0.22
0.26
0.22
0.32		 0.18
0.16
0.23
0.22		 0.3
0.27
0.17
0.22		 0.36
0.3
0.35
0.3		 0.21
0.22
0.22
0.21		 0.25
0.28
0.13
0.21		 0.14
0.22
0.22
0.21		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		LMSP03010030 C14DH Sphingomyelin -
0.17
-
0.2		 0.3
0.25
0.28
0.18		 0.33
0.32
0.27
0.24		 0.39
0.3
0.25
0.3		 0.4
0.51
0.31
0.42		 0.3
0.3
0.25
-		 0.33
0.32
0.29
0.23		 0.17
0.26
0.18
0.32		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		LMSP03010030 C14DH Sphingomyelin -
0.29
-
0.45		 0.29
0.39
0.57
0.44		 0.29
0.35
0.6
0.47		 0.34
0.33
0.5
0.43		 0.34
0.34
0.89
0.74		 0.3
0.35
0.45
0.7		 0.36
0.51
0.58
0.53		 0.22
0.37
0.51
0.65		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMSP03010030 C14DH Sphingomyelin -
2.05
-
3.17		 2.48
2.7
2.47
2.13		 2.21
1.89
2.45
2.45		 2.37
2.4
2.44
2.69		 2.58
2.47
2.71
2.72		 2.29
2.45
2.99
3.18		 2.47
2.32
1.79
3.15		 1.65
1.88
2.23
2.36		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		LMSP03010030 C14DH Sphingomyelin -
1.26
-
1.88		 2.48
2.21
2.48
1.96		 3.06
3.19
2.68
2.79		 3
3.35
3.06
3.25		 3.1
3.37
2.9
2.98		 3.01
3.2
2.98
-		 2.78
3.09
3.11
3.19		 1.69
2.41
1.93
3.22		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		LMSP03010030 C14DH Sphingomyelin -
2.32
-
3.36		 2.11
2.25
3.38
3.13		 2.13
2.18
3.9
3.43		 2.37
2.18
2.85
3.36		 2.33
2.32
3.99
3.49		 2.03
2.34
3.4
3.92		 1.86
2.34
3.12
3.75		 1.18
1.81
2.36
3.36		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		 C14DH Sphingomyelin pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120120)		LMSP03010030 C14DH Sphingomyelin pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120131)		LMSP03010030 C14DH Sphingomyelin pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120131)		LMSP03010030 C14DH Sphingomyelin pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120308)		LMSP03010030 C14DH Sphingomyelin pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0010)		LMSP03010030 C14DH Sphingomyelin pmol/mg
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0005)		LMSP03010030 C14DH Sphingomyelin pmol/ml