Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: C14 GlcCer

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
0.070.17 0.1 0.09 - - - 0.01 0.18 0.04 0.37 - 0.14 - - 0.03 0.17 0.04 0.03 0.14 0.09 - 0.02 0.08 0.03 0.03 0.02 0.03 0.02 0.02 0.03 0.03 0.02 0.01 0.01 0.02 0.01 0.02 0.04 0.05 0.07 0.06 0.04 0.03 0.05 0.02 0.01 0.01 0.018 - 0.016 - - - - 0.009 0.116 - 0.117 - - - - 0.077 0.048 0.018 0.037 - 0.014 0.042 0.02 0.021 0.009 - 0.008 - - - - 0.004 0.008 - 0.008 - - - - 0.005 0.069 - - 0.09 - - - 0.062 0.082 - - 0.074 - - - 0.078 0.01 0.014 0.025 0.014 0.013 0.002 0.008 0.016 0.009 0.014 0.022 0.016 0.014 0.003 0.008 0.015 0.077
Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080212)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080219)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080226)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090127)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090114)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090401)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP0501AA26 C14 GlcCer pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ081209)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMSP0501AA26 C14 GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMSP0501AA26 C14 GlcCer -
-
-
0.01		 0.01
0.02
0.01
0.01		 0.02
-
0.01
0.01		 0.01
0.01
-
0.01		 0.02
0.01
0.01
0.01		 0.01
0.01
-
0.01		 0.01
0.01
0.01
0.01		 0.01
0.01
0.01
0.01		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		LMSP0501AA26 C14 GlcCer -
0.02
-
0.01		 0.02
0.01
0.03
0.01		 0.02
0.02
0.01
0.01		 0.03
0.01
0.01
0.01		 0.01
0.02
0.02
0.02		 0.01
0.01
0.01
0.03		 0.01
0.01
0.01
0.01		 0.01
0.02
0.01
0.02		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		LMSP0501AA26 C14 GlcCer -
0.02
-
0.02		 0.02
0.02
0.03
0.03		 0.02
0.02
0.04
0.02		 0.03
0.02
0.02
0.02		 0.02
0.03
0.04
0.02		 0.02
0.03
0.02
0.04		 0.02
0.04
0.03
0.03		 0.01
0.03
0.02
0.04		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMSP0501AA26 C14 GlcCer -
-
-
0.1		 0.11
0.21
0.11
0.07		 0.25
-
0.11
0.11		 0.08
0.09
-
0.12		 0.14
0.08
0.08
0.09		 0.11
0.11
-
0.15		 0.1
0.08
0.14
0.15		 0.12
0.09
0.1
0.11		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		LMSP0501AA26 C14 GlcCer -
0.15
-
0.09		 0.17
0.09
0.27
0.11		 0.19
0.2
0.1
0.12		 0.23
0.11
0.12
0.11		 0.08
0.13
0.19
0.14		 0.1
0.11
0.12
0.31		 0.08
0.1
0.11
0.14		 0.1
0.19
0.11
0.2		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		LMSP0501AA26 C14 GlcCer -
0.16
-
0.15		 0.15
0.12
0.18
0.21		 0.15
0.12
0.26
0.15		 0.21
0.13
0.11
0.16		 0.14
0.2
0.18
0.09		 0.14
0.2
0.15
0.22		 0.1
0.18
0.16
0.21		 0.05
0.15
0.09
0.21		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		 C14 GlcCer pmol/10E6 cells