Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: C16 Sphingomyelin Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 24122127330631028634033633735963455158050853653452628828331327829631332612591.3 93.3 367 357 337 279 189 180 65.1 36.1 20 5996 179 202 217 167 163 151 140 132 196 192 189 202 163 172 142 150 144 134 135 160 138 130 99.5 102 321 293 267 307 297 257 284 282 332 374 290 326 266 306 258 297 476 464 466 441 327 321 402 276 364 460 251 458 250 224 279 366 2276 2619 1813 2077 2334 2189 2519 3316 131 91.5 109 92.2 102 110 122 152 182 230 125 229 125 112 140 183 150 172 119 136 153 144 165 218 49.7 69.1 43.5 70 63.2 66.6 54.9 38.6 59.4 57 54.4 57.3 53.9 54.7 54.3 48.3 64.7 56.4 68 48 48.7 43.5 55 49 55.9 56.7 59.7 52.7 51.8 50.1 56.9 44.2 712 850 781 801 326 326 378 338 329 357 333 285 194 182 745 736 545 483 556 727 658 616 521 644 675 450 412 386 463 392 275 205 660 663 278 261 220 102 255 223 217 267 226 289 178 9298 10763 8894 10961 7463 8625 7767 8907 8903 10486 167 235 269 682 648 554 119 258 213 108 5978 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060111) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060122) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060126) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060614) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ060718) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070123) LMSP03010003 C16 Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG070306) LMSP03010003 C16 Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG061213) LMSP03010003 C16 Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070619) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070717) LMSP03010003 C16 Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070815) LMSP03010003 C16 Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB000) LMSP03010003 C16 Sphingomyelin pmol/mg protein Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080212) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080219) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080226) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090127) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090114) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090401) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010003 C16 Sphingomyelin pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ081209) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP03010003 C16 Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP03010003 C16 Sphingomyelin - 122 - 128 125 154 117 189 120 86 116 119 184 155 93.2 114 208 177 201 153 143 144 116 101 173 161 101 111 128 157 143 123 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP03010003 C16 Sphingomyelin - 270 - 159 200 184 155 92.8 180 182 140 129 228 153 117 128 245 271 153 187 188 166 127 150 224 191 156 108 134 212 114 149 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP03010003 C16 Sphingomyelin - 163 - 171 171 245 242 181 168 187 248 191 203 187 225 174 217 190 344 290 204 195 209 290 231 285 302 244 156 289 293 309 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP03010003 C16 Sphingomyelin - 1192 - 1309 1406 1605 1315 1262 1468 1017 1233 1325 1455 1378 1339 1391 1491 1458 1557 1385 1558 1606 1578 1531 1708 1334 1390 1667 1502 1342 1453 1380 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP03010003 C16 Sphingomyelin - 2000 - 1495 1651 1625 1375 1012 1669 1809 1386 1494 1749 1707 1435 1385 1897 1787 1432 1325 1885 1770 1514 1555 1886 1849 1675 1503 1335 1964 1227 1496 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP03010003 C16 Sphingomyelin - 1304 - 1276 1244 1418 1436 1287 1230 1164 1610 1393 1414 1239 1281 1356 1484 1298 1543 1370 1380 1301 1581 1623 1191 1306 1625 1731 833 1411 1355 1595 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMSP03010003 C16 Sphingomyelin pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMSP03010003 C16 Sphingomyelin pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120120) LMSP03010003 C16 Sphingomyelin pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120131) LMSP03010003 C16 Sphingomyelin pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120131) LMSP03010003 C16 Sphingomyelin pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120308) LMSP03010003 C16 Sphingomyelin pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0004) LMSP03010003 C16 Sphingomyelin pmol/mg Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0007) LMSP03010003 C16 Sphingomyelin pmol/ml Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0006) LMSP03010003 C16 Sphingomyelin pmol/ml Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070122) LMSP03010003 C16 Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070124) LMSP03010003 C16 Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB300) LMSP03010003 C16 Sphingomyelin pmol/mg protein