Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: C18:1DH Cer Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 0.710.08 0.1 0.21 0.22 0.18 0.09 0.58 0.32 0.47 0.21 0.27 0.2 0.4 0.12 0.48 0.29 0.07 0.48 0.33 0.05 0.27 0.37 0.22 - 0.04 0.04 - - 0.04 0.06 0.2 0.11 0.09 - - 0.06 0.06 - 0.09 0.2 - - - 0.45 - - 0.1 0.164 0.245 0.173 0.768 0.466 0.247 0.153 0.333 1.03 1.39 1.25 3.47 4.35 2.42 1.38 3.01 0.048 0.104 0.055 - 0.057 0.087 0.265 - 0.082 0.122 0.086 0.384 0.233 0.124 0.077 0.166 0.068 0.092 0.082 0.228 0.286 0.159 0.091 0.198 0.409 0.12 0.106 - 0.393 0.08 0.459 0.089 0.489 0.099 0.133 - 0.335 0.066 0.454 0.111 0.025 0.011 0.01 0.03 0.035 - 0.018 0.026 0.022 0.011 0.009 0.033 0.037 - 0.018 0.024 0.129 0.031 0.1 0.044 0.046 0.058 0.026 0.08 0.068 0.055 4.22 1.63 3.36 1.22 0.934 2.47 2.06 0.719 1.41 1.46 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080212) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080219) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080226) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090127) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090114) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090401) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP02020015 C18:1DH Cer pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ081209) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP02020015 C18:1DH Cer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP02020015 C18:1DH Cer - 0.01 - 0.01 0.02 0.01 0.01 0.03 - 0.02 0.01 0.02 0.01 0.02 0.02 0.01 0.02 0.01 0.02 0.02 0.01 - 0.01 0.01 0.01 0.01 0.01 0.02 0.01 0.02 0.02 0.01 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP02020015 C18:1DH Cer - 0.05 - 0.01 0.03 0.05 0.04 0.06 0.03 0.03 0.03 0.02 0.06 0.03 0.02 0.02 0.04 0.03 0.02 0.05 0.03 0.02 0.03 - 0.03 0.02 0.03 0.02 0.03 0.03 0.03 0.02 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP02020015 C18:1DH Cer - 0.01 - 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.01 0.02 0.02 0.01 0.01 0.01 0.02 0.02 0.02 0.01 0.01 0.02 - 0.02 0.01 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP02020015 C18:1DH Cer - 0.1 - 0.1 0.23 0.1 0.11 0.2 - 0.24 0.11 0.22 0.08 0.18 0.29 0.12 0.14 0.08 0.16 0.18 0.11 - 0.14 0.15 0.1 0.08 0.14 0.3 0.12 0.17 0.2 0.11 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP02020015 C18:1DH Cer - 0.37 - 0.09 0.25 0.44 0.35 0.65 0.28 0.3 0.3 0.23 0.46 0.34 0.24 0.22 0.31 0.2 0.19 0.35 0.3 0.21 0.36 - 0.25 0.19 0.32 0.28 0.3 0.28 0.32 0.2 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP02020015 C18:1DH Cer - 0.08 - 0.07 0.07 0.06 0.06 0.07 0.07 0.06 0.07 0.07 0.07 0.07 0.06 0.08 0.07 0.07 0.09 0.09 0.07 0.07 0.08 0.11 0.1 0.09 0.05 0.07 0.11 - 0.09 0.05 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0002) C18:1DH Cer pmol/mg Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0002) LMSP02020015 C18:1DH Cer pmol/ml