Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: C18:1DH Sphingomyelin Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 7.297.1 9.56 6.28 5.24 5.41 4.85 4.2 8.05 7.74 7.99 7.32 5.82 4.79 4.37 4.71 5.88 4.84 5.77 6.97 5.15 4.35 2.88 3.12 6.56 5.91 5.3 6.39 6.77 6.18 6.31 6.61 6.68 6.88 6.12 6.43 5.64 6.76 5.19 7.31 10.6 11.2 9.08 9.26 6.51 6.6 8.65 5.89 24.2 33.3 19.3 31.4 15.5 15.2 19.3 - 151 189 140 142 145 149 174 0.001 0.003 0.009 0.004 - 0.005 0.009 0.024 - 12.1 16.6 9.67 15.7 7.77 7.6 9.65 - 9.95 12.4 9.19 9.33 9.54 9.76 11.4 0 5.58 6.5 4.77 7.4 6.96 7 6.51 4.12 6.66 5.36 5.97 6.06 5.94 5.75 6.43 5.15 6.77 5.8 6.32 5.7 5.12 5.06 6.69 5.53 5.85 5.83 5.55 6.26 5.44 5.83 6.92 4.99 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080212) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080219) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080226) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090127) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090114) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090401) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010031 C18:1DH Sphingomyelin pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ081209) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP03010031 C18:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP03010031 C18:1DH Sphingomyelin - 8.94 - 9.83 9.29 11.2 7.86 13.4 7.62 5.97 8.08 8.12 13.4 11.4 6.26 8.31 14.8 13.5 14.4 11.1 9.21 10.4 8.15 6.84 12.1 12.7 8.28 8.08 9.32 12.6 10.2 8.15 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP03010031 C18:1DH Sphingomyelin - 18.1 - 11.6 14.2 10.7 9.67 8.21 12.4 13.7 9.25 8.67 15.7 11.3 8.19 9.95 16.3 21.1 10.3 12.6 13.2 12.3 7.5 - 13.9 14.6 10.4 7.65 10.8 14.4 7.21 11.6 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP03010031 C18:1DH Sphingomyelin - 8.82 - 10 10.1 15.5 14.2 11.7 9.88 11.4 16 11.8 11.9 11.6 12.3 10.4 12.9 11 22.1 16.8 10.7 11.1 12.1 17.8 12.1 15.5 17.7 13.7 7.87 13.6 16.7 15.1 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP03010031 C18:1DH Sphingomyelin - 87.5 - 101 105 116 88.3 89.5 93.5 70.6 86.1 90.5 106 101 89.9 102 106 111 111 101 100 116 111 104 120 105 114 121 110 107 103 91.5 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP03010031 C18:1DH Sphingomyelin - 134 - 109 118 94.9 85.8 89.5 115 136 91.9 101 121 126 100 108 127 139 96.3 89.2 132 131 89.5 - 117 141 111 106 108 133 77.4 116 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP03010031 C18:1DH Sphingomyelin - 70.7 - 74.8 73.2 89.3 84.2 82.9 72.5 71.2 104 86.4 82.6 76.4 70 81.5 88.4 75 99.2 79.1 72.3 73.9 91.7 99.5 62.3 70.9 95.3 97.2 42.1 66.1 77.4 78.1 pmol/sample