Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: C18DH Sphingomyelin

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
2.782.682.757.46.345.96.575.354.223.321.114.61713.5108.645.11109.2313.56.297.426.333.061.880.723 0.543 8.89 5.08 4.52 5.76 2.65 1.97 1.53 0.88 0.443 37 2.68 2.23 3.7 1.84 1.44 2.36 1.96 1.43 3.37 4.27 3.46 3.1 2.32 2.04 1.64 1.52 2.23 2.25 2.31 2.52 2.06 1.7 1.08 0.72 1.37 1.39 1.5 1.23 0.84 0.85 1.13 0.69 3.41 4.74 2.69 4.32 2.12 2.15 2.82 2.07 21.4 27 19.4 19.6 19.8 21 25.4 18.7 0.993 0.628 0.773 0.641 0.606 0.563 0.644 0.709 1.71 2.37 1.34 2.16 1.06 1.08 1.41 1.03 1.4 1.77 1.28 1.29 1.3 1.38 1.67 1.23 0.827 1.09 0.746 1.27 0.991 1.26 1.04 0.638 0.988 0.9 0.934 1.04 0.846 1.04 1.03 0.797 0.838 0.772 0.793 0.665 0.657 0.566 0.836 0.686 0.724 0.777 0.696 0.73 0.699 0.653 0.864 0.618 6.92 8.02 7.89 8.09 3.84 5.95 6.13 5.96 5.2 5.32 5.88 4.78 4.56 6.18 12.3 9.72 6.74 6.44 9.03 9.8 7.41 8 6.22 14.6 12.5 8.27 5.86 6.65 9.42 7.26 4.6 3.41 15.4 12 5.58 22.7 16.2 14.3 29.2 20.2 13.8 10.9 16.9 17.4 9.58 1800 983 853 2328 1406 2997 578 2095 479 1036 37.3
Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060111)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060122)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060126)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060614)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ060718)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070123)		LMSP03010020 C18DH Sphingomyelin pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG070312)		LMSP03010020 C18DH Sphingomyelin pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG061213)		LMSP03010020 C18DH Sphingomyelin pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070619)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070515)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070515)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD070814)		LMSP03010020 C18DH Sphingomyelin pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCISUB000)		LMSP03010020 C18DH Sphingomyelin pmol/mg protein
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080212)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080219)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080226)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090401)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP03010020 C18DH Sphingomyelin pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ081209)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMSP03010020 C18DH Sphingomyelin pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMSP03010020 C18DH Sphingomyelin -
1.52
-
1.36		 1.67
1.87
1.16
1.84		 1.42
0.9
1.2
1.15		 2.24
2.05
0.97
1.19		 2.73
2.39
2.16
1.62		 1.7
1.93
1.34
1.15		 2.25
2.03
1.25
1.19		 1.51
1.92
1.48
1.25		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		LMSP03010020 C18DH Sphingomyelin -
1.89
-
0.92		 1.95
1.49
1.22
0.86		 2.18
2
1.27
1.18		 2.57
1.79
1.24
1.48		 3.07
3.78
1.7
2.05		 2.34
2.05
1.32
-		 2.55
2.55
1.78
1.26		 1.65
2.32
0.96
1.84		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		LMSP03010020 C18DH Sphingomyelin -
1.44
-
1.67		 1.7
2.71
2.51
1.94		 1.66
1.91
2.77
2.1		 2.31
2.08
2.15
1.97		 2.13
1.96
3.79
3.08		 1.85
1.89
2.34
3.21		 1.99
2.78
3.3
2.44		 1.37
2.42
2.98
2.8		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMSP03010020 C18DH Sphingomyelin -
14.9
-
13.9		 18.9
19.4
13
12.3		 17.4
10.6
12.8
12.8		 17.7
18.2
13.9
14.6		 19.6
19.7
16.8
14.7		 18.5
21.5
18.2
17.4		 22.2
16.8
17.2
17.9		 17.8
16.4
15
14		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		LMSP03010020 C18DH Sphingomyelin -
14
-
8.65		 16.1
13.2
10.8
9.38		 20.2
19.9
12.6
13.7		 19.8
20
15.2
16		 23.8
25
15.9
14.5		 23.5
21.9
15.8
-		 21.5
24.7
19.1
17.5		 16.4
21.5
10.3
18.5		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		LMSP03010020 C18DH Sphingomyelin -
11.5
-
12.5		 12.4
15.7
14.9
13.8		 12.2
11.9
18
15.3		 16.1
13.8
12.3
15.4		 14.6
13.4
17
14.5		 12.5
12.6
17.7
18		 10.3
12.7
17.8
17.3		 7.32
11.8
13.8
14.5		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		LMSP03010020 C18DH Sphingomyelin pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		LMSP03010020 C18DH Sphingomyelin pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120120)		LMSP03010020 C18DH Sphingomyelin pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120131)		LMSP03010020 C18DH Sphingomyelin pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120131)		LMSP03010020 C18DH Sphingomyelin pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120308)		LMSP03010020 C18DH Sphingomyelin pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0003)		LMSP03010020 C18DH Sphingomyelin pmol/mg
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0010)		LMSP03010020 C18DH Sphingomyelin pmol/ml
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070122)		LMSP03010020 C18DH Sphingomyelin pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070124)		LMSP03010020 C18DH Sphingomyelin pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCISUB300)		LMSP03010020 C18DH Sphingomyelin pmol/mg protein