Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: C20DH GlcCer

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
0.0880.007 0.051 0.022 0.166 0.289 0.206 0.069 0.163 0.044 0.083 0.53 0.382 0.297 0.14 - - - - 0.801 0.944 0.655 - 0.725 0.16 0.1 - - 0.103 0.037 0.09 0.033 0.03 0.013 0.01 0.01 - 0.15 0.18 - - 0.46 - - - 1.44 1.47 - 1.54 2.42 1.28 0.19 - 2.15 1.07 0.43 1.02 0.99 1.08 0.31 0.26 - 0.01 - - - 0.02 - - 1.05 0.278 - - - - 0.357 - 6.6 1.58 - - - - 3.22 - - 0.235 0.003 0.004 - 0.086 0.057 - 0.527 0.139 - - - - 0.178 - 0.434 0.104 - - - - 0.211 - - - - - - - - - - - - - - - - - 0.153 0.03 0.15 0.018 0.137 0.235 0.048 0.083 0.132 0.03 0.132 0.02 0.146 0.271 0.05 0.075 0.123 0.085
Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCISUB000)		LMSP0501AA20 C20DH GlcCer pmol/mg protein
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060111)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060122)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060126)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060614)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ060718)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070123)		LMSP0501AA20 C20DH GlcCer pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG070319)		LMSP0501AA20 C20DH GlcCer pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG061213)		LMSP0501AA20 C20DH GlcCer pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070619)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070515)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070515)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD070815)		LMSP0501AA20 C20DH GlcCer pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080212)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080219)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080226)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090401)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP0501AA20 C20DH GlcCer pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ081209)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMSP0501AA20 C20DH GlcCer pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMSP0501AA20 C20DH GlcCer -
0.04
-
0.02		 -
0.04
-
0.09		 0.06
0.03
0.02
0.08		 0.05
0.02
0.04
0.02		 0.07
0.04
0.03
0.06		 0.02
0.13
0.03
0.03		 0.06
-
0.03
0.05		 0.03
-
0.05
0.05		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		LMSP0501AA20 C20DH GlcCer -
0.1
-
0.09		 0.08
0.04
0.09
0.05		 0.06
0.07
0.05
0.06		 0.11
0.04
0.04
0.04		 0.1
0.11
0.08
0.1		 0.04
0.04
0.03
-		 0.06
0.04
0.05
0.04		 0.06
0.08
0.06
0.06		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		LMSP0501AA20 C20DH GlcCer -
0.01
-
-		 0.01
0.02
0.03
0.02		 0.03
0.01
0.05
0.02		 0.02
0.03
0.05
0.05		 0.02
0.02
0.02
0.03		 -
0.02
0.03
0.03		 0.07
0.02
0.08
0.03		 0.02
0.05
-
0.04		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMSP0501AA20 C20DH GlcCer -
0.39
-
0.2		 -
0.42
-
0.6		 0.74
0.35
0.21
0.89		 0.4
0.18
0.57
0.24		 0.5
0.33
0.23
0.54		 0.22
1.45
0.41
0.45		 0.59
-
0.41
0.75		 0.35
-
0.51
0.56		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		LMSP0501AA20 C20DH GlcCer -
0.74
-
0.85		 0.66
0.35
0.8
0.55		 0.56
0.7
0.5
0.7		 0.85
0.45
0.49
0.43		 0.78
0.73
0.75
0.71		 0.4
0.43
0.36
-		 0.51
0.39
0.54
0.55		 0.6
0.74
0.64
0.6		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		LMSP0501AA20 C20DH GlcCer -
0.08
-
-		 0.07
0.12
0.18
0.14		 0.22
0.06
0.33
0.15		 0.14
0.2
0.28
0.39		 0.14
0.14
0.09
0.14		 -
0.13
0.23
0.17		 0.36
0.09
0.43
0.21		 0.11
0.24
-
0.21		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		LMSP05010020 C20DH GlcCer pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070122)		LMSP0501AA20 C20DH GlcCer pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070124)		LMSP0501AA20 C20DH GlcCer pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCISUB300)		LMSP0501AA20 C20DH GlcCer pmol/mg protein