Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: C24:1DH GlcCer Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 0.4760.273 0.588 0.192 0.13 0.217 0.447 0.443 0.488 0.248 0.377 2.12 0.864 0.847 1.19 - - - 0.54 1.93 0.476 0.294 1.01 2.58 0.654 0.36 0.23 0.22 0.963 0.44 0.413 0.35 0.303 0.213 0.01 0.01 0.01 0.07 0.35 - 0.18 0.31 0.26 0.16 0.14 - - - 1.46 - - 0.03 0.56 - - - - - - - - 2.57 1.54 1.74 1.8 1.77 0.78 1.15 0.49 0.105 1.21 0.72 2.51 0.732 0.379 0.758 - 0.657 6.9 5.21 11.4 6.84 3.71 6.84 - 1.27 - 0.401 0.399 0.476 0.694 0.251 0.084 0.053 0.606 0.36 1.26 0.366 0.189 0.379 - 0.043 0.453 0.342 0.747 0.449 0.243 0.449 - 0.067 - 0.057 - 0.27 - - 0.118 0.081 - 0.071 - 0.23 - - 0.147 0.023 0.223 0.356 0.221 0.409 0.143 0.493 - 0.02 0.224 0.313 0.242 0.435 0.165 0.51 - 1.16 0.38 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB000) LMSP0501AA22 C24:1DH GlcCer pmol/mg protein Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060111) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060122) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060126) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060614) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ060718) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070123) LMSP0501AA22 C24:1DH GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG070306) LMSP0501AA22 C24:1DH GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG061213) LMSP0501AA22 C24:1DH GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070619) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070816) LMSP0501AA22 C24:1DH GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080212) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080219) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080226) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090401) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA22 C24:1DH GlcCer pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ081209) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP0501AA22 C24:1DH GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP0501AA22 C24:1DH GlcCer - 0.24 - - - 0.2 - - - 0.01 0.02 - 0.15 - 0.03 0.01 0.01 0.13 - - - - 0.11 - 0.16 0.01 - - 0.03 - - - pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP0501AA22 C24:1DH GlcCer - 0.39 - 0.16 0.59 0.32 0.45 0.51 0.36 0.48 0.61 0.47 0.58 0.48 0.51 0.47 0.97 1.27 0.79 0.98 0.44 0.45 0.5 - 0.57 0.38 0.38 0.37 0.35 0.62 0.46 0.4 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP0501AA22 C24:1DH GlcCer - 0.22 - 0.2 0.28 0.83 0.45 0.26 0.32 0.36 0.4 0.5 0.24 0.33 0.39 0.37 0.39 0.32 0.6 0.4 0.13 0.4 0.62 0.51 0.62 0.75 0.51 0.37 0.53 0.79 1 0.74 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP0501AA22 C24:1DH GlcCer - 2.35 - - - 2.08 - - - 0.12 0.21 - 1.19 - 0.43 0.12 0.07 1.07 - - - - 1.5 - 1.58 0.08 - - 0.35 - - - pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP0501AA22 C24:1DH GlcCer - 2.89 - 1.5 4.88 2.83 3.99 5.56 3.33 4.78 6.06 5.45 4.46 5.36 6.23 5.09 7.52 8.39 7.39 6.95 4.42 4.81 5.97 - 4.81 3.67 4.08 5.13 3.49 5.75 4.94 4.02 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP0501AA22 C24:1DH GlcCer - 1.76 - 1.49 2.03 4.8 2.67 1.85 2.35 2.24 2.6 3.65 1.67 2.18 2.22 2.89 2.67 2.18 2.69 1.89 0.88 2.67 4.69 2.85 3.2 3.44 2.74 2.62 2.83 3.85 4.63 3.83 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMSP05010022 C24:1DH GlcCer pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070122) LMSP0501AA22 C24:1DH GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070124) LMSP0501AA22 C24:1DH GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB300) LMSP0501AA22 C24:1DH GlcCer pmol/mg protein