Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: C24:1DH Sphingomyelin Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 6063.370.441.536.834.943.543.146.657.164.347.961.656.159.467.670.323.722.229.818.526.830.531.316.113.7 16.1 99.9 109 131 79.3 67.3 60.6 12.1 6.94 3.82 263 29.4 21.4 39.5 21 21.5 37 32.4 31.3 27.6 37.9 38.9 36.6 27.2 28.2 30.6 36.8 22.6 20.9 26.8 29.9 26.2 28.8 21.5 17.7 55.4 44.5 45.4 41.7 30.3 31.9 41.9 30.2 68.8 86.2 54.6 85.9 40.1 42.5 49.2 33.5 430 491 395 389 375 416 444 304 25.4 17.3 20.3 14 17.8 14.8 19.8 24.5 34.4 43.1 27.3 42.9 20.1 21.3 24.6 16.8 28.3 32.2 25.9 25.5 24.6 27.3 29.2 19.9 12.2 14.8 11.1 17 14.4 14.9 13.5 7.53 14.6 12.2 13.8 14 12.3 12.2 13.3 9.41 14.8 12.8 15.1 11.7 12.1 9.54 15.6 11.1 12.8 12.8 13.3 12.9 12.8 11 16.1 9.98 110 134 147 168 133 99.7 105 119 93.2 79.2 110 92.5 124 203 140 135 97.7 88.5 131 184 152 190 164 158 183 102 87.9 99.5 128 121 92.4 92.1 144 123 149 275 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060111) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060122) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060126) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060614) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ060718) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070123) LMSP03010023 C24:1DH Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG070306) LMSP03010023 C24:1DH Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG061213) LMSP03010023 C24:1DH Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070619) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070815) LMSP03010023 C24:1DH Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB000) LMSP03010023 C24:1DH Sphingomyelin pmol/mg protein Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080212) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080219) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080226) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090401) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010023 C24:1DH Sphingomyelin pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ081209) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP03010023 C24:1DH Sphingomyelin pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP03010023 C24:1DH Sphingomyelin - 14.7 - 18.7 18.5 22.3 13.3 21.3 13.7 10.8 14.9 14.7 24.9 22.4 12 15 29.4 25.5 25.3 19.8 17.6 18.7 15.9 12.9 25.9 22.6 14.2 14.7 20.1 21.1 21 16.5 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP03010023 C24:1DH Sphingomyelin - 27.8 - 15.8 23.5 16.7 15.5 13 26.5 25.2 17.9 15.1 34 22.7 16.8 19.7 37.2 46 21.8 29.2 26.9 24.9 15.2 - 34.7 31 23.7 15.3 26.4 28.7 15.9 23.1 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP03010023 C24:1DH Sphingomyelin - 19.3 - 20.3 22 39.8 30.7 22.4 20.9 24.8 37.5 25.2 29.2 29.1 24.4 24.4 34.3 29.3 46.2 36.7 25.5 27.7 36.1 45.1 37.8 51.7 47.6 36.9 28.7 42.7 48.2 45.3 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP03010023 C24:1DH Sphingomyelin - 144 - 191 208 231 150 142 168 127 159 164 197 199 172 183 211 210 196 180 191 208 216 196 256 187 195 220 237 181 213 185 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP03010023 C24:1DH Sphingomyelin - 206 - 149 194 147 137 142 246 251 178 175 261 253 206 213 289 304 204 207 271 266 181 - 292 300 255 213 263 266 170 232 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP03010023 C24:1DH Sphingomyelin - 155 - 151 160 230 182 159 154 154 244 184 203 193 139 191 235 200 207 173 172 185 273 252 195 237 256 261 154 208 223 234 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMSP03010023 C24:1DH Sphingomyelin pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMSP03010023 C24:1DH Sphingomyelin pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120120) LMSP03010023 C24:1DH Sphingomyelin pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120131) LMSP03010023 C24:1DH Sphingomyelin pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120131) LMSP03010023 C24:1DH Sphingomyelin pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120308) LMSP03010023 C24:1DH Sphingomyelin pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070122) LMSP03010023 C24:1DH Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070124) LMSP03010023 C24:1DH Sphingomyelin pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB300) LMSP03010023 C24:1DH Sphingomyelin pmol/mg protein