Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: C24 GlcCer Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 5.873.280.645.585.045.375.736.516.884.4418.214.115.314.417.519.112.62.74.645.515.126.927.715.221.941.92 1.33 7.84 5.84 3.03 6.31 3.51 1.19 0.147 0.093 0.053 86.1 2.43 1.85 3.64 2.08 2.92 1.8 1.11 0.5 1.09 4.44 3.31 2.72 3.1 3.32 2.21 1.19 2.39 1.91 2.24 1.92 1.99 1.36 1.17 0.49 2.26 2.01 2.07 2.54 3.78 2.02 2.87 3.23 3.92 5.22 3.89 4.33 3.54 3.39 3.38 4.85 5.9 5.01 5.59 5.27 3.54 3.18 4.75 3.24 15.3 22.4 10.5 17.9 7.95 8.24 10.1 4.16 95.5 127 76.2 81.3 74.3 80.6 91.4 37.6 1.62 1.28 2.32 1.4 2.43 1.98 1.63 2.25 7.63 11.2 5.27 8.97 3.98 4.12 5.07 2.08 6.28 8.37 5 5.34 4.88 5.29 6.01 2.47 1.8 1.37 0.948 1.85 2.01 1.57 1.63 1.17 2.15 1.13 1.19 1.52 1.72 1.29 1.61 1.46 2.86 2.3 2.21 1.73 2.41 1.85 2.48 2.14 2.47 2.32 1.94 1.89 2.57 2.14 2.56 1.93 12 84.4 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060111) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060122) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060126) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCI060614) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ060718) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070123) LMSP0501AA09 C24 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG070312) LMSP0501AA09 C24 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG061213) LMSP0501AA09 C24 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070619) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070724) LMSP0501AA09 C24 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070814) LMSP0501AA09 C24 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB000) LMSP0501AA09 C24 GlcCer pmol/mg protein Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080212) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080219) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080226) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090127) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090114) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090401) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA09 C24 GlcCer pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ081209) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMSP0501AA09 C24 GlcCer pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP0501AA09 C24 GlcCer - 0.75 - 1.73 1.5 0.9 1.97 1.06 0.51 0.24 0.67 0.74 1.05 0.77 0.71 0.66 0.8 1.28 1.23 1.34 1.3 0.81 0.72 0.66 1.28 1.29 0.55 0.95 0.29 0.98 2.3 0.96 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP0501AA09 C24 GlcCer - 11.3 - 5.42 5.27 3.31 4.65 3.73 4.43 4.22 4.23 4.13 5.91 4.36 5.31 3.46 9.06 7.54 8.01 8.23 3.2 3.46 3.95 7.51 4.82 3.3 4.05 3.56 5.32 5.61 6.87 3.93 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP0501AA09 C24 GlcCer - 4.51 - 5.23 4.7 9.84 7.85 5.49 4.99 5.67 10.1 6.87 6.35 7.24 7.21 6.13 7.34 6.55 12.6 9.4 7.49 6.94 10.7 12.1 12 15.1 15.1 10.8 10.9 14.5 27.1 17.7 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMSP0501AA09 C24 GlcCer - 7.34 - 17.7 16.9 9.35 22.1 7.07 6.26 2.84 7.14 8.25 8.3 6.84 10.2 8.08 5.74 10.6 9.54 12.1 14.2 9.04 9.79 9.99 12.7 10.7 7.57 14.3 3.42 8.38 23.4 10.8 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMSP0501AA09 C24 GlcCer - 83.9 - 51 43.6 29.3 41.3 40.7 41 42 42 47.9 45.4 48.7 64.9 37.4 70.3 49.8 74.9 58.3 32.2 37 47.2 78 40.7 31.9 43.4 49.4 53 52 73.7 39.5 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMSP0501AA09 C24 GlcCer - 36.1 - 39 34.1 56.9 46.5 39 36.6 35.3 65.6 50.2 44.2 47.9 41.1 47.9 50.3 44.6 56.5 44.4 50.6 46.4 80.7 67.4 62 68.9 81.2 76.8 58.1 70.6 126 91.4 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMSP05010009 C24 GlcCer pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070122) LMSP0501AA09 C24 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070124) LMSP0501AA09 C24 GlcCer pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB300) LMSP0501AA09 C24 GlcCer pmol/mg protein