Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

Introduction | Browse | Download

---

Mouse-over the above links for more information

Details: Desmosterol Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 1.190.24 0.203 0.25 0.203 0.23 0.237 0.253 13.1 12.9 17.8 14 11.8 9.49 11.2 30 20.3 19.8 19.3 22 22 24.5 29.5 38.6 361 379 394 310 341 305 356 351 270 288 291 404 353 289 281 388 99.6 123 126 116 137 177 208 238 32.2 42 36.1 39.3 31.3 34.1 37.7 39.2 35.5 36.8 44.4 40.5 38.3 38 44.3 52.4 22.8 26.1 30.5 34 41.2 39.7 36.7 50.1 27.4 25.8 23.6 27.1 30.3 25.1 32.7 46.8 24.9 24.2 17.1 17.2 23.2 17.9 28.1 38.1 11.8 12.1 13.1 14.7 14.5 15.6 21.6 32.6 88.6 109 72.7 108 56.5 63.8 84.1 78.7 72.9 81.1 69.1 64.2 69.3 82 99.7 93.6 37 51 26.6 49.9 46.2 63.4 55.2 30.5 44.2 42.1 33.2 40.9 39.4 52.1 54.6 38.1 42.9 36.5 42.2 30.4 31.4 31.1 38.8 35.4 37.1 36.7 37 33.4 33.4 35.8 40.1 31.9 27.2 29.5 27.2 31 43.9 73.9 1.49 1.38 2.59 8.04 8.44 2.99 9.91 8.76 11.4 12.2 12.1 15.2 13.8 25.3 15.3 19.2 18.5 18.1 19.3 21.5 27.3 38 9.15 8.42 12.2 9.65 14.2 12 15.9 36.9 21.3 20.5 22.3 16.6 23.7 18.2 19.2 26 8.12 8.59 15.7 185 288 518 473 1207 391 183 194 181 201 272 285 280 302 87.2 64 82.3 77.2 75.7 79.1 81.9 71.7 56.5 101 124 116 96.7 88.6 95.5 116 115 108 146 82.7 114 81.1 66.9 62 79.8 82.1 74 87.6 38.1 37.3 40.2 8.94 7.12 5.02 5.41 4.01 3.06 1.63 15.7 1.95 6.64 2.97 8.27 13.1 2.89 1.29 3.43 5.24 3.98 2249 298 2005 2308 1942 853 3693 1031 1392 1815 765 1463 699 2413 2458 2169 2493 857 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070605) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070612) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070820) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ071011) LMST01010016 Desmosterol pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070726) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070808) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070823) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080212) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080219) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ080226) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD080211) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD080311) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD080408) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090512) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090716) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD090805) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010016 Desmosterol - 61.1 - 54.3 63 60.6 60.7 47 56.2 37.9 54.2 46.6 68.4 46.6 46.6 50.5 63.1 77.6 62.6 81.1 45.1 40.9 47.7 57.6 - 37.7 48.3 47.3 38.9 26.4 44.6 61.1 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010016 Desmosterol - 46.5 - 68 53.7 78.8 61.5 68.4 50.7 56.1 79.2 63.4 49.7 51.9 77.2 59.3 49.4 46.6 106 102 45.1 45.2 59.9 89.3 55.4 54.6 85.1 70.2 45.6 38.5 93.9 98.6 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010016 Desmosterol - 597 - 555 711 629 682 314 689 448 578 520 541 414 670 618 453 639 486 735 490 456 649 872 - 313 664 710 459 226 453 686 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010016 Desmosterol - 455 - 695 607 819 691 456 622 663 844 706 393 461 1109 726 355 384 818 924 491 504 814 1351 548 453 1171 1053 537 329 954 1107 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070515) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070619) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ070724) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ050725) LMST01010016 Desmosterol ug/extract Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ060926) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ061017) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ061024) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ061024) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJ061010) LMST01010016 Desmosterol pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ070724) LMST01010016 Desmosterol pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCJSUB003) LMST01010016 Desmosterol pmol/mg protein Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCISUB300) Desmosterol pmol/mg protein Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ110501) LMST01010016 Desmosterol pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMST01010016 Desmosterol umol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMST01010016 Desmosterol umol/ml of media Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) Desmosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120120) LMST01010016 Desmosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120131) LMST01010016 Desmosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120212) LMST01010016 Desmosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120308) LMST01010016 Desmosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0002) LMST01010016 Desmosterol pmol/mg Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0007) LMST01010016 Desmosterol pmol/ml