Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: Lanosterol

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
1.570.827 0.683 0.78 0.71 0.743 0.683 0.767 2.55 3.04 2.82 3.77 4.78 3.28 3.26 3.99 1.84 0.813 0.667 0.703 0.617 0.63 0.635 0.623 1.79 2.4 2.44 2.16 1.92 1.78 2 2.11 2.18 2.06 2.71 2.45 2.58 2.91 3.74 1.83 1.32 1.68 1.76 1.1 1.62 1.01 1.99 1.44 0.4 0.33 0.36 0.31 0.3 0.57 0.25 0.15 0.42 0.28 0.41 0.4 0.43 0.6 0.44 0.27 0.3 0.2 0.3 0.28 0.31 0.47 0.3 0.12 0.52 0.58 0.37 0.4 0.45 0.41 0.74 0.45 0.88 2.4 1.75 1.29 0.97 0.57 13.2 8.17 2.15 2.88 2.17 1.75 2.18 2.21 2.66 2.42 - 3.62 2.17 5.22 2.13 2.29 2.25 2.62 - 2.7 2.06 3.11 2.62 2.94 2.67 3.11 2.21 2.02 1.3 1.5 1.75 2.05 1.59 0.709 2.63 1.66 1.62 1.23 1.49 1.69 1.57 0.887 1.08 0.998 0.898 0.617 0.849 0.832 1.03 0.584 0.93 1 0.789 0.677 0.903 0.959 1.07 0.527 1.74 2.2 1.45 3.62 5.33 2.22 0.537 1.06 1.71 0.666 0.204 0.047 1.78 1.48 1.7 1.44 1.66 1.88 1.48 2.09 3.71 3.98 3.58 4.04 3.98 4.27 5.23 5.72 6.07 4.74 4.43 3.3 3.26 3.78 6.74 12.4 6.79 6.24 6.6 5.42 5.95 4.89 3.82 5.42 1.11 4.47 7.31 17.2 12.1 31.8 29.6 24.8 14.7 3.11 3.09 4.82 4.54 4.43 4.14 4.13 5.34 6.18 7.73 8.12 5.96 6.57 6.89 7.31 5.27 4.25 7.49 6.5 5.34 4.15 5.66 6.48 6.5 6.07 5.25 6.18 8.23 5.97 5.15 4.6 5.68 7.53 5.26 3.28 6.52 5.14 5.26 1.48 5.14 0.01 0.004 1.79 5.16 0.786 0.739 2.46 0.002 0.006 0.027 0.002 4.84 0.004 0.01 0.016 0.289 1.28 390 0.513 102 293 290 0.653 0.697 0.317 1.06 0.307 158 1.04 394 0.331 176 91.2 0.203
Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070605)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070612)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070820)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ071011)		LMST01010017 Lanosterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070726)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070808)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070823)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080212)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080219)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080226)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD080211)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD080311)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD080408)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010017 Lanosterol -
0.09
-
0.43		 0.05
0.04
0.67
0.49		 0.09
0.04
0.36
0.4		 0.11
0.05
0.14
0.39		 0.06
0.06
0.26
0.66		 0.06
0.01
0.17
0.41		 -
0.04
0.16
0.37		 0.02
0.02
0.09
0.41		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010017 Lanosterol -
1.56
-
7.45		 1.6
1.85
8.67
7.12		 1.49
1.76
6.46
6.9		 1.35
1.67
4.94
6.7		 1.61
1.4
4.33
10.8		 1.65
1.39
2.37
9.81		 1.9
1.67
2.8
6.06		 2.3
0.91
1.83
5.8		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010017 Lanosterol -
0.88
-
4.4		 0.56
0.42
7.53
3.27		 1.1
0.47
3.84
4.46		 0.87
0.44
2.01
4.77		 0.43
0.49
2.02
5.98		 0.65
0.11
2.31
6.21		 -
0.33
2.2
5.55		 0.24
0.17
0.91
4.6		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01010017 Lanosterol -
15.3
-
76.2		 18.1
19.2
97.4
47.5		 18.3
20.8
68.8
76.9		 10.7
14.8
71
82		 11.6
11.5
33.6
97.6		 18
15.5
32.2
148		 18.8
13.9
38.5
90.9		 27.1
7.78
18.6
65.1		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070515)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070619)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070724)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ050725)		LMST01010017 Lanosterol ug/extract
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ060926)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ061017)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ061024)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ061024)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ061010)		LMST01010017 Lanosterol pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070717)		LMST01010017 Lanosterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070927)		LMST01010017 Lanosterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070927)		LMST01010017 Lanosterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ070927)		LMST01010017 Lanosterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJSUB001)		LMST01010017 Lanosterol pmol/mg protein
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCISUB300)		 Lanosterol pmol/mg protein
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(MSJ110501)		LMST01010017 Lanosterol pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		LMST01010017 Lanosterol umol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		LMST01010017 Lanosterol umol/ml of media
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120120)		LMST01010017 Lanosterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120131)		LMST01010017 Lanosterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120212)		LMST01010017 Lanosterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120308)		LMST01010017 Lanosterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0015)		LMST01010017 Lanosterol pmol/mg
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0003)		LMST01010017 Lanosterol pmol/ml