Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: Palmitic acid Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 251138 85.3 238 162 99.1 324 252 114 132 114 108 60.2 52.2 41.6 112 55 102 98.4 78.9 110 86.8 89.2 142 129 117 107 144 121 113 88.4 87.4 87.3 80.8 109 89.8 95.5 79.2 60 75.2 68 58 75.4 60.4 59 68.3 71.8 57.2 59.6 65 44 55.3 35.6 50.7 50.3 73.2 69.6 45.4 54 53.1 50.7 61.1 75.5 188 156 146 137 134 116 154 136 155 117 139 81.4 165 150 182 162 74.6 69.5 64.3 86.3 102 91.7 82.6 55.5 89.1 57.3 80.5 70.7 86.6 75.3 81.6 69.3 84.5 85.8 78.4 64.8 68.7 62.6 79.9 75.2 73 86.4 68.8 71.2 73.1 72.1 82.6 67.7 887 928 861 617 815 674 555 462 140 147 86.8 238 212 512 268 227 170 141 94.2 225 318 231 221 182 107 105 88.6 119 47.6 192 49.1 33.3 40.4 56.1 56.1 1138 1308 1122 1032 1213 1616 1115 706 710 1005 1083 1300 644 863 1158 989 965 564 335 1168 816 505 894 720 721 323 137 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070804) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070612) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070804) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070814) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070817) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080227) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080520) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080520) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080702) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080702) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080702) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090924) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090924) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMFA01010001 Palmitic acid - 48.1 - 30.6 64.6 71.4 34.1 56.5 42.7 38.7 39 40.7 37.4 48.8 11.7 20.2 53.5 36.6 33.6 35.1 38.7 33.1 39.7 18.4 49.2 52.8 40.6 32.3 42 48.5 32.8 34.4 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMFA01010001 Palmitic acid - 129 - 61.4 104 152 57.9 45.5 131 93.2 83.4 57.5 80.7 55.4 66.1 73.5 85.8 97.5 64.8 80 80.7 86.2 72.9 119 124 87.2 106 79.7 136 109 102 100 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMFA01010001 Palmitic acid - 124 - 119 196 222 173 90.5 171 145 130 159 171 136 168 124 228 200 210 154 191 161 237 300 290 283 297 127 405 295 311 254 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMFA01010001 Palmitic acid - 471 - 313 729 742 383 377 523 458 416 453 296 434 168 247 384 302 260 318 421 369 539 279 486 438 558 484 495 415 333 387 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMFA01010001 Palmitic acid - 958 - 577 863 1344 514 497 1216 927 828 667 620 619 808 795 665 644 606 567 811 921 870 1233 1046 843 1132 1104 1358 1007 1094 1005 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMFA01010001 Palmitic acid - 990 - 885 1427 1285 1025 644 1252 899 848 1165 1191 897 958 970 1560 1364 943 729 1287 1075 1788 1678 1499 1298 1596 896 2167 1442 1441 1313 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120308) LMFA01010001 Palmitic acid pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070831) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070927) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070830) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070924) LMFA01010001 Palmitic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070919) LMFA01010001 Palmitic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070814) LMFA01010001 Palmitic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070316) LMFA01010001 Palmitic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070316) LMFA01010001 Palmitic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070316) LMFA01010001 Palmitic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070717) LMFA01010001 Palmitic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070814) LMFA01010001 Palmitic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120120) LMFA01010001 Palmitic acid pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120131) LMFA01010001 Palmitic acid pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120212) LMFA01010001 Palmitic acid pmol/10E6 cells