Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: Sitosterol

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
9.4111.7 11.8 11.7 12.5 18.2 15.6 14.1 8.02 7.86 10.5 5.51 7.67 6.73 4.5 3.78 3.2 8.61 7.54 5.89 4.62 5.47 6.29 5.97 4.35 3.87 8.99 8.47 6.43 5.92 4.87 6.56 5.6 3.55 2.16 3.71 4.52 2.4 8.78 22.7 15.1 15.5 17.4 6028 17.2 17.2 17 10.4 21.9 20.2 15.8 10.3 13.9 28.5 20.1 15.3 3975 11187 3860 3646 2223 8137 2514 2165 12584 7808 6968 5772 5750 4595 5014 3158 3867 18619
Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01040129 Sitosterol -
1.37
-
1.01		 1.2
1.16
0.98
0.78		 0.91
0.87
0.93
0.69		 1.23
0.87
0.79
0.77		 1.64
1.36
0.84
1.43		 0.91
0.9
0.65
0.94		 -
0.85
0.77
0.75		 0.92
0.89
0.67
0.87		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01040129 Sitosterol -
1.17
-
1.13		 1.31
1.85
1.65
1.23		 1.45
1.58
1.47
1.39		 1.07
1.17
1.67
1.29		 1.23
1.24
1.81
1.51		 1.41
1.44
0.95
1.32		 1.71
1.64
2.06
1.17		 1.64
1.56
2.01
1.96		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01040129 Sitosterol -
13.4
-
10.3		 13.6
12.1
11
5.2		 11.2
10.3
9.91
7.69		 9.73
7.73
11.4
9.43		 11.8
11.2
6.51
13		 9.9
10
8.84
14.2		 -
7.05
10.6
11.3		 10.8
7.61
6.8
9.76		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMST01040129 Sitosterol -
11.5
-
11.6		 14.8
19.2
18.5
8.2		 17.8
18.7
15.7
15.5		 8.46
10.4
24
15.8		 8.83
10.2
14
13.7		 15.3
16.1
12.9
20		 16.9
13.6
28.3
17.6		 19.3
13.3
20.4
22		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		LMST01040129 Sitosterol umol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		LMST01040129 Sitosterol umol/ml of media
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120120)		LMST01040129 Sitosterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120131)		LMST01040129 Sitosterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120212)		LMST01040129 Sitosterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120308)		LMST01040129 Sitosterol pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0006)		LMST01040129 Sitosterol pmol/mg
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0011)		LMST01040129 Sitosterol pmol/ml