Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: Sphingosine-P

Legend:graph_icon: View detailed data for replicatesgraph_icon: View time course graphs
Ctrl:Untreated BMDM cells (control)
25-OHC: Cells treated with 25-OH-cholesterol at time 0
*: BMDM cells in lipid-depleted media containing compactin
25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0
1.773.450.6023.892.5 1.62 8.19 8.98 8.29 1.72 1.11 1.4 0.335 0.157 0.127 3.19 3.1 4.89 2.46 2.83 5 1.57 4.28 3.05 2.12 2.78 2.23 1.99 1.07 0.92 1.05 1.8 1.7 1.72 1.84 1.63 0.87 0.92 0.99 0.016 0.022 0.019 0.011 0.012 0.018 0.026 0.018 0.021 0.02 0.017 0.036 0.052 0.051 0.012 0.015 0.016 0.02 0.027 0.034 0.038 0.06 0.05 0.06 0.07 0.03 0.04 0.03 0.04 0.08 0.07 - 0.08 0.02 0.07 0.09 0.06 - - - 0.01 - - - - - 0.024 0.032 - - - - - - 0.137 0.231 - - - - - 0.04 0.045 0.055 0.061 0.051 0.058 0.061 0.07 0.009 0.016 0.006 0.02 0.006 0.005 0.012 0.018 0.008 0.012 0.006 0.012 0.008 0.007 0.014 0.021 0.006 0.01 0.005 0.011 0.013 0.008 0.011 0.009 0.007 0.008 0.007 0.009 0.011 0.006 0.01 0.012 0.01 0.006 0.007 0.005 0.005 0.006 0.006 0.012 0.009 0.006 0.006 0.006 0.006 0.007 0.006 0.011 0.197 0.247 0.217 0.237 0.043 0.126 0.178 0.162 0.185 0.172 0.189 0.136 0.094 0.078 0.174 0.233 0.15 0.078 0.129 0.135 0.178 0.107 0.064 0.185 0.145 0.114 0.124 0.129 0.147 0.124 0.089 0.064 0.173 0.223 0.075 0.019 0.088 0.036 0.028 0.055 2.86 0.042 0.081 0.017 0.039 91.4 180 93 107 143 376 163 192 249 109 0.382 0.224 2.05 0.78 0.261 0.334 0.544 0.605 0.234 0.306 7.73 9.14 6.78 7.03 11.8 8.65 16.8 13.2 18.7 11.4 13 18.5 14.3 19.8 11.9 11.9 13.4 7.65 8.39 9.02 10.6
Details Graph
Individual
Expts		Ave Biol. Reps
LM_ID Name 25-OHC*
*
25-OHC
Ctrl
 0 hrs		 25-OHC*
*
25-OHC
Ctrl
 30 min		 25-OHC*
*
25-OHC
Ctrl
 1 hr		 25-OHC*
*
25-OHC
Ctrl
 2 hrs		 25-OHC*
*
25-OHC
Ctrl
 4 hrs		 25-OHC*
*
25-OHC
Ctrl
 8 hrs		 25-OHC*
*
25-OHC
Ctrl
 12 hrs		 25-OHC*
*
25-OHC
Ctrl
 24 hrs		Units
. . . .
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060111)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ060718)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070123)		LMSP01050001 Sphingosine-P pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG070306)		LMSP01050001 Sphingosine-P pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG061213)		LMSP01050001 Sphingosine-P pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070619)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070515)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070515)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD070816)		LMSP01050001 Sphingosine-P pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080212)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080219)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ080226)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI081123)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI081130)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI081207)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090127)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090114)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090401)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP01050001 Sphingosine-P pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ081209)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090512)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090716)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD090805)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMSP01050001 Sphingosine-P -
0.01
-
0.01		 0.01
0.01
0.01
0.02		 0.01
0.01
0.01
0.02		 0.02
0.02
0.01
0.01		 0.02
0.02
0.02
0.01		 0.01
0.02
0.01
0.01		 0.01
0.02
0.01
0.01		 0.01
0.02
0.01
0.01		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		LMSP01050001 Sphingosine-P -
0.03
-
0.02		 0.02
0.02
0.02
0.01		 0.01
0.02
0.02
0.02		 0.03
0.02
0.01
0.02		 0.02
0.03
0.02
0.02		 0.02
0.02
0.01
0.01		 0.02
0.02
0.02
0.02		 0.02
0.02
0.01
0.02		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		LMSP01050001 Sphingosine-P -
0.04
-
0.04		 0.03
0.04
0.04
0.04		 0.04
0.04
0.05
0.04		 0.03
0.04
0.04
0.03		 0.04
0.04
0.05
0.05		 0.04
0.03
0.02
0.05		 0.04
0.06
0.03
0.03		 0.03
0.04
0.04
0.04		 pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100322)		LMSP01050001 Sphingosine-P -
0.1
-
0.1		 0.11
0.1
0.11
0.13		 0.12
0.12
0.11
0.22		 0.16
0.18
0.14
0.12		 0.14
0.16
0.16
0.09		 0.11
0.22
0.14
0.15		 0.1
0.17
0.14
0.15		 0.12
0.17
0.1
0.11		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100427)		LMSP01050001 Sphingosine-P -
0.22
-
0.19		 0.17
0.18
0.18
0.11		 0.09
0.2
0.2
0.23		 0.23
0.22
0.12
0.22		 0.16
0.2
0.19
0.14		 0.2
0.21
0.12
0.1		 0.17
0.19
0.21
0.28		 0.2
0.19
0.11
0.2		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD100624)		LMSP01050001 Sphingosine-P -
0.32
-
0.3		 0.22
0.23
0.24
0.28		 0.29
0.25
0.33
0.29		 0.21
0.26
0.23
0.23		 0.27
0.27
0.22
0.24		 0.27
0.2
0.15
0.28		 0.21
0.27
0.16
0.21		 0.16
0.2
0.19
0.21		 pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		LMSP01050001 Sphingosine-P pmol/sample
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD110605)		LMSP01050002 Sphingosine-P pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120120)		LMSP01050001 Sphingosine-P pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120131)		LMSP01050001 Sphingosine-P pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120131)		LMSP01050001 Sphingosine-P pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCG120308)		LMSP01050001 Sphingosine-P pmol/10E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0003)		LMSP01050001 Sphingosine-P pmol/mg
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0009)		LMSP01050001 Sphingosine-P pmol/ml
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(NASH0001)		LMSP01050001 Sphingosine-P pmol/ml
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060122)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060126)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCI060614)		LMSP01050001 Sphingosine-P pmol/ug DNA
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCD070717)		LMSP01050001 Sphingosine-P pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070122)		LMSP01050001 Sphingosine-P pmol/1E6 cells
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
 
Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp
(BCJ070124)		LMSP01050001 Sphingosine-P pmol/1E6 cells