Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: Stearic acid Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 10938.7 31.5 90.4 53 51.1 106 127 45 107 86.1 71.6 40.9 18.1 15.5 61.8 55 56 41.1 33.6 54.4 50.8 64.5 116 95.3 97.3 133 90.9 70.3 69.8 71.5 41.6 41.8 40.2 55 49.7 53.2 46.4 47.1 51.7 45.5 40 52.1 40.1 40 47.3 45.2 53.4 45.3 38.8 30.7 38.8 25 32.4 38.3 42.4 36.4 34.1 30 30.7 37.8 43.3 39 135 148 138 160 126 119 174 123 111 111 131 94.9 155 152 206 146 51 48.9 46.9 68.2 77.8 65.3 69 48.2 60.9 40.3 58.7 55.9 66.3 53.6 68.2 60.3 63.3 68.8 74.3 55.6 62.3 48.6 61.5 69.8 54.7 69.3 65.3 61.1 66.2 55.9 63.7 62.9 184 239 288 242 298 230 222 153 41.4 43.2 41.4 91.8 82.9 204 111 87.6 58.5 55.5 60.3 125 210 155 141 115 80.2 50.7 69.7 67.6 20.4 62 20.7 8.87 7.5 12.6 18.4 618 792 647 615 879 998 769 568 749 708 589 1470 221 419 561 548 572 453 - 124 174 4.08 380 173 113 28.1 0.785 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070804) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070612) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070804) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070814) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070817) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080227) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080520) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080520) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080702) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080702) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG080702) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090924) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090924) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG090928) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMFA01010018 Stearic acid - 36.3 - 27.8 33.4 43.8 23.1 60.6 21.2 32 29.3 31.8 30.6 40.2 19.4 17.6 34.9 32 54.6 27.5 28 31.4 26.8 18.5 38 38.1 27.7 27.1 31.6 43.2 27.5 36.5 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMFA01010018 Stearic acid - 111 - 59.1 58.1 69.9 60.8 56.1 65.3 63.4 61.5 39.3 68.5 48.1 48.7 39.5 77.5 82.7 55.5 78.6 77.4 65.7 45.3 97.8 79.1 59.7 59 46.9 110 110 64.7 78.5 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMFA01010018 Stearic acid - 85.3 - 132 111 155 147 111 95.9 141 151 115 127 117 123 102 102 116 215 151 136 132 128 143 239 238 201 110 262 223 207 164 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMFA01010018 Stearic acid - 355 - 284 377 455 260 405 260 378 312 354 242 357 279 215 250 263 424 249 305 350 365 279 375 316 381 407 372 370 280 410 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100427) LMFA01010018 Stearic acid - 821 - 556 480 619 540 611 605 631 610 456 526 537 595 428 601 546 519 557 777 701 540 1015 667 578 633 650 1099 1016 694 789 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100624) LMFA01010018 Stearic acid - 683 - 984 803 894 868 790 704 877 985 841 884 777 700 795 695 789 963 713 920 881 970 801 1236 1090 1083 777 1401 1089 959 848 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120308) LMFA01010018 Stearic acid pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070831) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070927) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070830) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070924) LMFA01010018 Stearic acid pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070919) LMFA01010018 Stearic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070815) LMFA01010018 Stearic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070316) LMFA01010018 Stearic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070316) LMFA01010018 Stearic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSG070316) LMFA01010018 Stearic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070717) LMFA01010018 Stearic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD070815) LMFA01010018 Stearic acid pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120120) LMFA01010018 Stearic acid pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120131) LMFA01010018 Stearic acid pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120212) LMFA01010018 Stearic acid pmol/10E6 cells