Lipidomics studies on macrophages - bone marrow-derived macrophages treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

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Details: Zymosterol Legend: : View detailed data for replicates: View time course graphs Ctrl: Untreated BMDM cells (control) 25-OHC: Cells treated with 25-OH-cholesterol at time 0 *: BMDM cells in lipid-depleted media containing compactin 25-OHC*: cells in lipid-depleted media containing compactin and treated with 25-OHC at time 0 2.222.1 2.72 4.2 1.53 1.23 1.34 2.57 1.36 9.19 13 11.3 12.5 11.8 13.7 11.8 5.72 2.52 6.96 4.6 5.72 5.14 5.58 5.5 5.71 6.81 1.29 6.61 11 6.09 5.79 5.35 6.52 7.57 3.67 1.14 8.36 6.08 4.39 - - - - - - - - - - - - - 1.34 - 0.455 - - 19.3 6.77 1.3 25.2 14.8 152 12.2 10.9 159 6.51 14.1 2.08 27.6 11.2 8.33 2.6 6.24 82.7 Details Graph Individual Expts Ave Biol. Reps LM_ID Name 25-OHC* * 25-OHC Ctrl 0 hrs 25-OHC* * 25-OHC Ctrl 30 min 25-OHC* * 25-OHC Ctrl 1 hr 25-OHC* * 25-OHC Ctrl 2 hrs 25-OHC* * 25-OHC Ctrl 4 hrs 25-OHC* * 25-OHC Ctrl 8 hrs 25-OHC* * 25-OHC Ctrl 12 hrs 25-OHC* * 25-OHC Ctrl 24 hrs Units . . . . Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010066 Zymosterol - 0.01 - 0.02 0.02 - 1 0.86 0.01 - 0.78 0.72 0.01 0.01 0.34 0.67 0.01 0.01 0.27 1.19 - 0.01 0.2 0.63 - 0.01 0.15 0.7 - - 0.01 0.64 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010066 Zymosterol - 0.01 - 1.86 0.14 2.38 0.09 2 0.07 0.17 1.98 1.94 0.03 0.04 1.62 1.94 0.08 0.11 1.26 2.96 0.03 0.06 0.35 2.63 - 0.23 0.42 1.59 0.04 0.07 0.26 1.66 pmol/ug DNA Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010066 Zymosterol - 0.1 - 0.2 0.23 - 11.2 5.74 0.12 - 8.31 8.02 0.08 0.09 4.88 8.2 0.07 0.08 2.09 10.8 - 0.11 2.72 9.53 - 0.08 2.06 10.5 - - 0.1 7.18 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD100322) LMST01010066 Zymosterol - 0.1 - 19 1.58 24.7 1.01 13.3 0.86 2.01 21.1 21.6 0.24 0.36 23.3 23.8 0.57 0.91 9.77 26.8 0.33 0.67 4.76 39.8 - 1.91 5.78 23.9 0.47 0.6 2.64 18.6 pmol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (MSJ110501) LMST01010066 Zymosterol pmol/1E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMST01010066 Zymosterol umol/sample Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) LMST01010066 Zymosterol umol/ml of media Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCD110605) Zymosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120120) LMST01010066 Zymosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120131) LMST01010066 Zymosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120212) LMST01010066 Zymosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (BCG120308) LMST01010066 Zymosterol pmol/10E6 cells Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0001) LMST01010066 Zymosterol pmol/mg Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp   Legend: :Ctrl  :25-OHC  :Comp  :25-OHC + Comp (NASH0018) LMST01010066 Zymosterol pmol/ml