Gene/Proteome Database (LMPD)
Proteins
| phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform 1 | |
|---|---|
| Refseq ID | NP_001280636 |
| Protein GI | 656985036 |
| UniProt ID | P70182 |
| mRNA ID | NM_001293707 |
| Length | 547 |
| RefSeq Status | VALIDATED |
| MASASSGPAAAGFSSLDAGAPAGTAAAASGIKRATVSEGPSASVMPVKKIGHRSVDSSGETTYKKTTSSALKGAIQLGITHTVGSLSTKPERDVLMQDFYVVESIFFPSEGSNLTPAHHYNDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCSEPLIELSNSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNIRIVVMNNLLPRSVKMHMKYDLKGSTYKRRASQKEREKTLPTFKDLDFLQDIPDGLFLDADMYSALCKTLQRDCLVLQSFKIMDYSLLMSIHNMDHAQREPTSNDTQYSADTRRPAPQKALYSTAMESIQGEARRGGTVETEDHMGGIPARNNKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPLKPSPTKKFRSGPSFSRRSGPSGNSCTSQLMASGEHRAQVTTKAEVEPDVHLGRPDVLPQTPPLEEISEGSPVPGPSFSPVVGQPLQILNLSSTLEKLDVAESEFTH | |
| phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform 2 | |
|---|---|
| Refseq ID | NP_032873 |
| Protein GI | 31982833 |
| UniProt ID | P70182 |
| mRNA ID | NM_008847 |
| Length | 546 |
| RefSeq Status | VALIDATED |
| MASASSGPAAAGFSSLDAGAPAGTAAASGIKRATVSEGPSASVMPVKKIGHRSVDSSGETTYKKTTSSALKGAIQLGITHTVGSLSTKPERDVLMQDFYVVESIFFPSEGSNLTPAHHYNDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCSEPLIELSNSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNIRIVVMNNLLPRSVKMHMKYDLKGSTYKRRASQKEREKTLPTFKDLDFLQDIPDGLFLDADMYSALCKTLQRDCLVLQSFKIMDYSLLMSIHNMDHAQREPTSNDTQYSADTRRPAPQKALYSTAMESIQGEARRGGTVETEDHMGGIPARNNKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPLKPSPTKKFRSGPSFSRRSGPSGNSCTSQLMASGEHRAQVTTKAEVEPDVHLGRPDVLPQTPPLEEISEGSPVPGPSFSPVVGQPLQILNLSSTLEKLDVAESEFTH | |
Gene Information
Entrez Gene ID
Gene Name
phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha
Gene Symbol
Species
Mus musculus
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IEA:UniProtKB-KW | C | cytoplasm |
| GO:0030027 | IEA:Ensembl | C | lamellipodium |
| GO:0005847 | IEA:Ensembl | C | mRNA cleavage and polyadenylation specificity factor complex |
| GO:0016607 | ISS:UniProtKB | C | nuclear speck |
| GO:0005634 | IDA:MGI | C | nucleus |
| GO:0005886 | ISS:UniProtKB | C | plasma membrane |
| GO:0032587 | IDA:MGI | C | ruffle membrane |
| GO:0016308 | IDA:MGI | F | 1-phosphatidylinositol-4-phosphate 5-kinase activity |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0031532 | ISS:UniProtKB | P | actin cytoskeleton reorganization |
| GO:0032863 | ISS:UniProtKB | P | activation of Rac GTPase activity |
| GO:0060326 | ISS:UniProtKB | P | cell chemotaxis |
| GO:0010761 | IMP:MGI | P | fibroblast migration |
| GO:0048041 | ISS:UniProtKB | P | focal adhesion assembly |
| GO:0046488 | IDA:MGI | P | phosphatidylinositol metabolic process |
| GO:0046854 | IDA:GOC | P | phosphatidylinositol phosphorylation |
| GO:0008654 | IEA:Ensembl | P | phospholipid biosynthetic process |
| GO:0072661 | ISS:UniProtKB | P | protein targeting to plasma membrane |
| GO:0097178 | ISS:UniProtKB | P | ruffle assembly |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko04144 | Endocytosis |
| mmu04144 | Endocytosis |
| ko04666 | Fc gamma R-mediated phagocytosis |
| mmu04666 | Fc gamma R-mediated phagocytosis |
| ko00562 | Inositol phosphate metabolism |
| mmu00562 | Inositol phosphate metabolism |
| M00130 | Inositol phosphate metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 |
| mmu01100 | Metabolic pathways |
| ko04070 | Phosphatidylinositol signaling system |
| mmu04070 | Phosphatidylinositol signaling system |
| ko04810 | Regulation of actin cytoskeleton |
| mmu04810 | Regulation of actin cytoskeleton |
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| PWY-6352 | 3-phosphoinositide biosynthesis |
| PWY-6351 | D-myo-inositol (1,4,5)-trisphosphate biosynthesis |
| PWY3DJ-219 | PIP metabolism |
REACTOME Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR023610 | Phosphatidylinositol-4-phosphate 5-kinase |
| IPR002498 | Phosphatidylinositol-4-phosphate 5-kinase, core |
| IPR016034 | Phosphatidylinositol-4-phosphate 5-kinase, core, subgroup |
| IPR027483 | Phosphatidylinositol-4-phosphate 5-kinase, C-terminal |
| IPR027484 | Phosphatidylinositol-4-phosphate 5-kinase, N-terminal domain |
UniProt Annotations
Entry Information
Gene Name
phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha
Protein Entry
P70182_MOUSE
UniProt ID
Species
Mouse
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=P70182-1; Sequence=Displayed; Name=2; IsoId=P70182-2; Sequence=VSP_053437, VSP_016009; Note=No experimental confirmation available.; Name=3; IsoId=P70182-3; Sequence=VSP_053437; Note=No experimental confirmation available.; |
| Biophysicochemical Properties | Kinetic parameters: KM=26 uM for PtdIns(4)P {ECO:0000269|PubMed:9535851}; KM=33 uM for ATP {ECO:0000269|PubMed:9535851}; |
| Catalytic Activity | ATP + 1-phosphatidyl-1D-myo-inositol 4- phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. |
| Caution | There is confusion in the literature with phosphatidylinositol 4-phosphate 5-kinase type I nomenclature due to the fact that frequently mouse PIP5K1B is named Phosphatidylinositol 4-phosphate 5-kinase type I alpha. {ECO:0000305}. |
| Developmental Stage | Expression is highest during early embryogenesis and slightly decreases over time. {ECO:0000269|PubMed:20622009}. |
| Disruption Phenotype | Survive to adulthood, but bred poorly and display reduced fertility. Failed to form any vessel occlusion after chemical-induced carotid injury. Platelets have defective aggregation. Bone marrow-derived macrophages are defective in actin polymerization during phagocytosis. PIP5K1A and PIP5K1C double mutant mice are embryonic lethal. {ECO:0000269|PubMed:18772378, ECO:0000269|PubMed:19153220, ECO:0000269|PubMed:20622009}. |
| Enzyme Regulation | Activated by phosphatidic acid. {ECO:0000269|PubMed:8798574, ECO:0000269|PubMed:9535851}. |
| Function | Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. {ECO:0000269|PubMed:10679324, ECO:0000269|PubMed:18772378, ECO:0000269|PubMed:19153220, ECO:0000269|PubMed:20622009, ECO:0000269|PubMed:8798574}. |
| Similarity | Contains 1 PIPK domain. {ECO:0000255|PROSITE- ProRule:PRU00781}. |
| Subcellular Location | Cell membrane {ECO:0000269|PubMed:20622009}. Nucleus speckle {ECO:0000250}. Cytoplasm {ECO:0000269|PubMed:20622009}. Cell projection, ruffle {ECO:0000250}. Note=Colocalizes with RAC1 at actin-rich membrane ruffles. Localizes to nuclear speckles and associates with TUT1 to regulate polyadenylation of selected mRNAs. {ECO:0000250}. |
| Subunit | Interacts with RAC1. Interacts with TUT1 (By similarity). Forms a complex with CDH1/E-cadherin, CTNNB1/beta-catenin and CTNND1 at the plasma membrane upon calcium stimulation (By similarity). {ECO:0000250}. |
| Tissue Specificity | Highest expression in brain. Also detected in skeletal muscle, testis, brain and lung. {ECO:0000269|PubMed:20622009, ECO:0000269|PubMed:8798574}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP000013 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 656985036 | RefSeq | NP_001280636 | 547 | phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform 1 |
| 31982833 | RefSeq | NP_032873 | 546 | phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform 2 |
Identical Sequences to LMP000013 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:31982833 | DBBJ | BAE34344.1 | 546 | unnamed protein product [Mus musculus] |
| GI:31982833 | GenBank | AAH31774.1 | 546 | Phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha [Mus musculus] |
| GI:656985036 | GenBank | ABK42321.1 | 547 | PI(4)P5-K beta [synthetic construct] |
| GI:31982833 | GenBank | EDL38772.1 | 546 | phosphatidylinositol-4-phosphate 5-kinase, type 1 beta [Mus musculus] |
| GI:31982833 | SwissProt | P70182.2 | 546 | RecName: Full=Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; Short=PIP5K1-alpha; Short=PtdIns(4)P-5-kinase 1 alpha; AltName: Full=68 kDa type I phosphatidylinositol 4-phosphate 5-kinase; AltName: Full=Phosphatidylinositol 4-phosphate 5-kinase type I alpha; Short=PIP5KIalpha; AltName: Full=Phosphatidylinositol 4-phosphate 5-kinase type I beta; Short=PI4P5KIbeta [Mus musculus] |
Related Sequences to LMP000013 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:31982833 | DBBJ | BAA13031.1 | 546 | phosphatidylinositol 4-phosphate 5-kinase type I-beta [Mus musculus] |
| GI:656985036 | DBBJ | BAE29943.1 | 547 | unnamed protein product [Mus musculus] |
| GI:31982833 | DBBJ | BAE29943.1 | 547 | unnamed protein product [Mus musculus] |
| GI:656985036 | DBBJ | BAE34344.1 | 546 | unnamed protein product [Mus musculus] |
| GI:656985036 | DBBJ | BAE30850.1 | 547 | unnamed protein product [Mus musculus] |
| GI:31982833 | DBBJ | BAE30850.1 | 547 | unnamed protein product [Mus musculus] |
| GI:656985036 | GenBank | AAH31774.1 | 546 | Phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha [Mus musculus] |
| GI:31982833 | GenBank | ABK42321.1 | 547 | PI(4)P5-K beta [synthetic construct] |
| GI:656985036 | GenBank | EDL38772.1 | 546 | phosphatidylinositol-4-phosphate 5-kinase, type 1 beta [Mus musculus] |
| GI:31982833 | RefSeq | NP_001280636.1 | 547 | phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform 1 [Mus musculus] |
| GI:31982833 | RefSeq | XP_008759537.1 | 548 | PREDICTED: phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform X1 [Rattus norvegicus] |
| GI:656985036 | SwissProt | P70182.2 | 546 | RecName: Full=Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; Short=PIP5K1-alpha; Short=PtdIns(4)P-5-kinase 1 alpha; AltName: Full=68 kDa type I phosphatidylinositol 4-phosphate 5-kinase; AltName: Full=Phosphatidylinositol 4-phosphate 5-kinase type I alpha; Short=PIP5KIalpha; AltName: Full=Phosphatidylinositol 4-phosphate 5-kinase type I beta; Short=PI4P5KIbeta [Mus musculus] |