Gene/Proteome Database (LMPD)

LMPD ID
LMP000300
Gene ID
11806
Species
Mus musculus (Mouse)
Gene Name
apolipoprotein A-I
Gene Symbol
Apoa1
Synonyms
Alp-1; Apoa-1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2; apo-AI; apoA-I
Alternate Names
apolipoprotein A-I; apolipoprotein A1
Chromosome
9
Map Location
9 A2-A4|9 25.36 cM
Summary
This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is the major protein component of plasma high density lipoprotein (HDL). This protein facilitates the removal of cholesterol and other fats from tissues by transporting them to the liver for excretion. This protein is a cofactor for lecithin cholesterolacyltransferase, an enzyme that catalyzes the conversion of free cholesterol to cholesteryl esters. Mutations in this gene in humans causes familial HDL deficiency, Tangier disease and familial visceral amyloidosis. Similar clinical features are exhibited by mice with mutations in this gene. This gene is clustered with three other apolipoprotein genes on chromosome 9. [provided by RefSeq, Dec 2013]
Orthologs
View orthologs and multiple alignments for Apoa1

Proteins

apolipoprotein A-I preproprotein
Refseq ID NP_033822
Protein GI 160333304
UniProt ID Q00623
mRNA ID NM_009692
Length 264
RefSeq Status REVIEWED
MKAVVLAVALVFLTGSQAWHVWQQDEPQSQWDKVKDFANVYVDAVKDSGRDYVSQFESSSLGQQLNLNLLENWDTLGSTVSQLQERLGPLTRDFWDNLEKETDWVRQEMNKDLEEVKQKVQPYLDEFQKKWKEDVELYRQKVAPLGAELQESARQKLQELQGRLSPVAEEFRDRMRTHVDSLRTQLAPHSEQMRESLAQRLAELKSNPTLNEYHTRAKTHLKTLGEKARPALEDLRHSLMPMLETLKTQVQSVIDKASETLTAQ
sig_peptide: 1..18 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 1818 peptide sequence: MKAVVLAVALVFLTGSQA mat_peptide: 19..264 product: Proapolipoprotein A-I experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q00623.2) calculated_mol_wt: 28815 peptide sequence: WHVWQQDEPQSQWDKVKDFANVYVDAVKDSGRDYVSQFESSSLGQQLNLNLLENWDTLGSTVSQLQERLGPLTRDFWDNLEKETDWVRQEMNKDLEEVKQKVQPYLDEFQKKWKEDVELYRQKVAPLGAELQESARQKLQELQGRLSPVAEEFRDRMRTHVDSLRTQLAPHSEQMRESLAQRLAELKSNPTLNEYHTRAKTHLKTLGEKARPALEDLRHSLMPMLETLKTQVQSVIDKASETLTAQ mat_peptide: 25..264 product: Apolipoprotein A-I experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q00623.2) calculated_mol_wt: 27950 peptide sequence: DEPQSQWDKVKDFANVYVDAVKDSGRDYVSQFESSSLGQQLNLNLLENWDTLGSTVSQLQERLGPLTRDFWDNLEKETDWVRQEMNKDLEEVKQKVQPYLDEFQKKWKEDVELYRQKVAPLGAELQESARQKLQELQGRLSPVAEEFRDRMRTHVDSLRTQLAPHSEQMRESLAQRLAELKSNPTLNEYHTRAKTHLKTLGEKARPALEDLRHSLMPMLETLKTQVQSVIDKASETLTAQ mat_peptide: 25..263 product: Truncated apolipoprotein A-I (By similarity) inference: non-experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q00623.2) calculated_mol_wt: 27822 peptide sequence: DEPQSQWDKVKDFANVYVDAVKDSGRDYVSQFESSSLGQQLNLNLLENWDTLGSTVSQLQERLGPLTRDFWDNLEKETDWVRQEMNKDLEEVKQKVQPYLDEFQKKWKEDVELYRQKVAPLGAELQESARQKLQELQGRLSPVAEEFRDRMRTHVDSLRTQLAPHSEQMRESLAQRLAELKSNPTLNEYHTRAKTHLKTLGEKARPALEDLRHSLMPMLETLKTQVQSVIDKASETLTA

Gene Information

Entrez Gene ID
11806
Gene Name
apolipoprotein A-I
Gene Symbol
Apoa1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0072562 IEA:Ensembl C blood microparticle
GO:0005829 TAS:Reactome C cytosol
GO:0030139 IEA:Ensembl C endocytic vesicle
GO:0005576 IDA:MGI C extracellular region
GO:0005615 IDA:MGI C extracellular space
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0005634 IEA:Ensembl C nucleus
GO:0034366 IEA:Ensembl C spherical high-density lipoprotein particle
GO:0034361 IEA:Ensembl C very-low-density lipoprotein particle
GO:0001540 IEA:Ensembl F beta-amyloid binding
GO:0015485 IEA:Ensembl F cholesterol binding
GO:0017127 IDA:MGI F cholesterol transporter activity
GO:0008035 IDA:MGI F high-density lipoprotein particle binding
GO:0042802 IPI:MGI F identical protein binding
GO:0055102 IEA:Ensembl F lipase inhibitor activity
GO:0008289 IDA:MGI F lipid binding
GO:0005319 IDA:MGI F lipid transporter activity
GO:0071813 IDA:MGI F lipoprotein particle binding
GO:0060228 IEA:Ensembl F phosphatidylcholine-sterol O-acyltransferase activator activity
GO:0005543 IEA:Ensembl F phospholipid binding
GO:0005548 IEA:Ensembl F phospholipid transporter activity
GO:0007186 IEA:Ensembl P G-protein coupled receptor signaling pathway
GO:0030325 IMP:MGI P adrenal gland development
GO:0043534 IMP:MGI P blood vessel endothelial cell migration
GO:0006695 IMP:MGI P cholesterol biosynthetic process
GO:0033344 IDA:MGI P cholesterol efflux
GO:0042632 IEA:Ensembl P cholesterol homeostasis
GO:0070508 IEA:Ensembl P cholesterol import
GO:0008203 IMP:MGI P cholesterol metabolic process
GO:0030301 IDA:MGI P cholesterol transport
GO:0001935 IMP:MGI P endothelial cell proliferation
GO:0008211 IMP:MGI P glucocorticoid metabolic process
GO:0034380 IEA:Ensembl P high-density lipoprotein particle assembly
GO:0019915 IMP:MGI P lipid storage
GO:0042158 IMP:MGI P lipoprotein biosynthetic process
GO:0060354 IEA:Ensembl P negative regulation of cell adhesion molecule production
GO:0002740 IEA:Ensembl P negative regulation of cytokine secretion involved in immune response
GO:0034115 IEA:Ensembl P negative regulation of heterotypic cell-cell adhesion
GO:0051346 IGI:MGI P negative regulation of hydrolase activity
GO:0050728 IEA:Ensembl P negative regulation of inflammatory response
GO:0050713 IEA:Ensembl P negative regulation of interleukin-1 beta secretion
GO:0060192 IEA:Ensembl P negative regulation of lipase activity
GO:0010804 IEA:Ensembl P negative regulation of tumor necrosis factor-mediated signaling pathway
GO:0010903 IEA:Ensembl P negative regulation of very-low-density lipoprotein particle remodeling
GO:0031100 IEA:Ensembl P organ regeneration
GO:0018206 ISS:UniProtKB P peptidyl-methionine modification
GO:0006656 IEA:Ensembl P phosphatidylcholine biosynthetic process
GO:0033700 IEA:Ensembl P phospholipid efflux
GO:0055091 IEA:Ensembl P phospholipid homeostasis
GO:0006644 IMP:MGI P phospholipid metabolic process
GO:0010873 IEA:Ensembl P positive regulation of cholesterol esterification
GO:0051345 IEA:Ensembl P positive regulation of hydrolase activity
GO:0051347 IMP:MGI P positive regulation of transferase activity
GO:0018158 ISS:UniProtKB P protein oxidation
GO:0050821 IEA:Ensembl P protein stabilization
GO:0032489 IEA:Ensembl P regulation of Cdc42 protein signal transduction
GO:0030300 IDA:MGI P regulation of intestinal cholesterol absorption
GO:0001932 IDA:MGI P regulation of protein phosphorylation
GO:0042493 IEA:Ensembl P response to drug
GO:0043627 IEA:Ensembl P response to estrogen
GO:0007584 IEA:Ensembl P response to nutrient
GO:0043691 IEA:Ensembl P reverse cholesterol transport
GO:0070328 IEA:Ensembl P triglyceride homeostasis

KEGG Pathway Links

KEGG Pathway ID Description
mmu05143 African trypanosomiasis
mmu04975 Fat digestion and absorption
mmu04977 Vitamin digestion and absorption

Domain Information

InterPro Annotations

Accession Description
IPR000074 Apolipoprotein A/E

UniProt Annotations

Entry Information

Gene Name
apolipoprotein A-I
Protein Entry
APOA1_MOUSE
UniProt ID
Q00623
Species
Mouse

Comments

Comment Type Description
Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Interaction Q08460:Kcnma1; NbExp=4; IntAct=EBI-1634106, EBI-1633915;
Mass Spectrometry Mass=27923.8; Method=Electrospray; Range=25- 264; Note=Strain BALB/c. Without methionine sulfoxide.; Evidence={ECO:0000269|PubMed:16876491};
Mass Spectrometry Mass=27951.3; Mass_error=1.343; Method=Electrospray; Range=25-264; Note=Strain C57BL/6. Without methionine sulfoxide.; Evidence={ECO:0000269|PubMed:16876491};
Mass Spectrometry Mass=27965; Method=Electrospray; Range=25-264; Note=Strain C57BL/6. With 1 methionine sulfoxide.; Evidence={ECO:0000269|PubMed:16876491};
Mass Spectrometry Mass=28790.7; Method=Electrospray; Range=19- 264; Note=Strain BALB/c. Without methionine sulfoxide.; Evidence={ECO:0000269|PubMed:16876491};
Mass Spectrometry Mass=28819.7; Method=Electrospray; Range=19- 264; Note=Strain C57BL/6. Without methionine sulfoxide.; Evidence={ECO:0000269|PubMed:16876491};
Ptm Glycosylated. {ECO:0000250}.
Ptm May be acylated.
Ptm Palmitoylated. {ECO:0000250}.
Ptm Phosphorylation sites are present in the extracellular medium. {ECO:0000250}.
Similarity Belongs to the apolipoprotein A1/A4/E family. {ECO:0000305}.
Subcellular Location Secreted.
Subunit Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1 (By similarity). {ECO:0000250}.
Tissue Specificity Major protein of plasma HDL, also found in chylomicrons.

Identical and Related Proteins

Unique RefSeq proteins for LMP000300 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
160333304 RefSeq NP_033822 264 apolipoprotein A-I preproprotein

Identical Sequences to LMP000300 proteins

Reference Database Accession Length Protein Name
GI:160333304 DBBJ BAC36241.1 264 unnamed protein product [Mus musculus]
GI:160333304 DBBJ BAE28968.1 264 unnamed protein product [Mus musculus]
GI:160333304 DBBJ BAE36448.1 264 unnamed protein product [Mus musculus]
GI:160333304 SwissProt Q00623.2 264 RecName: Full=Apolipoprotein A-I; Short=Apo-AI; Short=ApoA-I; AltName: Full=Apolipoprotein A1; Contains: RecName: Full=Proapolipoprotein A-I; Short=ProapoA-I; Contains: RecName: Full=Truncated apolipoprotein A-I; Flags: Precursor [Mus musculus]

Related Sequences to LMP000300 proteins

Reference Database Accession Length Protein Name
GI:160333304 DBBJ BAE20837.1 264 unnamed protein product [Mus musculus]
GI:160333304 EMBL CAA45560.1 264 precursor Apoprotein A-I [Mus musculus]
GI:160333304 EMBL CAA45561.1 264 Apolipoprotein A-I [Mus musculus]
GI:160333304 GenBank AAH12253.1 264 Apolipoprotein A-I [Mus musculus]
GI:160333304 GenBank AAH19837.1 264 Apolipoprotein A-I [Mus musculus]
GI:160333304 GenBank EDL25679.1 264 apolipoprotein A-I, isoform CRA_c [Mus musculus]