Gene/Proteome Database (LMPD)

LMPD ID
LMP000303
Gene ID
3633
Species
Homo sapiens (Human)
Gene Name
inositol polyphosphate-5-phosphatase, 75kDa
Gene Symbol
INPP5B
Synonyms
5PTase
Chromosome
1
Map Location
1p34
EC Number
3.1.3.36
Summary
This gene encodes a member of a family of inositol polyphosphate-5-phosphatases. These enzymes function in the regulation of calcium signaling by inactivating inositol phosphates. The encoded protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jul 2014]
Orthologs
View orthologs and multiple alignments for INPP5B

Proteins

type II inositol 1,4,5-trisphosphate 5-phosphatase isoform 1 precursor
Refseq ID NP_005531
Protein GI 113722125
UniProt ID P32019
mRNA ID NM_005540
Length 913
RefSeq Status REVIEWED
MDQSVAIQETLAEGEYCVIAVQGVLCEGDSRQSRLLGLVRYRLEHGGQEHALFLYTHRRMAITGDDVSLDQIVPVSRDFTLEEVSPDGELYILGSDVTVQLDTAELSLVFQLPFGSQTRMFLHEVARACPGFDSATRDPEFLWLSRYRCAELELEMPTPRGCNSALVTWPGYATIGGGGSNFDGLRPNGKGVPMDQSSRGQDKPESLQPRQNKSKSEITDMVRSSTITVSDKAHILSMQKFGLRDTIVKSHLLQKEEDYTYIQNFRFFAGTYNVNGQSPKECLRLWLSNGIQAPDVYCVGFQELDLSKEAFFFHDTPKEEEWFKAVSEGLHPDAKYAKVKLIRLVGIMLLLYVKQEHAAYISEVEAETVGTGIMGRMGNKGGVAIRFQFHNTSICVVNSHLAAHIEEYERRNQDYKDICSRMQFCQPDPSLPPLTISNHDVILWLGDLNYRIEELDVEKVKKLIEEKDFQMLYAYDQLKIQVAAKTVFEGFTEGELTFQPTYKYDTGSDDWDTSEKCRAPAWCDRILWKGKNITQLSYQSHMALKTSDHKPVSSVFDIGVRVVNDELYRKTLEEIVRSLDKMENANIPSVSLSKREFCFQNVKYMQLKVESFTIHNGQVPCHFEFINKPDEESYCKQWLNANPSRGFLLPDSDVEIDLELFVNKMTATKLNSGEDKIEDILVLHLDRGKDYFLSVSGNYLPSCFGSPIHTLCYMREPILDLPLETISELTLMPVWTGDDGSQLDSPMEIPKELWMMVDYLYRNAVQQEDLFQQPGLRSEFEHIRDCLDTGMIDNLSASNHSVAEALLLFLESLPEPVICYSTYHNCLECSGNYTASKQVISTLPIFHKNVFHYLMAFLRELLKNSAKNHLDENILASIFGSLLLRNPAGHQKLDMTEKKKAQEFIHQFLCNPL
mat_peptide: 241..913 product: type II inositol 1,4,5-trisphosphate 5-phosphatase isoform 1 calculated_mol_wt: 77477 peptide sequence: FGLRDTIVKSHLLQKEEDYTYIQNFRFFAGTYNVNGQSPKECLRLWLSNGIQAPDVYCVGFQELDLSKEAFFFHDTPKEEEWFKAVSEGLHPDAKYAKVKLIRLVGIMLLLYVKQEHAAYISEVEAETVGTGIMGRMGNKGGVAIRFQFHNTSICVVNSHLAAHIEEYERRNQDYKDICSRMQFCQPDPSLPPLTISNHDVILWLGDLNYRIEELDVEKVKKLIEEKDFQMLYAYDQLKIQVAAKTVFEGFTEGELTFQPTYKYDTGSDDWDTSEKCRAPAWCDRILWKGKNITQLSYQSHMALKTSDHKPVSSVFDIGVRVVNDELYRKTLEEIVRSLDKMENANIPSVSLSKREFCFQNVKYMQLKVESFTIHNGQVPCHFEFINKPDEESYCKQWLNANPSRGFLLPDSDVEIDLELFVNKMTATKLNSGEDKIEDILVLHLDRGKDYFLSVSGNYLPSCFGSPIHTLCYMREPILDLPLETISELTLMPVWTGDDGSQLDSPMEIPKELWMMVDYLYRNAVQQEDLFQQPGLRSEFEHIRDCLDTGMIDNLSASNHSVAEALLLFLESLPEPVICYSTYHNCLECSGNYTASKQVISTLPIFHKNVFHYLMAFLRELLKNSAKNHLDENILASIFGSLLLRNPAGHQKLDMTEKKKAQEFIHQFLCNPL
type II inositol 1,4,5-trisphosphate 5-phosphatase isoform 2 precursor
Refseq ID NP_001284363
Protein GI 661903016
UniProt ID P32019
mRNA ID NM_001297434
Length 749
RefSeq Status REVIEWED
MSAAAGSRERNTAGGSNFDGLRPNGKGVPMDQSSRGQDKPESLQPRQNKSKSEITDMVRSSTITVSDKAHILSMQKFGLRDTIVKSHLLQKEEDYTYIQNFRFFAGTYNVNGQSPKECLRLWLSNGIQAPDVYCVGFQELDLSKEAFFFHDTPKEEEWFKAVSEGLHPDAKYAKVKLIRLVGIMLLLYVKQEHAAYISEVEAETVGTGIMGRMGNKGGVAIRFQFHNTSICVVNSHLAAHIEEYERRNQDYKDICSRMQFCQPDPSLPPLTISNHDVILWLGDLNYRIEELDVEKVKKLIEEKDFQMLYAYDQLKIQVAAKTVFEGFTEGELTFQPTYKYDTGSDDWDTSEKCRAPAWCDRILWKGKNITQLSYQSHMALKTSDHKPVSSVFDIGVRVVNDELYRKTLEEIVRSLDKMENANIPSVSLSKREFCFQNVKYMQLKVESFTIHNGQVPCHFEFINKPDEESYCKQWLNANPSRGFLLPDSDVEIDLELFVNKMTATKLNSGEDKIEDILVLHLDRGKDYFLSVSGNYLPSCFGSPIHTLCYMREPILDLPLETISELTLMPVWTGDDGSQLDSPMEIPKELWMMVDYLYRNAVQQEDLFQQPGLRSEFEHIRDCLDTGMIDNLSASNHSVAEALLLFLESLPEPVICYSTYHNCLECSGNYTASKQVISTLPIFHKNVFHYLMAFLRELLKNSAKNHLDENILASIFGSLLLRNPAGHQKLDMTEKKKAQEFIHQFLCNPL

Gene Information

Entrez Gene ID
3633
Gene Name
inositol polyphosphate-5-phosphatase, 75kDa
Gene Symbol
INPP5B
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:HPA C cytoplasm
GO:0031410 IEA:UniProtKB-KW C cytoplasmic vesicle
GO:0005829 IDA:UniProtKB C cytosol
GO:0005768 IEA:UniProtKB-KW C endosome
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0016021 IEA:Ensembl C integral component of membrane
GO:0016020 IDA:UniProtKB C membrane
GO:0015630 IDA:HPA C microtubule cytoskeleton
GO:0005886 TAS:Reactome C plasma membrane
GO:0052658 IDA:UniProtKB F inositol-1,4,5-trisphosphate 5-phosphatase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0004439 IDA:UniProtKB F phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity
GO:0043647 TAS:Reactome P inositol phosphate metabolic process
GO:0001701 IEA:Ensembl P in utero embryonic development
GO:0046856 IDA:UniProtKB P phosphatidylinositol dephosphorylation
GO:0070613 IEA:Ensembl P regulation of protein processing
GO:0051056 TAS:Reactome P regulation of small GTPase mediated signal transduction
GO:0007264 TAS:Reactome P small GTPase mediated signal transduction
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0007283 IEA:Ensembl P spermatogenesis
GO:0030317 IEA:Ensembl P sperm motility

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6362 1D-myo-inositol hexakisphosphate biosynthesis II (mammalian)
PWY-6364 D-myo-inositol (1,3,4)-trisphosphate biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_150352 Synthesis of IP2, IP, and Ins in the cytosol
REACT_150312 Synthesis of IP3 and IP4 in the cytosol

Domain Information

InterPro Annotations

Accession Description
IPR005135 Endonuclease/exonuclease/phosphatase
IPR000300 Inositol polyphosphate-related phosphatase
IPR000198 Rho GTPase-activating protein domain
IPR008936 Rho GTPase activation protein

UniProt Annotations

Entry Information

Gene Name
inositol polyphosphate-5-phosphatase, 75kDa
Protein Entry
B3KMW4_HUMAN
UniProt ID
P32019
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=4; Name=1; IsoId=P32019-1; Sequence=Displayed; Note=No experimental confirmation available.; Name=2; IsoId=P32019-2; Sequence=VSP_012820; Name=3; IsoId=P32019-3; Sequence=VSP_012821; Name=4; IsoId=P32019-4; Sequence=VSP_013902, VSP_013903; Note=No experimental confirmation available.;
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Domain The ASH (ASPM-SPD2-Hydin) and RhoGAP (Rho GTPase activating) domains form a single folding module. The ASH domain has an immunoglobulin-like fold, the Rho-GAP domain lacks the catalytic arginine and is catalytically inactive. The ASH-RhoGAP module regulates the majority of the protein-protein interactions currently described. The ASH domain mediates association with membrane-targeting Rab GTPases. The Rho-GAP domain interacts with the endocytic adapter APPL1, which is then displaced by FAM109A and FAM109B as endosomes mature, all three interactions relie on F&H motifs, an approximately 12-13 amino-acid sequence centered around Phe and His residues essential for binding (By similarity).
Function Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events.
Ptm Isoprenylation at Cys-990 may be required for localization at the membrane.
Ptm May be proteolytically cleaved after Lys-320 as inferred from N-terminal protein sequence of the 75 kda form.
Sequence Caution Sequence=AAA79207.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence= ;
Similarity Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase type II family.
Similarity Contains 1 PH domain.
Similarity Contains 1 Rho-GAP domain. {ECO
Subcellular Location Cytoplasm, cytosol. Endoplasmic reticulum- Golgi intermediate compartment. Early endosome membrane. Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, phagosome membrane . Golgi apparatus.
Subunit Interacts with APPL1, FAM109A and FAM109B. Interacts with several Rab GTPases, at least RAB1A, RAB2A, RAB5A, RAB6A, RAB8A, RAB9A and RAB33B; these interactions may play a dual role in targeting INPP5B to the specific membranes and stimulating the phosphatase activity. {ECO
Tissue Specificity Platelets.

Identical and Related Proteins

Unique RefSeq proteins for LMP000303 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
113722125 RefSeq NP_005531 913 type II inositol 1,4,5-trisphosphate 5-phosphatase isoform 1 precursor
661903016 RefSeq NP_001284363 749 type II inositol 1,4,5-trisphosphate 5-phosphatase isoform 2 precursor

Identical Sequences to LMP000303 proteins

Reference Database Accession Length Protein Name

Related Sequences to LMP000303 proteins

Reference Database Accession Length Protein Name
GI:661903016 DBBJ BAG51126.1 749 unnamed protein product [Homo sapiens]
GI:661903016 EMBL CAE91541.1 749 unnamed protein product [Homo sapiens]
GI:113722125 GenBank AAA79207.1 942 inositol polyphosphate 5-phosphatase, partial [Homo sapiens]
GI:113722125 GenBank AAE27691.1 942 Sequence 4 from patent US 5955338
GI:661903016 GenBank EAX07307.1 749 inositol polyphosphate-5-phosphatase, 75kDa, isoform CRA_a [Homo sapiens]
GI:113722125 GenBank EAX07309.1 913 inositol polyphosphate-5-phosphatase, 75kDa, isoform CRA_c [Homo sapiens]
GI:113722125 GenBank EAX07310.1 913 inositol polyphosphate-5-phosphatase, 75kDa, isoform CRA_d [Homo sapiens]
GI:113722125 GenBank AFL28618.1 1017 Sequence 178 from patent US 8168602
GI:661903016 RefSeq XP_004025543.1 993 PREDICTED: type II inositol 1,4,5-trisphosphate 5-phosphatase [Gorilla gorilla gorilla]
GI:113722125 RefSeq XP_005270890.1 911 PREDICTED: type II inositol 1,4,5-trisphosphate 5-phosphatase isoform X1 [Homo sapiens]
GI:661903016 RefSeq XP_009452351.1 993 PREDICTED: type II inositol 1,4,5-trisphosphate 5-phosphatase isoform X1 [Pan troglodytes]
GI:661903016 SwissProt P32019.4 993 RecName: Full=Type II inositol 1,4,5-trisphosphate 5-phosphatase; AltName: Full=75 kDa inositol polyphosphate-5-phosphatase; AltName: Full=Phosphoinositide 5-phosphatase; Short=5PTase; Flags: Precursor [Homo sapiens]