Gene/Proteome Database (LMPD)

LMPD ID
LMP000308
Gene ID
5582
Species
Homo sapiens (Human)
Gene Name
protein kinase C, gamma
Gene Symbol
PRKCG
Synonyms
PKC-gamma; PKCC; PKCG; SCA14
Alternate Names
protein kinase C gamma type
Chromosome
19
Map Location
19q13.4
EC Number
2.7.11.13
Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for PRKCG

Proteins

protein kinase C gamma type
Refseq ID NP_002730
Protein GI 13384594
UniProt ID P05129
mRNA ID NM_002739
Length 697
RefSeq Status REVIEWED
MAGLGPGVGDSEGGPRPLFCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLCGVDHTERRGRLQLEIRAPTADEIHVTVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTRTVKATLNPVWNETFVFNLKPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSELLKAPVDGWYKLLNQEEGEYYNVPVADADNCSLLQKFEACNYPLELYERVRMGPSSSPIPSPSPSPTDPKRCFFGASPGRLHISDFSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGTTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVM

Gene Information

Entrez Gene ID
5582
Gene Name
protein kinase C, gamma
Gene Symbol
PRKCG
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005911 IEA:Ensembl C cell-cell junction
GO:0005829 ISS:UniProtKB C cytosol
GO:0030425 ISS:UniProtKB C dendrite
GO:0005634 IEA:Ensembl C nucleus
GO:0048471 ISS:UniProtKB C perinuclear region of cytoplasm
GO:0005886 ISS:UniProtKB C plasma membrane
GO:0097060 IEA:Ensembl C synaptic membrane
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0004698 IEA:Ensembl F calcium-dependent protein kinase C activity
GO:0004672 IDA:HGNC F protein kinase activity
GO:0004697 TAS:ProtInc F protein kinase C activity
GO:0004712 IDA:MGI F protein serine/threonine/tyrosine kinase activity
GO:0008270 IEA:InterPro F zinc ion binding
GO:0007202 TAS:Reactome P activation of phospholipase C activity
GO:0007596 TAS:Reactome P blood coagulation
GO:0008219 IEA:UniProtKB-KW P cell death
GO:0007635 IEA:Ensembl P chemosensory behavior
GO:0007173 TAS:Reactome P epidermal growth factor receptor signaling pathway
GO:0008543 TAS:Reactome P fibroblast growth factor receptor signaling pathway
GO:0045087 TAS:Reactome P innate immune response
GO:0060384 IEA:Ensembl P innervation
GO:0035556 IEA:InterPro P intracellular signal transduction
GO:0007611 IEA:Ensembl P learning or memory
GO:0043524 ISS:UniProtKB P negative regulation of neuron apoptotic process
GO:0042177 IDA:HGNC P negative regulation of protein catabolic process
GO:0031397 IDA:HGNC P negative regulation of protein ubiquitination
GO:0048011 TAS:Reactome P neurotrophin TRK receptor signaling pathway
GO:0016310 IDA:HGNC P phosphorylation
GO:0030168 TAS:Reactome P platelet activation
GO:0032425 IDA:HGNC P positive regulation of mismatch repair
GO:0046777 IEA:Ensembl P protein autophosphorylation
GO:0006468 TAS:ProtInc P protein phosphorylation
GO:0043278 ISS:UniProtKB P response to morphine
GO:0048265 ISS:UniProtKB P response to pain
GO:0007165 TAS:Reactome P signal transduction
GO:0007268 TAS:Reactome P synaptic transmission

KEGG Pathway Links

KEGG Pathway ID Description
hsa04750 Inflammatory mediator regulation of TRP channels
hsa04921 Oxytocin signaling pathway
hsa04015 Rap1 signaling pathway
hsa04919 Thyroid hormone signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR000961 AGC-kinase, C-terminal
IPR000008 C2 domain
IPR020454 Diacylglycerol/phorbol-ester binding
IPR017441 Protein kinase, ATP binding site
IPR014375 Protein kinase C, alpha/beta/gamma types
IPR002219 Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
IPR017892 Protein kinase, C-terminal
IPR000719 Protein kinase domain
IPR011009 Protein kinase-like domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR008271 Serine/threonine-protein kinase, active site

UniProt Annotations

Entry Information

Gene Name
protein kinase C, gamma
Protein Entry
KPCG_HUMAN
UniProt ID
P05129
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P05129-1; Sequence=Displayed; Name=2; IsoId=P05129-2; Sequence=VSP_056467, VSP_056468, VSP_056469; Note=No experimental confirmation available.;
Catalytic Activity ATP + a protein = ADP + a phosphoprotein.
Cofactor Name=Ca(2+); Xref=ChEBI
Disease Spinocerebellar ataxia 14 (SCA14) [MIM
Enzyme Regulation Classical (or conventional) PKCs (PRKCA, PRKCB and PRKCG) are activated by calcium and diacylglycerol (DAG) in the presence of phosphatidylserine. Three specific sites; Thr-514 (activation loop of the kinase domain), Thr-655 (turn motif) and Thr-674 (hydrophobic region), need to be phosphorylated for its full activation.
Function Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress (By similarity). Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage.
Ptm Autophosphorylation on Thr-674 appears to regulate motor functions of junctophilins, JPH3 and JPH4.
Ptm Ubiquitinated.
Similarity Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Similarity Contains 1 AGC-kinase C-terminal domain.
Similarity Contains 1 C2 domain. {ECO
Similarity Contains 1 protein kinase domain.
Similarity Contains 2 phorbol-ester/DAG-type zinc fingers.
Subcellular Location Cytoplasm . Cytoplasm, perinuclear region {ECO
Subunit Interacts with GRIA4 (By similarity). Interacts with CDCP1. Interacts with TP53INP1 and p53/TP53. {ECO
Tissue Specificity Expressed in Purkinje cells of the cerebellar cortex.
Web Resource Name=Mutations of the PRKCG gene; Note=Retina International's Scientific Newsletter; URL="http://www.retina-international.org/files/sci-news/prkcgmut.htm";

Identical and Related Proteins

Unique RefSeq proteins for LMP000308 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
13384594 RefSeq NP_002730 697 protein kinase C gamma type

Identical Sequences to LMP000308 proteins

Reference Database Accession Length Protein Name
GI:13384594 GenBank ADE98202.1 697 Sequence 2 from patent US 7655401
GI:13384594 GenBank ADM03572.1 697 Sequence 167 from patent US 7727958
GI:13384594 GenBank ADS40180.1 697 Sequence 123 from patent US 7792665
GI:13384594 GenBank AGV86230.1 697 Sequence 167 from patent US 8524673
GI:13384594 GenBank AHE00794.1 697 Sequence 53751 from patent US 8586006
GI:13384594 GenBank AIC62576.1 697 PRKCG, partial [synthetic construct]

Related Sequences to LMP000308 proteins

Reference Database Accession Length Protein Name
GI:13384594 DBBJ BAG35228.1 697 unnamed protein product [Homo sapiens]
GI:13384594 DBBJ BAH12352.1 710 unnamed protein product [Homo sapiens]
GI:13384594 EMBL CBY18310.1 673 unnamed protein product [Oikopleura dioica]
GI:13384594 GenBank ACM81848.1 725 Sequence 7346 from patent US 6812339
GI:13384594 RefSeq XP_005414928.1 697 PREDICTED: protein kinase C gamma type isoform X1 [Chinchilla lanigera]
GI:13384594 RefSeq NP_001270323.1 697 protein kinase C gamma type [Macaca fascicularis]