Gene/Proteome Database (LMPD)

LMPD ID
LMP000385
Gene ID
335
Species
Homo sapiens (Human)
Gene Name
apolipoprotein A-I
Gene Symbol
APOA1
Synonyms
-
Alternate Names
apolipoprotein A-I; apo-AI
Chromosome
11
Map Location
11q23-q24
Summary
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for APOA1

Proteins

apolipoprotein A-I preproprotein
Refseq ID NP_000030
Protein GI 4557321
UniProt ID P02647
mRNA ID NM_000039
Length 267
RefSeq Status REVIEWED
MKAAVLTLAVLFLTGSQARHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ
sig_peptide: 1..18 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 1834 peptide sequence: MKAAVLTLAVLFLTGSQA mat_peptide: 19..267 product: Proapolipoprotein A-I experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P02647.1) calculated_mol_wt: 28962 peptide sequence: RHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ mat_peptide: 25..267 product: apolipoprotein A-I calculated_mol_wt: 28079 peptide sequence: DEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ mat_peptide: 25..266 product: Truncated apolipoprotein A-I experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P02647.1) calculated_mol_wt: 27951 peptide sequence: DEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNT

Gene Information

Entrez Gene ID
335
Gene Name
apolipoprotein A-I
Gene Symbol
APOA1
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0072562 IDA:UniProt C blood microparticle
GO:0031410 IDA:BHF-UCL C cytoplasmic vesicle
GO:0005829 TAS:Reactome C cytosol
GO:0005769 TAS:Reactome C early endosome
GO:0030139 IDA:BHF-UCL C endocytic vesicle
GO:0071682 TAS:Reactome C endocytic vesicle lumen
GO:0005788 TAS:Reactome C endoplasmic reticulum lumen
GO:0005576 TAS:Reactome C extracellular region
GO:0005615 IDA:BHF-UCL C extracellular space
GO:0070062 IDA:UniProtKB C extracellular vesicular exosome
GO:0034364 IDA:BHF-UCL C high-density lipoprotein particle
GO:0005634 IEA:Ensembl C nucleus
GO:0005886 TAS:Reactome C plasma membrane
GO:0034774 TAS:Reactome C secretory granule lumen
GO:0034366 IDA:BHF-UCL C spherical high-density lipoprotein particle
GO:0034361 IDA:BHF-UCL C very-low-density lipoprotein particle
GO:0031982 IDA:UniProtKB C vesicle
GO:0034191 IPI:BHF-UCL F apolipoprotein A-I receptor binding
GO:0034190 IPI:BHF-UCL F apolipoprotein receptor binding
GO:0001540 IDA:BHF-UCL F beta-amyloid binding
GO:0045499 IDA:UniProtKB F chemorepellent activity
GO:0015485 IDA:BHF-UCL F cholesterol binding
GO:0017127 IMP:BHF-UCL F cholesterol transporter activity
GO:0019899 IPI:BHF-UCL F enzyme binding
GO:0008035 IEA:Ensembl F high-density lipoprotein particle binding
GO:0070653 IPI:BHF-UCL F high-density lipoprotein particle receptor binding
GO:0042802 IPI:IntAct F identical protein binding
GO:0055102 IEA:Ensembl F lipase inhibitor activity
GO:0060228 IDA:BHF-UCL F phosphatidylcholine-sterol O-acyltransferase activator activity
GO:0005543 IDA:BHF-UCL F phospholipid binding
GO:0005548 IEA:Ensembl F phospholipid transporter activity
GO:0030325 IEA:Ensembl P adrenal gland development
GO:0007596 TAS:Reactome P blood coagulation
GO:0043534 IEA:Ensembl P blood vessel endothelial cell migration
GO:0044255 TAS:Reactome P cellular lipid metabolic process
GO:0006695 IEA:Ensembl P cholesterol biosynthetic process
GO:0033344 IDA:BHF-UCL P cholesterol efflux
GO:0042632 IDA:BHF-UCL P cholesterol homeostasis
GO:0070508 IMP:BHF-UCL P cholesterol import
GO:0008203 IMP:BHF-UCL P cholesterol metabolic process
GO:0030301 IDA:MGI P cholesterol transport
GO:0001935 IEA:Ensembl P endothelial cell proliferation
GO:0008211 IEA:Ensembl P glucocorticoid metabolic process
GO:0007186 IDA:BHF-UCL P G-protein coupled receptor signaling pathway
GO:0034380 IDA:BHF-UCL P high-density lipoprotein particle assembly
GO:0034384 IC:BHF-UCL P high-density lipoprotein particle clearance
GO:0034375 IC:BHF-UCL P high-density lipoprotein particle remodeling
GO:0007229 IDA:UniProtKB P integrin-mediated signaling pathway
GO:0019915 IEA:Ensembl P lipid storage
GO:0042158 IEA:Ensembl P lipoprotein biosynthetic process
GO:0042157 TAS:Reactome P lipoprotein metabolic process
GO:0050919 IDA:UniProtKB P negative chemotaxis
GO:0060354 IDA:BHF-UCL P negative regulation of cell adhesion molecule production
GO:0002740 IDA:BHF-UCL P negative regulation of cytokine secretion involved in immune response
GO:0034115 IDA:BHF-UCL P negative regulation of heterotypic cell-cell adhesion
GO:0050728 IDA:BHF-UCL P negative regulation of inflammatory response
GO:0050713 IDA:BHF-UCL P negative regulation of interleukin-1 beta secretion
GO:0060192 IEA:Ensembl P negative regulation of lipase activity
GO:0060761 IDA:BHF-UCL P negative regulation of response to cytokine stimulus
GO:0010804 IDA:BHF-UCL P negative regulation of tumor necrosis factor-mediated signaling pathway
GO:0010903 IDA:BHF-UCL P negative regulation of very-low-density lipoprotein particle remodeling
GO:0031100 IEA:Ensembl P organ regeneration
GO:0018206 IDA:UniProtKB P peptidyl-methionine modification
GO:0014012 IEA:Ensembl P peripheral nervous system axon regeneration
GO:0006656 IDA:BHF-UCL P phosphatidylcholine biosynthetic process
GO:0033700 IDA:BHF-UCL P phospholipid efflux
GO:0055091 IDA:BHF-UCL P phospholipid homeostasis
GO:0007603 TAS:Reactome P phototransduction, visible light
GO:0030168 TAS:Reactome P platelet activation
GO:0002576 TAS:Reactome P platelet degranulation
GO:0010873 IDA:BHF-UCL P positive regulation of cholesterol esterification
GO:0051345 IDA:BHF-UCL P positive regulation of hydrolase activity
GO:0035025 IDA:UniProtKB P positive regulation of Rho protein signal transduction
GO:0051496 IDA:UniProtKB P positive regulation of stress fiber assembly
GO:1900026 IDA:UniProtKB P positive regulation of substrate adhesion-dependent cell spreading
GO:0051347 IEA:Ensembl P positive regulation of transferase activity
GO:0018158 IDA:UniProtKB P protein oxidation
GO:0050821 IDA:BHF-UCL P protein stabilization
GO:0032489 IDA:BHF-UCL P regulation of Cdc42 protein signal transduction
GO:0030300 IEA:Ensembl P regulation of intestinal cholesterol absorption
GO:0001932 IEA:Ensembl P regulation of protein phosphorylation
GO:0042493 IEA:Ensembl P response to drug
GO:0043627 IEA:Ensembl P response to estrogen
GO:0007584 IEA:Ensembl P response to nutrient
GO:0001523 TAS:Reactome P retinoid metabolic process
GO:0043691 IMP:BHF-UCL P reverse cholesterol transport
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0055085 TAS:Reactome P transmembrane transport
GO:0070328 IDA:BHF-UCL P triglyceride homeostasis

KEGG Pathway Links

KEGG Pathway ID Description
hsa05143 African trypanosomiasis
hsa04975 Fat digestion and absorption
hsa04977 Vitamin digestion and absorption

Domain Information

InterPro Annotations

Accession Description
IPR000074 Apolipoprotein A/E

UniProt Annotations

Entry Information

Gene Name
apolipoprotein A-I
Protein Entry
APOA1_HUMAN
UniProt ID
P02647
Species
Human

Comments

Comment Type Description
Disease Amyloidosis 8 (AMYL8) [MIM
Disease High density lipoprotein deficiency 1 (HDLD1) [MIM
Disease High density lipoprotein deficiency 2 (HDLD2) [MIM
Disease Note=APOA1 mutations may be involved in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III (PubMed:3142462 and PubMed:2123470). The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis.
Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Interaction Self; NbExp=12; IntAct=EBI-701692, EBI-701692; O95477:ABCA1; NbExp=4; IntAct=EBI-701692, EBI-784112; P05067:APP; NbExp=5; IntAct=EBI-701692, EBI-77613; P02671:FGA; NbExp=2; IntAct=EBI-701692, EBI-348571; P00738:HP; NbExp=3; IntAct=EBI-701692, EBI-1220767;
Mass Spectrometry Mass=28081; Method=Electrospray; Range=25-267; Note=Without methionine sulfoxide.; Evidence= ;
Mass Spectrometry Mass=28095; Method=Electrospray; Range=25-267; Note=With 1 methionine sulfoxide, oxidation at Met-136.; Evidence= ;
Mass Spectrometry Mass=28098; Method=Electrospray; Range=25-267; Note=With 1 methionine sulfoxide, oxidation at Met-110.; Evidence= ;
Mass Spectrometry Mass=28114; Method=Electrospray; Range=25-267; Note=With 2 methionine sulfoxides, oxidation at Met-110 and Met- 136.; Evidence= ;
Polymorphism Genetic variations in APOA1 can result in APOA1 deficiency and are associated with low levels of HDL cholesterol [MIM
Ptm Glycosylated.
Ptm Palmitoylated.
Ptm Phosphorylation sites are present in the extracellular medium.
Similarity Belongs to the apolipoprotein A1/A4/E family.
Subcellular Location Secreted.
Subunit Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. {ECO
Tissue Specificity Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
Web Resource Name=SHMPD; Note=The Singapore human mutation and polymorphism database; URL="http://shmpd.bii.a-star.edu.sg/gene.php?genestart=A&genename=APOA1";

Identical and Related Proteins

Unique RefSeq proteins for LMP000385 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
4557321 RefSeq NP_000030 267 apolipoprotein A-I preproprotein

Identical Sequences to LMP000385 proteins

Reference Database Accession Length Protein Name
GI:4557321 GenBank AGP16938.1 267 Sequence 1 from patent US 8460889
GI:4557321 GenBank AGX47856.1 267 Sequence 2 from patent US 8541236
GI:4557321 GenBank AHE01107.1 267 Sequence 56023 from patent US 8586006
GI:4557321 GenBank AIC48276.1 267 APOA1, partial [synthetic construct]
GI:4557321 RefSeq XP_005271596.1 267 PREDICTED: apolipoprotein A-I isoform X1 [Homo sapiens]
GI:4557321 RefSeq XP_005271597.1 267 PREDICTED: apolipoprotein A-I isoform X2 [Homo sapiens]

Related Sequences to LMP000385 proteins

Reference Database Accession Length Protein Name
GI:4557321 GenBank AAA35545.1 267 proapo-A-I protein [Homo sapiens]
GI:4557321 GenBank AAV38908.1 268 apolipoprotein A-I, partial [synthetic construct]
GI:4557321 GenBank AAV38929.1 268 apolipoprotein A-I, partial [synthetic construct]
GI:4557321 GenBank AAX42892.1 268 apolipoprotein A-I, partial [synthetic construct]
GI:4557321 GenBank AAX42893.1 268 apolipoprotein A-I, partial [synthetic construct]
GI:4557321 GenBank AGB62516.1 296 Sequence 77 from patent US 8288091