Gene/Proteome Database (LMPD)

LMPD ID
LMP000424
Gene ID
69923
Species
Mus musculus (Mouse)
Gene Name
acylglycerol kinase
Gene Symbol
Agk
Synonyms
2610037M15Rik; 6720408I04Rik; AI465370; Mulk
Alternate Names
acylglycerol kinase, mitochondrial; multi-substrate lipid kinase; multiple substrate lipid kinase
Chromosome
6
Map Location
6 B1|6
EC Number
2.7.1.107

Proteins

acylglycerol kinase, mitochondrial precursor
Refseq ID NP_076027
Protein GI 37537518
UniProt ID Q9ESW4
mRNA ID NM_023538
Length 421
RefSeq Status VALIDATED
MTAFFKTLRNHWKKTTAGLCLLTWGGHWLYGKHCDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFLNPAACKGKARTLFEKNAAPILHLSGMDVTVVKTDYEGQAKKLLELMESTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGQTSSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVYAMTGLRWGSFRDAGVKVSKYWYLGPLKTKAAHFFSTLQEWPQTHQASISYTGPRERPPIEPEETPPRPSLYRRILRRLASFWAQPQDASSREVSPEVWKDVQLSTIELSITTRNTQLDLMSKEDFMNICIEPDTVSKGDFIIIGSKKVRDPGLRAAGTECLQASHCTLVLPEGTEGSFSIDSEEYEAMPVEVKLLPRKLRFFCDPRKREQMLPSTSQ
transit_peptide: 1..31 inference: non-experimental evidence, no additional details recorded note: Mitochondrion (Potential); propagated from UniProtKB/Swiss-Prot (Q9ESW4.1) calculated_mol_wt: 3639 peptide sequence: MTAFFKTLRNHWKKTTAGLCLLTWGGHWLYG mat_peptide: 32..421 product: Acylglycerol kinase, mitochondrial experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q9ESW4.1) calculated_mol_wt: 43355 peptide sequence: KHCDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFLNPAACKGKARTLFEKNAAPILHLSGMDVTVVKTDYEGQAKKLLELMESTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGQTSSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVYAMTGLRWGSFRDAGVKVSKYWYLGPLKTKAAHFFSTLQEWPQTHQASISYTGPRERPPIEPEETPPRPSLYRRILRRLASFWAQPQDASSREVSPEVWKDVQLSTIELSITTRNTQLDLMSKEDFMNICIEPDTVSKGDFIIIGSKKVRDPGLRAAGTECLQASHCTLVLPEGTEGSFSIDSEEYEAMPVEVKLLPRKLRFFCDPRKREQMLPSTSQ

Gene Information

Entrez Gene ID
69923
Gene Name
acylglycerol kinase
Gene Symbol
Agk
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016020 IDA:MGI C membrane
GO:0005739 IDA:MGI C mitochondrion
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0003951 IEA:InterPro F NAD+ kinase activity
GO:0047620 IEA:UniProtKB-EC F acylglycerol kinase activity
GO:0001729 IDA:MGI F ceramide kinase activity
GO:0004143 IEA:UniProtKB-EC F diacylglycerol kinase activity
GO:0001727 IDA:MGI F lipid kinase activity
GO:0046513 IDA:MGI P ceramide biosynthetic process
GO:0046486 IEA:UniProtKB-UniPathway P glycerolipid metabolic process
GO:0046834 IDA:MGI P lipid phosphorylation
GO:0007205 IEA:InterPro P protein kinase C-activating G-protein coupled receptor signaling pathway

KEGG Pathway Links

KEGG Pathway ID Description
ko00561 Glycerolipid metabolism
mmu00561 Glycerolipid metabolism
mmu01100 Metabolic pathways

Domain Information

InterPro Annotations

Accession Description
IPR016064 ATP-NAD kinase-like domain
IPR001206 Diacylglycerol kinase, catalytic domain

UniProt Annotations

Entry Information

Gene Name
acylglycerol kinase
Protein Entry
AGK_MOUSE
UniProt ID
Q9ESW4
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9ESW4-1; Sequence=Displayed; Name=2; IsoId=Q9ESW4-2; Sequence=VSP_020927; Note=No experimental confirmation available.;
Biophysicochemical Properties Kinetic parameters: KM=45 uM for diacylglycerol {ECO:0000269|PubMed:15252046}; Vmax=159 nmol/min/mg enzyme with dioeoylglycerol as substrate {ECO:0000269|PubMed:15252046};
Catalytic Activity ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2- diacyl-sn-glycerol 3-phosphate.
Catalytic Activity ATP + acylglycerol = ADP + acyl-sn-glycerol 3- phosphate.
Caution Was originally (PubMed:15252046) thought to have ceramide kinase activity. Such activity is however unlikely in vivo. {ECO:0000305|PubMed:15252046}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
Function Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth. {ECO:0000269|PubMed:15252046}.
Pathway Lipid metabolism; glycerolipid metabolism.
Similarity Contains 1 DAGKc domain. {ECO:0000255|PROSITE- ProRule:PRU00783}.
Subcellular Location Mitochondrion membrane {ECO:0000250}.
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:15252046}.

Identical and Related Proteins

Unique RefSeq proteins for LMP000424 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
37537518 RefSeq NP_076027 421 acylglycerol kinase, mitochondrial precursor

Identical Sequences to LMP000424 proteins

Reference Database Accession Length Protein Name
GI:37537518 DBBJ BAC36260.1 421 unnamed protein product [Mus musculus]
GI:37537518 GenBank AAH19145.1 421 Acylglycerol kinase [Mus musculus]
GI:37537518 GenBank AAV38106.1 421 multi-substrate lipid kinase [Mus musculus]
GI:37537518 GenBank AAH93525.1 421 Acylglycerol kinase [Mus musculus]
GI:37537518 GenBank EDL13588.1 421 mCG121383, isoform CRA_a [Mus musculus]
GI:37537518 SwissProt Q9ESW4.1 421 RecName: Full=Acylglycerol kinase, mitochondrial; AltName: Full=Multiple substrate lipid kinase; Short=MuLK; Short=Multi-substrate lipid kinase; Flags: Precursor [Mus musculus]

Related Sequences to LMP000424 proteins

Reference Database Accession Length Protein Name
GI:37537518 GenBank EDM15405.1 421 similar to putative lipid kinase (predicted), isoform CRA_c [Rattus norvegicus]
GI:37537518 RefSeq NP_001120969.1 421 acylglycerol kinase, mitochondrial [Rattus norvegicus]
GI:37537518 RefSeq XP_005085817.1 421 PREDICTED: acylglycerol kinase, mitochondrial [Mesocricetus auratus]
GI:37537518 RefSeq XP_006236425.1 490 PREDICTED: acylglycerol kinase, mitochondrial isoform X2 [Rattus norvegicus]
GI:37537518 RefSeq XP_006997144.1 421 PREDICTED: acylglycerol kinase, mitochondrial [Peromyscus maniculatus bairdii]
GI:37537518 RefSeq XP_008761043.1 421 PREDICTED: acylglycerol kinase, mitochondrial isoform X1 [Rattus norvegicus]