Gene/Proteome Database (LMPD)

LMPD ID
LMP000430
Gene ID
1544
Species
Homo sapiens (Human)
Gene Name
cytochrome P450, family 1, subfamily A, polypeptide 2
Gene Symbol
CYP1A2
Synonyms
CP12; P3-450; P450(PA)
Alternate Names
cytochrome P450 1A2; CYPIA2; P450 form 4; cytochrome P450 4; cytochrome P(3)450; cytochrome P450-P3; dioxin-inducible P3-450; microsomal monooxygenase; xenobiotic monooxygenase; aryl hydrocarbon hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2
Chromosome
15
Map Location
15q24.1
EC Number
1.14.14.1
Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for CYP1A2

Proteins

cytochrome P450 1A2
Refseq ID NP_000752
Protein GI 73915100
UniProt ID P05177
mRNA ID NM_000761
Length 516
RefSeq Status REVIEWED
MALSQSVPFSATELLLASAIFCLVFWVLKGLRPRVPKGLKSPPEPWGWPLLGHVLTLGKNPHLALSRMSQRYGDVLQIRIGSTPVLVLSRLDTIRQALVRQGDDFKGRPDLYTSTLITDGQSLTFSTDSGPVWAARRRLAQNALNTFSIASDPASSSSCYLEEHVSKEAKALISRLQELMAGPGHFDPYNQVVVSVANVIGAMCFGQHFPESSDEMLSLVKNTHEFVETASSGNPLDFFPILRYLPNPALQRFKAFNQRFLWFLQKTVQEHYQDFDKNSVRDITGALFKHSKKGPRASGNLIPQEKIVNLVNDIFGAGFDTVTTAISWSLMYLVTKPEIQRKIQKELDTVIGRERRPRLSDRPQLPYLEAFILETFRHSSFLPFTIPHSTTRDTTLNGFYIPKKCCVFVNQWQVNHDPELWEDPSEFRPERFLTADGTAINKPLSEKMMLFGMGKRRCIGEVLAKWEIFLFLAILLQQLEFSVPPGVKVDLTPIYGLTMKHARCEHVQARLRFSIN

Gene Information

Entrez Gene ID
1544
Gene Name
cytochrome P450, family 1, subfamily A, polypeptide 2
Gene Symbol
CYP1A2
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005789 TAS:Reactome C endoplasmic reticulum membrane
GO:0043231 IDA:UniProtKB C intracellular membrane-bounded organelle
GO:0070330 IEA:UniProtKB-EC F aromatase activity
GO:0034875 IDA:BHF-UCL F caffeine oxidase activity
GO:0032451 IDA:UniProtKB F demethylase activity
GO:0009055 TAS:UniProtKB F electron carrier activity
GO:0019899 IPI:BHF-UCL F enzyme binding
GO:0020037 IDA:UniProtKB F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0004497 IDA:BHF-UCL F monooxygenase activity
GO:0016491 IDA:BHF-UCL F oxidoreductase activity
GO:0016712 IMP:UniProtKB F oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
GO:0009820 IDA:BHF-UCL P alkaloid metabolic process
GO:0019369 TAS:Reactome P arachidonic acid metabolic process
GO:0045333 IEA:Ensembl P cellular respiration
GO:0071276 IEA:Ensembl P cellular response to cadmium ion
GO:0018894 IEA:Ensembl P dibenzo-p-dioxin metabolic process
GO:0042737 IMP:BHF-UCL P drug catabolic process
GO:0017144 IDA:BHF-UCL P drug metabolic process
GO:0019373 TAS:Reactome P epoxygenase P450 pathway
GO:0042738 IDA:BHF-UCL P exogenous drug catabolic process
GO:0046483 IDA:BHF-UCL P heterocycle metabolic process
GO:0050665 IEA:Ensembl P hydrogen peroxide biosynthetic process
GO:0030324 IEA:Ensembl P lung development
GO:0032259 TAS:Reactome P methylation
GO:0032787 IDA:BHF-UCL P monocarboxylic acid metabolic process
GO:0016098 IDA:BHF-UCL P monoterpenoid metabolic process
GO:0097267 TAS:Reactome P omega-hydroxylase P450 pathway
GO:0055114 IDA:BHF-UCL P oxidation-reduction process
GO:0071615 IDA:BHF-UCL P oxidative deethylation
GO:0070989 IDA:BHF-UCL P oxidative demethylation
GO:0006778 IEA:Ensembl P porphyrin-containing compound metabolic process
GO:0009791 IEA:Ensembl P post-embryonic development
GO:0010468 IEA:Ensembl P regulation of gene expression
GO:0032355 IEA:Ensembl P response to estradiol
GO:0032496 IEA:Ensembl P response to lipopolysaccharide
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0006706 IMP:BHF-UCL P steroid catabolic process
GO:0009403 IDA:BHF-UCL P toxin biosynthetic process
GO:0006805 TAS:Reactome P xenobiotic metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
hsa05204 Chemical carcinogenesis
hsa00140 Steroid hormone biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR001128 Cytochrome P450
IPR002401 Cytochrome P450, E-class, group I
IPR008066 Cytochrome P450, E-class, group I, CYP1
IPR017972 Cytochrome P450, conserved site

UniProt Annotations

Entry Information

Gene Name
cytochrome P450, family 1, subfamily A, polypeptide 2
Protein Entry
CP1A2_HUMAN
UniProt ID
P05177
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P05177-1; Sequence=Displayed; Name=2; IsoId=P05177-2; Sequence=VSP_017123;
Biophysicochemical Properties Kinetic parameters: KM=4 uM for 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole ; KM=21 uM for 2-amino-3-methylimidazo[4,5-f]quinoline ; KM=26 uM for 2-amino-2,4-dimethylimidazo[4,5-f]quinoline ; KM=27 uM for 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline ; KM=71 uM for 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine ; KM=25 uM for phenacetin ;
Catalytic Activity RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.
Cofactor Name=heme; Xref=ChEBI
Function Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O- deethylation of phenacetin.
Induction By nicotine, omeprazole, phenobarbital, primidone and rifampicin.
Polymorphism The CYP1A2*1F allele which is quite common (40 to 50%) is due to a substitution of a base in the non-coding region of the CYP1A2 gene and has the effect of decreasing the enzyme inducibility. Individuals who are homozygous for the CYP1A2*1F allele are 'slow' caffeine metabolizers. Thus for these individual increased intake of caffeine seems to be associated with a concomitant increase in the risk of non-fatal myocardial infraction (MI).
Similarity Belongs to the cytochrome P450 family.
Subcellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue Specificity Liver.
Web Resource Name=Cytochrome P450 Allele Nomenclature Committee; Note=CYP1A2 alleles; URL="http://www.cypalleles.ki.se/cyp1a2.htm";
Web Resource Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/cyp1a2/";

Identical and Related Proteins

Unique RefSeq proteins for LMP000430 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
73915100 RefSeq NP_000752 516 cytochrome P450 1A2

Identical Sequences to LMP000430 proteins

Reference Database Accession Length Protein Name
GI:73915100 GenBank EAW99313.1 516 cytochrome P450, family 1, subfamily A, polypeptide 2 [Homo sapiens]
GI:73915100 GenBank ACM85271.1 516 Sequence 10769 from patent US 6812339
GI:73915100 GenBank AEP61331.1 516 Sequence 13 from patent US 8026085
GI:73915100 GenBank AFQ85864.1 516 Sequence 13 from patent US 8252559
GI:73915100 GenBank AHE01159.1 516 Sequence 56075 from patent US 8586006
GI:73915100 GenBank AIC48604.1 516 CYP1A2, partial [synthetic construct]

Related Sequences to LMP000430 proteins

Reference Database Accession Length Protein Name
GI:73915100 EMBL CAA77335.1 515 unnamed protein product [Homo sapiens]
GI:73915100 GenBank AAA35738.1 516 cytochrome P450 4 [Homo sapiens]
GI:73915100 GenBank AAF13599.1 516 cytochrome P450-1A2 [Homo sapiens]
GI:73915100 GenBank AAH67427.1 516 Cytochrome P450, family 1, subfamily A, polypeptide 2 [Homo sapiens]
GI:73915100 GenBank AAH67429.1 516 Cytochrome P450, family 1, subfamily A, polypeptide 2 [Homo sapiens]
GI:73915100 GenBank ACM80490.1 515 Sequence 5988 from patent US 6812339