Gene/Proteome Database (LMPD)

LMPD ID
LMP000442
Gene ID
53947
Species
Homo sapiens (Human)
Gene Name
alpha 1,4-galactosyltransferase
Gene Symbol
A4GALT
Synonyms
A14GALT; A4GALT1; Gb3S; P(k); P1; P1PK; PK
Alternate Names
lactosylceramide 4-alpha-galactosyltransferase; GB3 synthase; alpha4Gal-T1; CD77 synthase; P1/Pk synthase; P(k) antigen synthase; P blood group (P one antigen); globotriaosylceramide synthase; alpha-1,4-N-acetylglucosaminyltransferase; UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase
Chromosome
22
Map Location
22q13.2
EC Number
2.4.1.228
Summary
The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. The encoded protein, which is a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for A4GALT

Proteins

lactosylceramide 4-alpha-galactosyltransferase
Refseq ID NP_059132
Protein GI 8392830
UniProt ID Q9NPC4
mRNA ID NM_017436
Length 353
RefSeq Status REVIEWED
MSKPPDLLLRLLRGAPRQRVCTLFIIGFKFTFFVSIMIYWHVVGEPKEKGQLYNLPAEIPCPTLTPPTPPSHGPTPGNIFFLETSDRTNPNFLFMCSVESAARTHPESHVLVLMKGLPGGNASLPRHLGISLLSCFPNVQMLPLDLRELFRDTPLADWYAAVQGRWEPYLLPVLSDASRIALMWKFGGIYLDTDFIVLKNLRNLTNVLGTQSRYVLNGAFLAFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINPEELPRLLSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEAMKMYL

Gene Information

Entrez Gene ID
53947
Gene Name
alpha 1,4-galactosyltransferase
Gene Symbol
A4GALT
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0070062 IDA:UniProt C extracellular vesicular exosome
GO:0030173 NAS:UniProtKB C integral component of Golgi membrane
GO:0016020 IDA:MGI C membrane
GO:0008378 IDA:UniProtKB F galactosyltransferase activity
GO:0050512 IEA:UniProtKB-EC F lactosylceramide 4-alpha-galactosyltransferase activity
GO:0015643 IEA:Ensembl F toxic substance binding
GO:0001576 IEA:Ensembl P globoside biosynthetic process
GO:0006688 NAS:UniProtKB P glycosphingolipid biosynthetic process
GO:0007009 IDA:MGI P plasma membrane organization
GO:0006486 IEA:UniProtKB-UniPathway P protein glycosylation

KEGG Pathway Links

KEGG Pathway ID Description
hsa00603 Glycosphingolipid biosynthesis - globo series
ko00603 Glycosphingolipid biosynthesis - globo series
hsa01100 Metabolic pathways

Domain Information

InterPro Annotations

Accession Description
IPR007652 Alpha 1,4-glycosyltransferase domain
IPR007577 Glycosyltransferase, DXD sugar-binding motif
IPR029044 Nucleotide-diphospho-sugar transferases

UniProt Annotations

Entry Information

Gene Name
alpha 1,4-galactosyltransferase
Protein Entry
A4GAT_HUMAN
UniProt ID
Q9NPC4
Species
Human

Comments

Comment Type Description
Catalytic Activity UDP-alpha-D-galactose + beta-D-galactosyl- (1->4)-D-glucosyl-(1<->1)-ceramide = UDP + alpha-D-galactosyl- (1->4)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide.
Domain The conserved DXD motif is involved in enzyme activity.
Function Necessary for the biosynthesis of the Pk antigen of blood histogroup P. Catalyzes the transfer of galactose to lactosylceramide and galactosylceramide. Necessary for the synthesis of the receptor for bacterial verotoxins.
Pathway Protein modification; protein glycosylation.
Polymorphism Genetic variation in A4GALT is responsible for the P system blood group phenotypes [MIM
Similarity Belongs to the glycosyltransferase 32 family.
Subcellular Location Golgi apparatus membrane ; Single-pass type II membrane protein .
Tissue Specificity Ubiquitous. Highly expressed in kidney, heart, spleen, liver, testis and placenta.
Web Resource Name=Functional Glycomics Gateway - GTase; Note=Lactosylceramide 4-alpha-galactosyltransferase; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_428";
Web Resource Name=GGDB; Note=GlycoGene database; URL="http://jcggdb.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=A4GALT";
Web Resource Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/a4galt/";

Identical and Related Proteins

Unique RefSeq proteins for LMP000442 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
8392830 RefSeq NP_059132 353 lactosylceramide 4-alpha-galactosyltransferase

Identical Sequences to LMP000442 proteins

Reference Database Accession Length Protein Name
GI:8392830 GenBank AIC51588.1 353 A4GALT, partial [synthetic construct]
GI:8392830 RefSeq XP_005261704.1 353 PREDICTED: lactosylceramide 4-alpha-galactosyltransferase isoform X5 [Homo sapiens]
GI:8392830 RefSeq XP_005261705.1 353 PREDICTED: lactosylceramide 4-alpha-galactosyltransferase isoform X6 [Homo sapiens]
GI:8392830 RefSeq XP_006724328.1 353 PREDICTED: lactosylceramide 4-alpha-galactosyltransferase isoform X7 [Homo sapiens]
GI:8392830 RefSeq XP_006724329.1 353 PREDICTED: lactosylceramide 4-alpha-galactosyltransferase isoform X8 [Homo sapiens]
GI:8392830 RefSeq XP_006724330.1 353 PREDICTED: lactosylceramide 4-alpha-galactosyltransferase isoform X9 [Homo sapiens]

Related Sequences to LMP000442 proteins

Reference Database Accession Length Protein Name
GI:8392830 GenBank AFM54542.1 353 alpha 1,4-galactosyltransferase [Homo sapiens]
GI:8392830 GenBank JAA04077.1 353 alpha 1,4-galactosyltransferase [Pan troglodytes]
GI:8392830 GenBank JAA14771.1 353 alpha 1,4-galactosyltransferase [Pan troglodytes]
GI:8392830 GenBank JAA27167.1 353 alpha 1,4-galactosyltransferase [Pan troglodytes]
GI:8392830 GenBank JAA36307.1 353 alpha 1,4-galactosyltransferase [Pan troglodytes]
GI:8392830 GenBank JAA36308.1 353 alpha 1,4-galactosyltransferase [Pan troglodytes]