Gene/Proteome Database (LMPD)
LMPD ID
LMP000449
Gene ID
Species
Homo sapiens (Human)
Gene Name
cytochrome P450, family 2, subfamily B, polypeptide 6
Gene Symbol
Synonyms
CPB6; CYP2B; CYP2B7; CYP2B7P; CYPIIB6; EFVM; IIB1; P450
Alternate Names
cytochrome P450 2B6; cytochrome P450 IIB1; 1,4-cineole 2-exo-monooxygenase; cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6
Chromosome
19
Map Location
19q13.2
EC Number
1.14.13.-
Summary
This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| cytochrome P450 2B6 precursor | |
|---|---|
| Refseq ID | NP_000758 |
| Protein GI | 7949031 |
| UniProt ID | P20813 |
| mRNA ID | NM_000767 |
| Length | 491 |
| RefSeq Status | REVIEWED |
| MELSVLLFLALLTGLLLLLVQRHPNTHDRLPPGPRPLPLLGNLLQMDRRGLLKSFLRFREKYGDVFTVHLGPRPVVMLCGVEAIREALVDKAEAFSGRGKIAMVDPFFRGYGVIFANGNRWKVLRRFSVTTMRDFGMGKRSVEERIQEEAQCLIEELRKSKGALMDPTFLFQSITANIICSIVFGKRFHYQDQEFLKMLNLFYQTFSLISSVFGQLFELFSGFLKYFPGAHRQVYKNLQEINAYIGHSVEKHRETLDPSAPKDLIDTYLLHMEKEKSNAHSEFSHQNLNLNTLSLFFAGTETTSTTLRYGFLLMLKYPHVAERVYREIEQVIGPHRPPELHDRAKMPYTEAVIYEIQRFSDLLPMGVPHIVTQHTSFRGYIIPKDTEVFLILSTALHDPHYFEKPDAFNPDHFLDANGALKKTEAFIPFSLGKRICLGEGIARAELFLFFTTILQNFSMASPVAPEDIDLTPQECGVGKIPPTYQIRFLPR | |
| sig_peptide: 1..22 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2469 peptide sequence: MELSVLLFLALLTGLLLLLVQR | |
Gene Information
Entrez Gene ID
Gene Name
cytochrome P450, family 2, subfamily B, polypeptide 6
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005789 | TAS:Reactome | C | endoplasmic reticulum membrane |
| GO:0020037 | IDA:UniProtKB | F | heme binding |
| GO:0005506 | IEA:InterPro | F | iron ion binding |
| GO:0004497 | IDA:BHF-UCL | F | monooxygenase activity |
| GO:0016712 | IEA:InterPro | F | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen |
| GO:0042180 | IDA:BHF-UCL | P | cellular ketone metabolic process |
| GO:0017144 | IDA:BHF-UCL | P | drug metabolic process |
| GO:0042738 | IDA:BHF-UCL | P | exogenous drug catabolic process |
| GO:0055114 | IDA:BHF-UCL | P | oxidation-reduction process |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
| GO:0008202 | IMP:BHF-UCL | P | steroid metabolic process |
| GO:0006805 | IDA:UniProtKB | P | xenobiotic metabolic process |
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| PWY66-241 | bupropion degradation |
Domain Information
UniProt Annotations
Entry Information
Gene Name
cytochrome P450, family 2, subfamily B, polypeptide 6
Protein Entry
CP2B6_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P20813-1; Sequence=Displayed; Name=2; IsoId=P20813-2; Sequence=VSP_055571, VSP_055572; Note=No experimental confirmation available.; |
| Biophysicochemical Properties | Kinetic parameters: KM=360 uM for 1,4-cineole ; Vmax=3.4 nmol/min/nmol enzyme toward 2-exo-hydroxy-1,4-cineole ; |
| Catalytic Activity | 1,4-cineole + NADPH + O(2) = 2-exo-hydroxy- 1,4-cineole + NADP(+) + H(2)O. |
| Cofactor | Name=heme; Xref=ChEBI |
| Function | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase. {ECO |
| Induction | By phenobarbital. |
| Polymorphism | Genetic variations in CYP2B6 are responsible for poor metabolism of efavirenz and, therefore, susceptibility to efavirenz toxicity in the central nervous system [MIM |
| Ptm | Phosphorylation is accompanied by a decrease in enzyme activity. |
| Similarity | Belongs to the cytochrome P450 family. |
| Subcellular Location | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| Tissue Specificity | Expressed in liver, lung and heart right ventricle. |
| Web Resource | Name=Cytochrome P450 Allele Nomenclature Committee; Note=CYP2B6 alleles; URL="http://www.cypalleles.ki.se/cyp2b6.htm"; |
| Web Resource | Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/cyp2b6/"; |
Identical and Related Proteins
Unique RefSeq proteins for LMP000449 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 7949031 | RefSeq | NP_000758 | 491 | cytochrome P450 2B6 precursor |
Identical Sequences to LMP000449 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:7949031 | GenBank | AAA52144.1 | 491 | cytochrome P450-IIB [Homo sapiens] |
| GI:7949031 | GenBank | AAF32444.1 | 491 | CYP2B6 [Homo sapiens] |
| GI:7949031 | GenBank | ABB84469.1 | 491 | cytochrome P450, family 2, subfamily B, polypeptide 6 [Homo sapiens] |
| GI:7949031 | GenBank | EAW57015.1 | 491 | cytochrome P450, family 2, subfamily B, polypeptide 6 [Homo sapiens] |
| GI:7949031 | GenBank | AGU30785.1 | 491 | Sequence 91 from patent US 8506948 |
| GI:7949031 | GenBank | AHE01166.1 | 491 | Sequence 56082 from patent US 8586006 |
Related Sequences to LMP000449 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:7949031 | GenBank | AAF13602.1 | 491 | cytochrome P450-2B6 [Homo sapiens] |
| GI:7949031 | GenBank | AAH67430.1 | 491 | Cytochrome P450, family 2, subfamily B, polypeptide 6 [Homo sapiens] |
| GI:7949031 | GenBank | AAH67431.1 | 491 | Cytochrome P450, family 2, subfamily B, polypeptide 6 [Homo sapiens] |
| GI:7949031 | GenBank | AIC54255.1 | 491 | CYP2B6, partial [synthetic construct] |
| GI:7949031 | PDB | 3QOA | 476 | Chain A, Crystal Structure Of A Human Cytochrome P450 2b6 (Y226hK262R) IN Complex With The Inhibitor 4-Benzylpyridine. |
| GI:7949031 | RefSeq | XP_008963122.1 | 491 | PREDICTED: cytochrome P450 2B6 [Pan paniscus] |