Gene/Proteome Database (LMPD)
LMPD ID
LMP000541
Gene ID
Species
Homo sapiens (Human)
Gene Name
cytochrome P450, family 8, subfamily B, polypeptide 1
Gene Symbol
Synonyms
CP8B; CYP12
Alternate Names
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; CYPVIIIB1; cytochrome P450 8B1; sterol 12-alpha-hydroxylase; 7 alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; cytochrome P450, subfamily VIIIB (sterol 12-alpha-hydroxylase), polypeptide 1
Chromosome
3
Map Location
3p22.1
EC Number
1.14.13.95
Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase | |
|---|---|
| Refseq ID | NP_004382 |
| Protein GI | 166362724 |
| UniProt ID | Q9UNU6 |
| mRNA ID | NM_004391 |
| Length | 501 |
| RefSeq Status | REVIEWED |
| MVLWGPVLGALLVVIAGYLCLPGMLRQRRPWEPPLDKGTVPWLGHAMAFRKNMFEFLKRMRTKHGDVFTVQLGGQYFTFVMDPLSFGSILKDTQRKLDFGQYAKKLVLKVFGYRSVQGDHEMIHSASTKHLRGDGLKDLNETMLDSLSFVMLTSKGWSLDASCWHEDSLFRFCYYILFTAGYLSLFGYTKDKEQDLLQAGELFMEFRKFDLLFPRFVYSLLWPREWLEVGRLQRLFHKMLSVSHSQEKEGISNWLGNMLQFLREQGVPSAMQDKFNFMMLWASQGNTGPTSFWALLYLLKHPEAIRAVREEATQVLGEARLETKQSFAFKLGALQHTPVLDSVVEETLRLRAAPTLLRLVHEDYTLKMSSGQEYLFRHGDILALFPYLSVHMDPDIHPEPTVFKYDRFLNPNGSRKVDFFKTGKKIHHYTMPWGSGVSICPGRFFALSEVKLFILLMVTHFDLELVDPDTPLPHVDPQRWGFGTMQPSHDVRFRYRLHPTE | |
Gene Information
Entrez Gene ID
Gene Name
cytochrome P450, family 8, subfamily B, polypeptide 1
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IBA:RefGenome | C | endoplasmic reticulum |
| GO:0005789 | TAS:Reactome | C | endoplasmic reticulum membrane |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0033778 | IEA:UniProtKB-EC | F | 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity |
| GO:0020037 | IBA:RefGenome | F | heme binding |
| GO:0005506 | IEA:InterPro | F | iron ion binding |
| GO:0019825 | TAS:ProtInc | F | oxygen binding |
| GO:0008397 | IBA:RefGenome | F | sterol 12-alpha-hydroxylase activity |
| GO:0006699 | TAS:Reactome | P | bile acid biosynthetic process |
| GO:0008206 | TAS:Reactome | P | bile acid metabolic process |
| GO:0006629 | IBA:RefGenome | P | lipid metabolic process |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
| GO:0016125 | TAS:Reactome | P | sterol metabolic process |
| GO:0006805 | TAS:Reactome | P | xenobiotic metabolic process |
KEGG Pathway Links
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_150149 | Synthesis of Prostaglandins (PG) and Thromboxanes (TX) |
Domain Information
UniProt Annotations
Entry Information
Gene Name
cytochrome P450, family 8, subfamily B, polypeptide 1
Protein Entry
CP8B1_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | 7-alpha-hydroxycholest-4-en-3-one + NADPH + O(2) = 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + NADP(+) + H(2)O. |
| Cofactor | Name=heme; Xref=ChEBI |
| Function | Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids. |
| Similarity | Belongs to the cytochrome P450 family. |
| Subcellular Location | Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein. |
| Tissue Specificity | Liver. |
Identical and Related Proteins
Unique RefSeq proteins for LMP000541 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 166362724 | RefSeq | NP_004382 | 501 | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
Identical Sequences to LMP000541 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:166362724 | GenBank | EAW64682.1 | 501 | cytochrome P450, family 8, subfamily B, polypeptide 1 [Homo sapiens] |
| GI:166362724 | SwissProt | Q9UNU6.2 | 501 | RecName: Full=7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; AltName: Full=7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; AltName: Full=CYPVIIIB1; AltName: Full=Cytochrome P450 8B1; AltName: Full=Sterol 12-alpha-hydroxylase [Homo sapiens] |
Related Sequences to LMP000541 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:166362724 | DBBJ | BAG37730.1 | 501 | unnamed protein product [Homo sapiens] |
| GI:166362724 | GenBank | AAD19877.1 | 501 | sterol 12-alpha-hydroxylase CYP8B1 [Homo sapiens] |
| GI:166362724 | GenBank | AAH67434.1 | 501 | Cytochrome P450, family 8, subfamily B, polypeptide 1 [Homo sapiens] |
| GI:166362724 | GenBank | AAH67442.1 | 501 | Cytochrome P450, family 8, subfamily B, polypeptide 1 [Homo sapiens] |
| GI:166362724 | GenBank | ACM82623.1 | 563 | Sequence 8121 from patent US 6812339 |
| GI:166362724 | GenBank | AHE01175.1 | 501 | Sequence 56091 from patent US 8586006 |