Gene/Proteome Database (LMPD)
LMPD ID
LMP000658
Gene ID
Species
Homo sapiens (Human)
Gene Name
protein kinase, AMP-activated, gamma 3 non-catalytic subunit
Gene Symbol
Synonyms
AMPKG3
Alternate Names
5'-AMP-activated protein kinase subunit gamma-3;,AMPK gamma3; AMPK gamma-3 chain; AMPK subunit gamma-3', "5'-AMP-activated protein kinase, gamma-3 subunit
Chromosome
2
Map Location
2q35
Summary
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| 5'-AMP-activated protein kinase subunit gamma-3 | |
|---|---|
| Refseq ID | NP_059127 |
| Protein GI | 47132577 |
| UniProt ID | Q9UGI9 |
| mRNA ID | NM_017431 |
| Length | 489 |
| RefSeq Status | REVIEWED |
| MEPGLEHALRRTPSWSSLGGSEHQEMSFLEQENSSSWPSPAVTSSSERIRGKRRAKALRWTRQKSVEEGEPPGQGEGPRSRPAAESTGLEATFPKTTPLAQADPAGVGTPPTGWDCLPSDCTASAAGSSTDDVELATEFPATEAWECELEGLLEERPALCLSPQAPFPKLGWDDELRKPGAQIYMRFMQEHTCYDAMATSSKLVIFDTMLEIKKAFFALVANGVRAAPLWDSKKQSFVGMLTITDFILVLHRYYRSPLVQIYEIEQHKIETWREIYLQGCFKPLVSISPNDSLFEAVYTLIKNRIHRLPVLDPVSGNVLHILTHKRLLKFLHIFGSLLPRPSFLYRTIQDLGIGTFRDLAVVLETAPILTALDIFVDRRVSALPVVNECGQVVGLYSRFDVIHLAAQQTYNHLDMSVGEALRQRTLCLEGVLSCQPHESLGEVIDRIAREQVHRLVLVDETQHLLGVVSLSDILQALVLSPAGIDALGA | |
Gene Information
Entrez Gene ID
Gene Name
protein kinase, AMP-activated, gamma 3 non-catalytic subunit
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0031588 | IEA:Ensembl | C | AMP-activated protein kinase complex |
| GO:0005829 | TAS:Reactome | C | cytosol |
| GO:0005615 | IDA:UniProt | C | extracellular space |
| GO:0004679 | TAS:ProtInc | F | AMP-activated protein kinase activity |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0019901 | IDA:BHF-UCL | F | protein kinase binding |
| GO:0007050 | TAS:Reactome | P | cell cycle arrest |
| GO:0006633 | IEA:UniProtKB-KW | P | fatty acid biosynthetic process |
| GO:0015758 | IEA:Ensembl | P | glucose transport |
| GO:0005978 | IEA:Ensembl | P | glycogen biosynthetic process |
| GO:0008286 | TAS:Reactome | P | insulin receptor signaling pathway |
| GO:0035556 | TAS:ProtInc | P | intracellular signal transduction |
| GO:0061024 | TAS:Reactome | P | membrane organization |
| GO:0006468 | TAS:GOC | P | protein phosphorylation |
KEGG Pathway Links
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR000644 | CBS domain |
UniProt Annotations
Entry Information
Gene Name
protein kinase, AMP-activated, gamma 3 non-catalytic subunit
Protein Entry
AAKG3_HUMAN
UniProt ID
Species
Human
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9UGI9-1; Sequence=Displayed; Name=2; IsoId=Q9UGI9-2; Sequence=VSP_015587; |
| Domain | The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1. |
| Domain | The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP. |
| Function | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK |
| Polymorphism | PRKAG3 genetic variants can be associated with increased glycogen content in skeletal muscle [MIM |
| Ptm | Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. |
| Similarity | Belongs to the 5'-AMP-activated protein kinase gamma subunit family. |
| Similarity | Contains 4 CBS domains. {ECO |
| Subunit | AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non- catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2 (By similarity). |
| Tissue Specificity | Skeletal muscle, with weak expression in heart and pancreas. |
Identical and Related Proteins
Unique RefSeq proteins for LMP000658 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 47132577 | RefSeq | NP_059127 | 489 | 5'-AMP-activated protein kinase subunit gamma-3 |
Identical Sequences to LMP000658 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:47132577 | GenBank | AAH98277.1 | 489 | Protein kinase, AMP-activated, gamma 3 non-catalytic subunit [Homo sapiens] |
| GI:47132577 | GenBank | EAW70656.1 | 489 | protein kinase, AMP-activated, gamma 3 non-catalytic subunit, isoform CRA_a [Homo sapiens] |
| GI:47132577 | GenBank | EAW70657.1 | 489 | protein kinase, AMP-activated, gamma 3 non-catalytic subunit, isoform CRA_a [Homo sapiens] |
| GI:47132577 | GenBank | AHE01766.1 | 489 | Sequence 58303 from patent US 8586006 |
| GI:47132577 | GenBank | AIC51580.1 | 489 | PRKAG3, partial [synthetic construct] |
| GI:47132577 | SwissProt | Q9UGI9.3 | 489 | RecName: Full=5'-AMP-activated protein kinase subunit gamma-3; Short=AMPK gamma3; Short=AMPK subunit gamma-3 [Homo sapiens] |
Related Sequences to LMP000658 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:47132577 | EMBL | CAB65117.1 | 492 | AMP-activated protein kinase gamma 3 subunit [Homo sapiens] |
| GI:47132577 | EMBL | CAE83532.1 | 489 | unnamed protein product [Homo sapiens] |
| GI:47132577 | EMBL | CAE83534.1 | 489 | unnamed protein product [Homo sapiens] |
| GI:47132577 | EMBL | CAE83535.1 | 489 | unnamed protein product [Homo sapiens] |
| GI:47132577 | GenBank | AAH98306.1 | 489 | Protein kinase, AMP-activated, gamma 3 non-catalytic subunit [Homo sapiens] |
| GI:47132577 | GenBank | AAH98255.1 | 489 | AMP-activated protein kinase, non-catalytic gamma-3 subunit [Homo sapiens] |