Gene/Proteome Database (LMPD)

LMPD ID
LMP000672
Gene ID
17304
Species
Mus musculus (Mouse)
Gene Name
milk fat globule-EGF factor 8 protein
Gene Symbol
Mfge8
Synonyms
AA408458; AI325141; MFG-E8; MP47; Mfgm; P47; SED1; lactadherin
Alternate Names
lactadherin; EGF/factor VIII; sperm surface protein SP47; milk fat globule glycoprotein
Chromosome
7
Map Location
7 D3|7 44.9 cM

Proteins

lactadherin isoform 1 precursor
Refseq ID NP_032620
Protein GI 113865979
UniProt ID P21956
mRNA ID NM_008594
Length 463
RefSeq Status VALIDATED
MQVSRVLAALCGMLLCASGLFAASGDFCDSSLCLNGGTCLTGQDNDIYCLCPEGFTGLVCNETERGPCSPNPCYNDAKCLVTLDTQRGDIFTEYICQCPVGYSGIHCETETNYYNLDGEYMFTTAVPNTAVPTPAPTPDLSNNLASRCSTQLGMEGGAIADSQISASSVYMGFMGLQRWGPELARLYRTGIVNAWTASNYDSKPWIQVNLLRKMRVSGVMTQGASRAGRAEYLKTFKVAYSLDGRKFEFIQDESGGDKEFLGNLDNNSLKVNMFNPTLEAQYIKLYPVSCHRGCTLRFELLGCELHGCSEPLGLKNNTIPDSQMSASSSYKTWNLRAFGWYPHLGRLDNQGKINAWTAQSNSAKEWLQVDLGTQRQVTGIITQGARDFGHIQYVASYKVAHSDDGVQWTVYEEQGSSKVFQGNLDNNSHKKNIFEKPFMARYVRVLPVSWHNRITLRLELLGC
sig_peptide: 1..22 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2255 peptide sequence: MQVSRVLAALCGMLLCASGLFA sig_peptide: 1..22 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 2255 peptide sequence: MQVSRVLAALCGMLLCASGLFA mat_peptide: 23..463 product: Lactadherin experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (P21956.3) calculated_mol_wt: 49004 peptide sequence: ASGDFCDSSLCLNGGTCLTGQDNDIYCLCPEGFTGLVCNETERGPCSPNPCYNDAKCLVTLDTQRGDIFTEYICQCPVGYSGIHCETETNYYNLDGEYMFTTAVPNTAVPTPAPTPDLSNNLASRCSTQLGMEGGAIADSQISASSVYMGFMGLQRWGPELARLYRTGIVNAWTASNYDSKPWIQVNLLRKMRVSGVMTQGASRAGRAEYLKTFKVAYSLDGRKFEFIQDESGGDKEFLGNLDNNSLKVNMFNPTLEAQYIKLYPVSCHRGCTLRFELLGCELHGCSEPLGLKNNTIPDSQMSASSSYKTWNLRAFGWYPHLGRLDNQGKINAWTAQSNSAKEWLQVDLGTQRQVTGIITQGARDFGHIQYVASYKVAHSDDGVQWTVYEEQGSSKVFQGNLDNNSHKKNIFEKPFMARYVRVLPVSWHNRITLRLELLGC
lactadherin isoform 2 precursor
Refseq ID NP_001038954
Protein GI 113865977
UniProt ID Q3TDU5
mRNA ID NM_001045489
Length 426
RefSeq Status VALIDATED
MQVSRVLAALCGMLLCASGLFAASGDFCDSSLCLNGGTCLTGQDNDIYCLCPEGFTGLVCNETERGPCSPNPCYNDAKCLVTLDTQRGDIFTEYICQCPVGYSGIHCETGCSTQLGMEGGAIADSQISASSVYMGFMGLQRWGPELARLYRTGIVNAWTASNYDSKPWIQVNLLRKMRVSGVMTQGASRAGRAEYLKTFKVAYSLDGRKFEFIQDESGGDKEFLGNLDNNSLKVNMFNPTLEAQYIKLYPVSCHRGCTLRFELLGCELHGCSEPLGLKNNTIPDSQMSASSSYKTWNLRAFGWYPHLGRLDNQGKINAWTAQSNSAKEWLQVDLGTQRQVTGIITQGARDFGHIQYVASYKVAHSDDGVQWTVYEEQGSSKVFQGNLDNNSHKKNIFEKPFMARYVRVLPVSWHNRITLRLELLGC

Gene Information

Entrez Gene ID
17304
Gene Name
milk fat globule-EGF factor 8 protein
Gene Symbol
Mfge8
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009897 IDA:MGI C external side of plasma membrane
GO:0031012 IEA:Ensembl C extracellular matrix
GO:0005615 IDA:MGI C extracellular space
GO:0070062 IEA:Ensembl C extracellular vesicular exosome
GO:0019897 IDA:MGI C extrinsic component of plasma membrane
GO:0005178 IDA:BHF-UCL F integrin binding
GO:0008429 IDA:MGI F phosphatidylethanolamine binding
GO:0001786 IDA:MGI F phosphatidylserine binding
GO:0001525 IEA:UniProtKB-KW P angiogenesis
GO:0007155 IEA:UniProtKB-KW P cell adhesion
GO:0006911 IDA:MGI P phagocytosis, engulfment
GO:0006910 IDA:MGI P phagocytosis, recognition
GO:2000427 IDA:BHF-UCL P positive regulation of apoptotic cell clearance
GO:0050766 IDA:MGI P positive regulation of phagocytosis
GO:0007338 IEA:UniProtKB-KW P single fertilization

REACTOME Pathway Links

REACTOME Pathway ID Description
5893907 Amyloids
5892763 Disease

Domain Information

InterPro Annotations

Accession Description
IPR000421 Coagulation factor 5/8 C-terminal type domain
IPR013032 EGF-like, conserved site
IPR000742 Epidermal growth factor-like domain
IPR008979 Galactose-binding domain-like
IPR027060 Lactadherin

UniProt Annotations

Entry Information

Gene Name
milk fat globule-EGF factor 8 protein
Protein Entry
Q3TDU5_MOUSE
UniProt ID
Q3TDU5
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; Synonyms=Long; IsoId=P21956-1; Sequence=Displayed; Name=2; Synonyms=Short; IsoId=P21956-2; Sequence=VSP_009880;
Developmental Stage Isoform 1 and isoform 2 are detectable in mammary tissue from non-pregnant animals, with isoform 2 being predominant. Levels of isoform 1 increase during gestation and lactation while levels of isoform 2 decrease. {ECO:0000269|PubMed:2122462, ECO:0000269|PubMed:9920772}.
Domain The F5/8 type C 2 domain mediates high-affinity binding to phosphatidylserine-containing membranes.
Function Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha- v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction (By similarity). Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. {ECO:0000250, ECO:0000269|PubMed:15834428, ECO:0000269|PubMed:18008006}.
Induction Isoform 1 is induced by insulin, prolactin and hydrocortisone in mammary epithelial cells. Expression of isoform 2 is repressed by the same treatment. {ECO:0000269|PubMed:9920772}.
Ptm N-glycosylated. Isoform 1 also exists in both an O- glycosylated and a non-O-glycosylated form. {ECO:0000269|PubMed:9920772}.
Similarity Contains 2 EGF-like domains. {ECO:0000255|PROSITE- ProRule:PRU00076}.
Similarity Contains 2 F5/8 type C domains. {ECO:0000255|PROSITE- ProRule:PRU00081}.
Subcellular Location Membrane {ECO:0000269|PubMed:2122462}; Peripheral membrane protein {ECO:0000269|PubMed:2122462}. Secreted {ECO:0000269|PubMed:2122462}.
Tissue Specificity Mammary epithelial cell surfaces and spermatozoan. Isoform 2 is present in brain, heart, kidney and spleen and at low levels in lung, liver, small intestine and testis. {ECO:0000269|PubMed:2122462, ECO:0000269|PubMed:9920772}.

Identical and Related Proteins

Unique RefSeq proteins for LMP000672 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
113865979 RefSeq NP_032620 463 lactadherin isoform 1 precursor
113865977 RefSeq NP_001038954 426 lactadherin isoform 2 precursor

Identical Sequences to LMP000672 proteins

Reference Database Accession Length Protein Name
GI:113865979 DBBJ BAE30938.1 463 unnamed protein product [Mus musculus]
GI:113865979 SwissProt P21956.3 463 RecName: Full=Lactadherin; AltName: Full=MFGM; AltName: Full=Milk fat globule-EGF factor 8; Short=MFG-E8; AltName: Full=SED1; AltName: Full=Sperm surface protein SP47; Short=MP47; Flags: Precursor [Mus musculus]

Related Sequences to LMP000672 proteins

Reference Database Accession Length Protein Name
GI:113865979 DBBJ BAA35180.1 463 milk fat globule glycoprotein MFG-E8 long form [Mus musculus]
GI:113865977 DBBJ BAE41503.1 426 unnamed protein product [Mus musculus]
GI:113865977 DBBJ BAE28512.1 426 unnamed protein product [Mus musculus]
GI:113865977 DBBJ BAE42127.1 426 unnamed protein product [Mus musculus]
GI:113865979 DBBJ BAE42274.1 463 unnamed protein product [Mus musculus]
GI:113865979 GenBank AAA39534.1 463 milk fat globule membrane protein E8 [Mus musculus domesticus]
GI:113865979 GenBank AAE32714.1 463 Sequence 9 from patent US 5972337
GI:113865977 GenBank AAH03892.1 426 Milk fat globule-EGF factor 8 protein [Mus musculus]
GI:113865977 GenBank AAH03904.1 426 Milk fat globule-EGF factor 8 protein [Mus musculus]
GI:113865979 GenBank EDL07077.1 463 milk fat globule-EGF factor 8 protein, isoform CRA_c [Mus musculus]
GI:113865977 GenBank ACK11749.1 426 Sequence 2 from patent US 7439221
GI:113865979 GenBank AGO74275.1 463 Sequence 1 from patent US 8398984