Gene/Proteome Database (LMPD)

LMPD ID
LMP000680
Gene ID
51422
Species
Homo sapiens (Human)
Gene Name
protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Gene Symbol
PRKAG2
Synonyms
AAKG; AAKG2; CMH6; H91620p; WPWS
Alternate Names
5'-AMP-activated protein kinase subunit gamma-2;,AMPK subunit gamma-2; hypothetical protein
Chromosome
7
Map Location
7q36.1
Summary
AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for PRKAG2

Proteins

5'-AMP-activated protein kinase subunit gamma-2 isoform a
Refseq ID NP_057287
Protein GI 33186925
UniProt ID Q9UGJ0
mRNA ID NM_016203
Length 569
RefSeq Status REVIEWED
MGSAVMDTKKKKDVSSPGGSGGKKNASQKRRSLRVHIPDLSSFAMPLLDGDLEGSGKHSSRKVDSPFGPGSPSKGFFSRGPQPRPSSPMSAPVRPKTSPGSPKTVFPFSYQESPPRSPRRMSFSGIFRSSSKESSPNSNPATSPGGIRFFSRSRKTSGLSSSPSTPTQVTKQHTFPLESYKHEPERLENRIYASSSPPDTGQRFCPSSFQSPTRPPLASPTHYAPSKAAALAAALGPAEAGMLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETE
5'-AMP-activated protein kinase subunit gamma-2 isoform b
Refseq ID NP_077747
Protein GI 100913189
UniProt ID Q9UGJ0
mRNA ID NM_024429
Length 328
RefSeq Status REVIEWED
MLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETE
5'-AMP-activated protein kinase subunit gamma-2 isoform c
Refseq ID NP_001035723
Protein GI 100913192
UniProt ID Q9UGJ0
mRNA ID NM_001040633
Length 525
RefSeq Status REVIEWED
MPLLDGDLEGSGKHSSRKVDSPFGPGSPSKGFFSRGPQPRPSSPMSAPVRPKTSPGSPKTVFPFSYQESPPRSPRRMSFSGIFRSSSKESSPNSNPATSPGGIRFFSRSRKTSGLSSSPSTPTQVTKQHTFPLESYKHEPERLENRIYASSSPPDTGQRFCPSSFQSPTRPPLASPTHYAPSKAAALAAALGPAEAGMLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETE

Gene Information

Entrez Gene ID
51422
Gene Name
protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Gene Symbol
PRKAG2
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0031588 IDA:BHF-UCL C AMP-activated protein kinase complex
GO:0005829 TAS:Reactome C cytosol
GO:0005615 IDA:UniProt C extracellular space
GO:0005654 TAS:Reactome C nucleoplasm
GO:0043531 IDA:BHF-UCL F ADP binding
GO:0016208 IEA:Ensembl F AMP binding
GO:0005524 IDA:BHF-UCL F ATP binding
GO:0004862 IDA:BHF-UCL F cAMP-dependent protein kinase inhibitor activity
GO:0008603 IMP:BHF-UCL F cAMP-dependent protein kinase regulator activity
GO:0008607 IMP:BHF-UCL F phosphorylase kinase regulator activity
GO:0030295 IMP:BHF-UCL F protein kinase activator activity
GO:0019901 IDA:BHF-UCL F protein kinase binding
GO:0006754 TAS:BHF-UCL P ATP biosynthetic process
GO:0006853 TAS:Reactome P carnitine shuttle
GO:0007050 TAS:Reactome P cell cycle arrest
GO:0044255 TAS:Reactome P cellular lipid metabolic process
GO:0006112 TAS:Reactome P energy reserve metabolic process
GO:0006633 IEA:UniProtKB-KW P fatty acid biosynthetic process
GO:0005977 IMP:BHF-UCL P glycogen metabolic process
GO:0008286 TAS:Reactome P insulin receptor signaling pathway
GO:0035556 IMP:BHF-UCL P intracellular signal transduction
GO:0061024 TAS:Reactome P membrane organization
GO:0006469 IDA:BHF-UCL P negative regulation of protein kinase activity
GO:0071901 IDA:GOC P negative regulation of protein serine/threonine kinase activity
GO:0010800 IMP:BHF-UCL P positive regulation of peptidyl-threonine phosphorylation
GO:0045860 IMP:BHF-UCL P positive regulation of protein kinase activity
GO:0042304 TAS:Reactome P regulation of fatty acid biosynthetic process
GO:0019217 IMP:BHF-UCL P regulation of fatty acid metabolic process
GO:0046320 TAS:BHF-UCL P regulation of fatty acid oxidation
GO:0046324 TAS:BHF-UCL P regulation of glucose import
GO:0006110 IMP:BHF-UCL P regulation of glycolytic process
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0016126 TAS:BHF-UCL P sterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
hsa04152 AMPK signaling pathway
hsa04068 FoxO signaling pathway
hsa04932 Non-alcoholic fatty liver disease (NAFLD)
hsa04921 Oxytocin signaling pathway

Domain Information

InterPro Annotations

Accession Description
IPR000644 CBS domain

UniProt Annotations

Entry Information

Gene Name
protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Protein Entry
AAKG2_HUMAN
UniProt ID
Q9UGJ0
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=A; IsoId=Q9UGJ0-1; Sequence=Displayed; Name=B; IsoId=Q9UGJ0-2; Sequence=VSP_000261; Name=C; IsoId=Q9UGJ0-3; Sequence=VSP_015589;
Disease Cardiomyopathy, familial hypertrophic 6 (CMH6) [MIM
Disease Glycogen storage disease of heart lethal congenital (GSDH) [MIM
Disease Wolff-Parkinson-White syndrome (WPWS) [MIM
Domain The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
Domain The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP.
Function AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK
Ptm Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
Sequence Caution Sequence=AAH20540.2; Type=Erroneous initiation; Evidence= ; Sequence=AAS02032.1; Type=Erroneous gene model prediction; Evidence= ; Sequence=BAA84695.1; Type=Frameshift; Positions=228, 233; Note=Frameshifts are upstream of the initiating Met of isoform B.; Evidence= ;
Similarity Belongs to the 5'-AMP-activated protein kinase gamma subunit family.
Similarity Contains 4 CBS domains. {ECO
Subunit AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non- catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.
Tissue Specificity Isoform B is ubiquitously expressed except in liver and thymus. The highest level is detected in heart with abundant expression in placenta and testis.

Identical and Related Proteins

Unique RefSeq proteins for LMP000680 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
33186925 RefSeq NP_057287 569 5'-AMP-activated protein kinase subunit gamma-2 isoform a
100913189 RefSeq NP_077747 328 5'-AMP-activated protein kinase subunit gamma-2 isoform b
100913192 RefSeq NP_001035723 525 5'-AMP-activated protein kinase subunit gamma-2 isoform c

Identical Sequences to LMP000680 proteins

Reference Database Accession Length Protein Name
GI:100913189 DBBJ BAA91962.1 328 unnamed protein product [Homo sapiens]
GI:100913189 EMBL CAE89864.1 328 unnamed protein product [Homo sapiens]
GI:100913189 GenBank AAK00413.1 328 AMP-activated protein kinase gamma subunit [Homo sapiens]
GI:100913189 GenBank AAE62840.1 328 Sequence 7 from patent US 6207148
GI:100913189 GenBank AAP35791.1 328 protein kinase, AMP-activated, gamma 2 non-catalytic subunit [Homo sapiens]
GI:100913192 GenBank AAH68598.1 525 Protein kinase, AMP-activated, gamma 2 non-catalytic subunit [Homo sapiens]
GI:33186925 GenBank EAW53983.1 569 protein kinase, AMP-activated, gamma 2 non-catalytic subunit, isoform CRA_a [Homo sapiens]
GI:100913192 GenBank EAW53984.1 525 protein kinase, AMP-activated, gamma 2 non-catalytic subunit, isoform CRA_b [Homo sapiens]
GI:33186925 GenBank ADC20502.1 569 Sequence 986 from patent US 7638288
GI:100913192 GenBank ADQ31995.1 525 protein kinase, AMP-activated, gamma 2 non-catalytic subunit, partial [synthetic construct]
GI:33186925 GenBank JAA37167.1 569 protein kinase, AMP-activated, gamma 2 non-catalytic subunit [Pan troglodytes]
GI:33186925 GenBank AGD01463.1 569 Sequence 986 from patent US 8338124
GI:33186925 GenBank AHD75948.1 569 Sequence 18331 from patent US 8586006
GI:100913189 GenBank AIC51494.1 328 PRKAG2, partial [synthetic construct]
GI:100913192 GenBank AIC56450.1 525 PRKAG2, partial [synthetic construct]
GI:100913192 GenBank AIC62749.1 525 PRKAG2, partial [synthetic construct]
GI:33186925 RefSeq XP_003318972.1 569 PREDICTED: 5'-AMP-activated protein kinase subunit gamma-2 isoform X3 [Pan troglodytes]
GI:100913192 RefSeq XP_003318973.1 525 PREDICTED: 5'-AMP-activated protein kinase subunit gamma-2 isoform X6 [Pan troglodytes]

Related Sequences to LMP000680 proteins

Reference Database Accession Length Protein Name
GI:100913189 DBBJ BAA84695.1 352 H91620p [Homo sapiens]
GI:100913192 EMBL CAB65116.1 569 AMP-activated protein kinase gamma2 subunit [Homo sapiens]
GI:100913189 EMBL CAB65116.1 569 AMP-activated protein kinase gamma2 subunit [Homo sapiens]
GI:100913192 GenBank EAL24516.1 569 protein kinase, AMP-activated, gamma 2 non-catalytic subunit [Homo sapiens]
GI:100913189 GenBank EAL24516.1 569 protein kinase, AMP-activated, gamma 2 non-catalytic subunit [Homo sapiens]
GI:100913192 GenBank EAW53983.1 569 protein kinase, AMP-activated, gamma 2 non-catalytic subunit, isoform CRA_a [Homo sapiens]
GI:100913189 GenBank EAW53983.1 569 protein kinase, AMP-activated, gamma 2 non-catalytic subunit, isoform CRA_a [Homo sapiens]
GI:100913192 GenBank JAA37167.1 569 protein kinase, AMP-activated, gamma 2 non-catalytic subunit [Pan troglodytes]
GI:100913189 GenBank AGD01463.1 569 Sequence 986 from patent US 8338124
GI:100913192 GenBank AGD01463.1 569 Sequence 986 from patent US 8338124
GI:33186925 RefSeq XP_003270986.1 569 PREDICTED: 5'-AMP-activated protein kinase subunit gamma-2 isoform 1 [Nomascus leucogenys]
GI:100913189 RefSeq XP_003318972.1 569 PREDICTED: 5'-AMP-activated protein kinase subunit gamma-2 isoform X3 [Pan troglodytes]
GI:100913192 RefSeq XP_003318972.1 569 PREDICTED: 5'-AMP-activated protein kinase subunit gamma-2 isoform X3 [Pan troglodytes]
GI:33186925 RefSeq XP_005250062.1 568 PREDICTED: 5'-AMP-activated protein kinase subunit gamma-2 isoform X4 [Homo sapiens]
GI:33186925 RefSeq XP_005551275.1 569 PREDICTED: 5'-AMP-activated protein kinase subunit gamma-2 isoform X1 [Macaca fascicularis]
GI:33186925 RefSeq XP_005551276.1 568 PREDICTED: 5'-AMP-activated protein kinase subunit gamma-2 isoform X2 [Macaca fascicularis]
GI:33186925 RefSeq XP_009202521.1 568 PREDICTED: 5'-AMP-activated protein kinase subunit gamma-2 isoform X1 [Papio anubis]
GI:33186925 RefSeq XP_009452891.1 568 PREDICTED: 5'-AMP-activated protein kinase subunit gamma-2 isoform X4 [Pan troglodytes]