Gene/Proteome Database (LMPD)

LMPD ID
LMP000691
Gene ID
26897
Species
Mus musculus (Mouse)
Gene Name
acyl-CoA thioesterase 1
Gene Symbol
Acot1
Synonyms
ACH2; CTE-1; CTE-I; Cte1; D12Ucla1
Alternate Names
acyl-coenzyme A thioesterase 1; long chain acyl-CoA hydrolase; cytosolic acyl-CoA thioesterase 1; acyl-CoA thioesterase 1, cytosolic; long chain acyl-CoA thioester hydrolase; inducible cytosolic acyl-coenzyme A thioester hydrolase
Chromosome
12
Map Location
12 D1|12 39.0 cM
EC Number
3.1.2.2

Proteins

acyl-coenzyme A thioesterase 1
Refseq ID NP_036136
Protein GI 6753550
UniProt ID O55137
mRNA ID NM_012006
Length 419
RefSeq Status PROVISIONAL
MEATLNLEPSGRSCWDEPLSIAVRGLAPEQPVTLRSVLRDEKGALFRAHARYRADSHGELDLARTPALGGSFSGLEPMGLLWAMEPDRPFWRLVKRDVQTPFVVELEVLDGHEPDGGQRLAHAVHERHFLAPGVRRVPVREGRVRATLFLPPEPGPFPGIIDLFGVGGGLLEYRASLLAGKGFAVMALAYYNYDDLPKNMETMHMEYFEEAVNYLRSHPEVKGPGIGLLGISKGGELGLAMASFLKGITAAVVINGSVAAVGNTISYKDETIPPVTILRNQVKMTKDGLKDVVDALQSPLVDKKSFIPVERSDTTFLFLVGQDDHNWKSEFYADEISKRLQAHGKEKPQIICYPAAGHYIEPPYFPLCSAGMHLLVGANITFGGEPKPHAMAQLDAWQQLQTFFHKQLGSECLHVSPKI

Gene Information

Entrez Gene ID
26897
Gene Name
acyl-CoA thioesterase 1
Gene Symbol
Acot1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 TAS:MGI C cytoplasm
GO:0005739 IDA:MGI C mitochondrion
GO:0047617 IDA:MGI F acyl-CoA hydrolase activity
GO:0052689 IEA:UniProtKB-KW F carboxylic ester hydrolase activity
GO:0016290 IEA:UniProtKB-EC F palmitoyl-CoA hydrolase activity
GO:0006637 TAS:MGI P acyl-CoA metabolic process
GO:0001676 IDA:MGI P long-chain fatty acid metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
mmu01040 Biosynthesis of unsaturated fatty acids
mmu00062 Fatty acid elongation

Domain Information

InterPro Annotations

Accession Description
IPR006862 Acyl-CoA thioester hydrolase/bile acid-CoA amino acid N-acetyltransferase
IPR016662 Acyl-CoA thioesterase, long chain
IPR029058 Alpha/Beta hydrolase fold
IPR014940 BAAT/Acyl-CoA thioester hydrolase C-terminal

UniProt Annotations

Entry Information

Gene Name
acyl-CoA thioesterase 1
Protein Entry
ACOT1_MOUSE
UniProt ID
O55137
Species
Mouse

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=2.6 uM for palmitoyl-CoA {ECO:0000269|PubMed:11694534}; Vmax=1.2 umol/min/mg enzyme with palmitoyl-CoA as substrate {ECO:0000269|PubMed:11694534};
Catalytic Activity Palmitoyl-CoA + H(2)O = CoA + palmitate.
Function Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. True acyl-CoA thioesterase being highly specific for saturated C12-C20 acyl-CoAs, with only a very low activity with decanoyl-CoA as substrate. Most active on myristoyl- and palmitoyl-CoA. Introduction of one or two double bonds decreases the activity to about half. No detectable activity with bile acid CoAs such as choloyl-CoA and chenodeoxycholoyl-CoA. {ECO:0000269|PubMed:10567408}.
Induction In the liver, by peroxisome proliferator (Clofibrate) treatment, via the peroxisome proliferator-activated receptors (PPARs) or fasting for 24 hours. {ECO:0000269|PubMed:11330065}.
Similarity Belongs to the C/M/P thioester hydrolase family. {ECO:0000305}.
Subcellular Location Cytoplasm.
Subunit Monomer.
Tissue Specificity Expressed in heart, kidney, brown adipose tissue, white adipose tissue, adrenal gland and muscle. {ECO:0000269|PubMed:10567408, ECO:0000269|PubMed:11330065}.

Identical and Related Proteins

Unique RefSeq proteins for LMP000691 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6753550 RefSeq NP_036136 419 acyl-coenzyme A thioesterase 1

Identical Sequences to LMP000691 proteins

Reference Database Accession Length Protein Name
GI:6753550 EMBL CAA74327.1 419 acyl-CoA thioesterase [Mus musculus]
GI:6753550 GenBank AAF13870.1 419 cytosolic long chain acyl-CoA thioesterase [Mus musculus]
GI:6753550 GenBank AAH28261.1 419 Acyl-CoA thioesterase 1 [Mus musculus]
GI:6753550 SwissProt O55137.1 419 RecName: Full=Acyl-coenzyme A thioesterase 1; Short=Acyl-CoA thioesterase 1; AltName: Full=CTE-I; AltName: Full=Inducible cytosolic acyl-coenzyme A thioester hydrolase; AltName: Full=Long chain acyl-CoA thioester hydrolase; Short=Long chain acyl-CoA hydrolase [Mus musculus]

Related Sequences to LMP000691 proteins

Reference Database Accession Length Protein Name
GI:6753550 DBBJ BAA32434.1 419 acyl-CoA hydrolase [Rattus norvegicus]
GI:6753550 GenBank AAF13871.1 453 mitochondrial long chain acyl-CoA thioesterase [Mus musculus]
GI:6753550 GenBank AAH03911.1 420 Acot1 protein, partial [Mus musculus]
GI:6753550 GenBank EDL02755.1 453 mCG4931 [Mus musculus]
GI:6753550 GenBank EDL81469.1 419 rCG20735, isoform CRA_a [Rattus norvegicus]
GI:6753550 SwissProt O88267.1 419 RecName: Full=Acyl-coenzyme A thioesterase 1; Short=Acyl-CoA thioesterase 1; AltName: Full=CTE-I; AltName: Full=Inducible cytosolic acyl-coenzyme A thioester hydrolase; AltName: Full=LACH2; Short=ACH2; AltName: Full=Long chain acyl-CoA thioester hydrolase; Short=Long chain acyl-CoA hydrolase [Rattus norvegicus]